Agarose gel electrophoresis

In this video we have described the practicals approach of the agarose gel electrophoresis. The agarose gel electrophoresis can be used to check the quality and quantity of the macromolecules such as DNA , RNA and proteins. Here in agarose gel electrophoresis the separation depends on charge and molecular weight and in some cases also depends on the conformation of the macromolecules. The agarose gel electrophoresis is routinely used for the analysis of PCR products, molecular markers, genomic DNA, Total RNA and proteins. In short the agarose gel electrophoresis is heart of the biotech and life science students who is working on the DNA, RNA and proteins.
Here we have explained the most of things and minute details but due to time limit and the topic is vast so we have tried our best to cover the topic …!!!!!!!! THANK YOU !
Credits
Video shot by - VInayak Mohandas 👌
Assisted by - Anusha Raj
Edited by -ME 🙈

[@aparnars8877]

Well explained.. Kudos to the team..😊👏

[@aaryavyawahare1737]

Very well explained 🙌

[@EarthC1_37]

Nicely explained🙌🏻🙌🏻thank you sir

[@saviofranklin7903]

Well explained .. 💯❤️

[@aparnarr4304]

Thank you for useful information 🙏

[@pateljyothi2238]

Chala baga explain chesav anna tq

[@wajeehali828]

very good brother , love from Pakistani ,Now Asia will rule ,West will follow .

[@lifereplicon]

Pls do share, it will encourage me to post more ! Thank you brother 😃

[@abdulmueez4081]

💀?

[@nafeesavhora6226]

Thank u..This video helps me a lot...✨🙂

[@CyberRager]

Very informative video, keep it up, greetings from Germany!

[@sruthisuresh2527]

👍🏻👍🏻

[@nadeemiqbal4742]

Thank you sir.

[@user-rj1in9wq4o]

very good explanation

[@reshna4387]

🔥🔥

[@sravanireddy2139]

👏👏

[@aryasnair6365]

👍👍👍

[@ajinpasok]

👍👍

[@cursedzodiac272]

Now i understand ..thank you

[@lifereplicon]

Pls do share it will encourage me to post more ! Thank you 😃

[@Itz_me_nishith]

Thank you so much.❤ Tomorrow is my biotechnology board practical 🙏🏻

[@lifereplicon]

All the best 🙌

[@thegist-happylearning4124]

👌✌️👏👏❣️

[@mylifeasloveline7368]

Thank you

[@karunakrishna5953]

Very much useful 👍👍 Pls upload more videos like this 🙌

[@lifereplicon]

Sure 😀

[@Biotec190]

Well explain bt background sound creats difficulties

[@Lalalalalalalala231]

THank you ❤✌
You cleared all my doubts ⚡

[@lifereplicon]

Pls do share it will encourage me to post more ! Thank you 😃

[@chandramohands]

Dear, its well explained....good to use "Gel Red" rather than 'Ethidium bromide'. gel red is very safe.

[@lifereplicon]

Thank you sir for your response in my previous lab i was using ETBR, currently we do use gel red only its very safe i agree and i do recommend !

[@aartiajani2952]

🙌🙌

[@user-po9rl7zo3r]

Thank you for your explanation 🙏🏻✨

[@lifereplicon]

My pleasure 🥰

[@sangitasonagara691]

superb...
thank you 🙂

[@lifereplicon]

Keep watching

[@snehachakraborty9801]

Very nice explanation!

[@lifereplicon]

Thank you 😊 pls do share with ur friends family

[@monishnb3923]

thank you sir so much... tomorrow i have board biotech practicals and you completely cleared all my doubts on how to do the experiment

[@lifereplicon]

All the best 😉

[@creativegenius3254]

Thank you brother🙏kudos to you

[@lifereplicon]

Thank you 😊

[@nareshbarik5384]

Thank you ❤🙏

[@lifereplicon]

Welcome!

