Part-1How to avoid contamination in Tissue culture/How to operate autoclave for proper sterilization

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  • เผยแพร่เมื่อ 11 ม.ค. 2025

ความคิดเห็น • 14

  • @sugandhapant6937
    @sugandhapant6937 3 ปีที่แล้ว +1

    You're doing a great job sir 🙏

  • @srinivasmohanty8859
    @srinivasmohanty8859 3 ปีที่แล้ว

    Amazing explained bro

  • @mudavathpadma4976
    @mudavathpadma4976 3 ปีที่แล้ว

    Thku so much sir 😊

  • @greenhut1642
    @greenhut1642 ปีที่แล้ว +1

    IS IT POSSIBLE TO AUTOCLAVE IN NORMAL PRESSURE COOKER,

    • @Dr.Rakesh.B
      @Dr.Rakesh.B  ปีที่แล้ว

      Yes its possible.
      Keep it in high flame for 3-4 whistles and leave it in simmer for 15 minutes and turn off.
      We have done many times in cooker, tried and tested method.

    • @greenhut1642
      @greenhut1642 ปีที่แล้ว +1

      Thankyou sir for the reply,

    • @greenhut1642
      @greenhut1642 ปีที่แล้ว

      How can I contact you in WhatsApp, is it possible to assist me in tissue culture. Presently I had collected all materials for tissue culture. For doing it in my home.

    • @greenhut1642
      @greenhut1642 ปีที่แล้ว +1

      i done with pressure cooker but the solution didnt solidify, 4gm agar i had used in 500ml,some precipitate were also seen after boiling. is bacteriological grade agar is used

    • @Dr.Rakesh.B
      @Dr.Rakesh.B  ปีที่แล้ว

      @greenhut1642 Its not because of pressure cooker. Add 5 to 6 gm of agar. Change agar and check once.
      3 whistle and 15 minutes simmer.
      It works great