Is every seed produces phenolic compounds? I have prepared the germination of seeds of mustard,gram etc but didn't seen the brown colour in media . BTW in the lab u r alone working!! What r u doing bro?
A question sir, with the addition of the activated carbon, what happens to the PGRs needed in media? Do the Auxins, Gibberellins & Cytokinins not get absorbed? Also can you please provide the papers for us Novices, thank you sir
Yes, a little amount of hormones do get absorbed. Thats why we keep changing media every week. And we stop adding charcoal after few cycles. You can contact me at rakeshb96@yahoo.com for reference papers
Just autoclave for decontamination and wash with slight detergent and rinse with distilled water and dry it in hot air oven at 50 - 60 degrees for a day. Watch my video on how to avoid contamination for more ideas
Keep uploading more videos like this , sir👍
Thanks Bro for sharing the process and techniques of in-vitro seed germination . I want to give 100 likes....
Nice video
👌👌👌
Possible to explain how to use adenine suphate to avoid phenol of explant
Awesome stuff!! Keep it up bro
Sir for coated seeds which type of nutritional test are done???? Like protein.... ???
Sir. Please make one video for how seed is coated with microbial strain which protocol is used??
Nice video bro..
Sir. I want agrobacterium c carrot 🥕 hairy root.. video.
Thank you so much..
what is that "plant tissue culture" is there any way to make that at home?
Yes so many people do that at home for passion as hobby. In Facebook there's many group you can search and join to that
Is every seed produces phenolic compounds? I have prepared the germination of seeds of mustard,gram etc but didn't seen the brown colour in media .
BTW in the lab u r alone working!! What r u doing bro?
A question sir, with the addition of the activated carbon, what happens to the PGRs needed in media?
Do the Auxins, Gibberellins & Cytokinins not get absorbed?
Also can you please provide the papers for us Novices, thank you sir
Yes, a little amount of hormones do get absorbed. Thats why we keep changing media every week. And we stop adding charcoal after few cycles.
You can contact me at rakeshb96@yahoo.com for reference papers
Can tell me washing bottle techniques
Just autoclave for decontamination and wash with slight detergent and rinse with distilled water and dry it in hot air oven at 50 - 60 degrees for a day. Watch my video on how to avoid contamination for more ideas
Which hormone used
Giberellic acid
Sir can u please sujjest me the best composition of Ms media for tomato seed culture invitro ..
@@Dr.Rakesh.B thank you 😊
I want to read UR reasearch paper sir.....
You can follow me in research Gate (Rakesh Bhaskar) or Google Scholar (Dr. Rakesh)
👍👍👍👍👍👍👍
Why we autoclave Hgcl2?
The water we use to prepare the solution might have some contaminants. So its advised to autoclave