Precisionary Webinar: Claudia Loebel on "Precision Cut Lung Slices (PCLS) for Pulmonary Fibrosis"

Claudia Loebel, M.D./Ph.D. is an Assistant Professor of Materials Science & Engineering and a Biological Sciences Scholar at the University of Michigan, US. She obtained her MD (2011) at the Martin-Luther University Halle-Wittenberg in Germany and PhD (2016) at ETH Zurich (Switzerland), before completing a postdoctoral fellowship in the laboratory of Professor Jason Burdick at the University of Pennsylvania.
She was awarded the Pathway to Independence Award (K99/R00) through the National Heart, Lung, and Blood Institute at NIH for her work on synthetic lung tissue models to probe mechanisms of alveolar epithelial cell dysfunction. Her research involves the development of PCLS to probe mechanisms of early epithelial cell differentiation during lung injury and fibrosis.
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In this webinar, Dr. Loebel will:
✔️ Describe the technical development of PCLS with a focus on epithelial cells
✔️ Explain the necessary culture conditions for optimal PCLS in pulmonary fibrosis research
✔️ Discuss the imaging and quantification of PCLS metrics in pulmonary fibrosis
Original webinar date: January 17, 2023
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0:00 Introduction
1:54 My group works at the interface of materials engineering and mechanobiology
4:04 What is the regenerative capacity of the adult lung?
7:13 The distal lung is a facultative regenerating tissue.
10:27 The basic components: a defined matrix and the cells
15:35 Micro-structured hydrogels preserve surfactant protein expression and maturati
18:13 Orthotopic transplantation of dissociated human alveolospheres into mice
22:03 Pulmonary fibrosis leads to increased stiffness in the alveolar space
25:48 Alveolospheres form polarized structures in 3D
27:40 Pulmonary fibrosis (PF) is a progressive respiratory disease currently with
28:00 Pulmonary Fibrosis is an irreversible inflammatory response to injury
31:30 Developing a PCLS model to investigate epithelial crosstalk and differentia
33:14 PCLS enables the culture of Alveolar Type 2 and Alveolar Type 1
33:54 AT2 cells spread during PCLS culture
37:15 Troubleshooting: same-day isolation and slicing yield higher viability.
38:36 Troubleshooting: Media Conditions - Troubleshooting (without FBS)
41:25 PCLS is a powerful tool to investigate epithelial differentiation in-vitro
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#lungs #lungslices #pulmonaryfibrosis #pulmonary #fibrosis #claudialoebel #biology #NIH #PCLS #epithelialcells #epithelial #compresstome

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For More info on the Precisionary Precision Cut Lung Slices protocols, please visit precisionary.com/support/resources-menu/experimental-protocols/.
For info on the Compresstome vibrating microtome, please visit precisionary.com/main-products-page/compresstome-product-page-menu/vf-510-0z/.