[@asmashaheen5372]

Well explained brother keep it up from Pakistan

[@lifereplicon]

Thank you dear 😊

[@rangitha.r52]

Tq sir 🙏🏼🙏🏾

[@snehakrishnankutty2666]

Its a good explanation sir... Keep up it up❤🎉

[@lifereplicon]

Thanks a ton

[@srivalli41]

Wow nice 😯😲

[@lifereplicon]

Thank you

[@inshiasticvines4037]

It was really nice.

[@sushmayadav3329]

It's very well explained practical..

[@lifereplicon]

thank you pls do share with your friends ☺️

[@sushmayadav3329]

@@lifereplicon ya definitely.. I already done

[@lifereplicon]

Thank you 😊 really appreciated 😃

[@user-xi6vr9xb5n]

well explained😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍😍. thank you

[@lifereplicon]

Thank you 🙏

[@vinayakmohandas1081]

✌✌

[@pagalpintu9846]

Very useful.... & i request u to kindly use a special microphone for yr clear voice.
Thankyou 🙏🏼

[@lifereplicon]

Thank you 😊 for suggestion i. Will implement it

[@r.k7142]

Sir I need more videos which is imp in biotechnology field (techniques)

[@lifereplicon]

Sure 😃

[@nehanagrale9994]

Sir I want to learn about crossed over gel electrophoresis

[@justmusic6591]

Very useful please upload SDS page also

[@lifereplicon]

I will upload

[@SaumyaSharma123]

Where is the gel image sir?

[@lifereplicon]

[e-mail] pls dm so i can share

[@arkamitramajumder3660]

Will it mix properly by centrifuger? And can we use vortex for this? Will it break those dna or not?

[@lifereplicon]

It will mix with brief centrifugation no need to vortex

[@mubinabanu9054]

Hi I'm having a doubt. How much 6x loading dye and DNA was added to the gel

[@lifereplicon]

Don't add to the gel 😐

[@dhairyatrivedi2306]

Very nice explanation. BTW who are from 12th sciences

[@lifereplicon]

Sorry I didn’t get you ?

[@dhairyatrivedi2306]

Brother i am student of 12 th science and in biology we have DNA fingerprinting in which there is topic of gel electrophoresis

[@lifereplicon]

@dhairyatrivedi2306 i hope u understood !

[@dhairyatrivedi2306]

Yes thanx very nicely explained

[@venkateshjeedimetla1471]

Every thing is very perfect but we can't hear your voice perfectly and moreover captions are not available so please make sure that your voice is clear to understand your important information sir

[@lifereplicon]

Thank you i was on low budget during that time

[@venkateshjeedimetla1471]

@@lifereplicon may God solved your budget problem now and wishing you to rock amazingly in TH-cam

[@lifereplicon]

Pls do like s SHARE and subscribe ! I need support

[@microcolony6835]

Why two types of buffer used here ? If i use any one kind of buffer ( TBE/TAE) is any problem

[@lifereplicon]

Not at all you can any buffer which is available for you
Thank you

[@microcolony6835]

@@lifereplicon ok sir

[@microcolony6835]

Is TAE or TBE buffer is called running buffer?

[@lifereplicon]

both are running buffers

[@swastika2147]

How much ethidium bromide did u add to the gel?

[@lifereplicon]

0.5ug/ml final conc

[@VIKASHKUMAR-qq9de]

Bhai tum ye batao dye jb tum insert kr rhe to vo dikha na

[@lifereplicon]

Sab dhikaya hai na sir

[@muhammadmuneer6644]

Good but voice is not clear

[@lifereplicon]

I will improve thank you for suggestions

[@areejnawaz7971]

JUST IMPROVE VOICE QUALITY REST U R BEST

[@lifereplicon]

Thank you

[@riya8910]

etbr is added to buffer?

[@lifereplicon]

No its added to melted gel

[@riya8910]

@@liferepliconin well?

[@lifereplicon]

No in gel

[@riya8910]

It can be added , after whole process is done ...?
For just visualisation

[@lifereplicon]

No it should be mixed throughout the gel as the DNA or RNA. Can be visualise at anywhere in the gel