how do you know that a micropipette will accurately deliver solutions in DMSO? How stable are pyrophosphates, are they readily accessible, and commercially available?
Accuracy doesn't matter to much here I think. But anyway based on tests I did a while back Eppendorf Pipettes are fairly accurate with DMSO. Not so with lower boiling solvents.
This procedure would be a lot faster if he just had 5 P1000's pre-dialed to the volume that he requires - there's no sense readjusting the volume over and over especially if this procedure is used on several substrates!
28:00 no keck clip on the rotovap
More of these please!
26:51 just contaminating the whole bottle of sodium sulfate
how do you know that a micropipette will accurately deliver solutions in DMSO? How stable are pyrophosphates, are they readily accessible, and commercially available?
Accuracy doesn't matter to much here I think. But anyway based on tests I did a while back Eppendorf Pipettes are fairly accurate with DMSO. Not so with lower boiling solvents.
why don't you just filtered the reaction through a plug of celite in a glass funnel with a cotton plug? filter paper leaks much more easily.
#unclampedfilterflask
This procedure would be a lot faster if he just had 5 P1000's pre-dialed to the volume that he requires - there's no sense readjusting the volume over and over especially if this procedure is used on several substrates!