Bradford assay principle explanation

Bradford assay principle explanation - This lecture explains about the bradford assay to determine the total protein concentration in a cell. This is a technique for the total protein test.
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I've tried so hard to understand Bradford assay, and I was looking for an easier way to understand it, and I finally found it. You're the best! I really like your videos.

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[@ashwaqalmutairi3779]

More than perfect. I've tried to find a simple explanation for this assay, and this is it.

[@sapphiregem12]

Thank you so much for this video! I have a lab report about Coomassie blue dye and this video really helped clarify how the protein-dye complex comes about in a way that is easy to understand.

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thank you for simplifying the explanation. thank thank thank. very comprehensive.

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Occam's razor mhmm

[@jianingliang6439]

Really good explanation. Even English is not my fist language, I got to understand the principles. Helps a lot!! Thanks

[@vitormotta781]

Thanks for the explanation, it was very helpful. Great job!

[@purandarsarkar7764]

very much useful for me. i hope sir, u will talk about that SDS problem in more depth in future... Thanks

[@juice28rsx]

great job explaining!!! i swear when my professor was explaining this i thought he was speaking german but now i def get it thanks again

[@ftYamell]

thanks to you sir,for explaining this so clearly !

[@sushmakapoor5742]

sir...plz also explain the lowrys method of protein estimation...
nd one more thing...u r the best..👍👍..ur language is very easy nd u r the last minute saver in our exams..

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great help before practical exams!! thanks a lot for the explanation Sir

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Thanks for the explanation!!

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[@dhritinayyar7123]

Well explained. Keep it up!

[@hassaneinshaban6077]

Thanks for the well organized lecture, what are advantages of bradford method for detecting proteins?

[@user-hk6qu7rq2n]

thank you now I Know the exact principle behind a Bradford assay thank you!

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Thank you for the videos you make. May you explain the calculations concept of BCA assay also, by using excel.
Please 🙏🏻

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EASY + HELPFUL, really thank you

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Thank you so much for this great explanation ......😊😊😊 You are a fabulous teacher.....

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Really good explanation ,thanks
D:

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Great video, helped me present at Lab meeting. Thanks!!!

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[@souvikdas3319]

wonderful explain thank you sir

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Thank you.

[@Mohitthakurofficial]

Please also explain how to generate a standard curve practically for both Bradford and Lowry assays, and my second question is how to know the concentration of samples once we have the absorbance of each (is it by using Lambert-Beer Law?).
My third question is about Standard Dilutions (What is it, do we make it and for what purpose?).
My last question is please explain these terms to me (Standard solution, control, treated, etc).

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Thanks, it is awesome. :D

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Thank you , Sir. It really helps me. Thank you so much

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[@serajulislam7448]

So nice!!

[@ae3262]

Thx a lot😭, you help so much for my persentation of Bradford!❤

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[@AZ-qx1xd]

Thank you Shomu, it was helpful!

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[@sharmilasenthil928]

Hi sir, thank you for your wonderful lecture, and I have one boubt... In this assay we measured absorbance at 595 nm, but in uv visible spectrum, blue or voilet will absorb at only 380- 430, then what is the mechanism behind this assay???

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Thank you so much! It really helps my study!

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[@panzigaming4564]

Please make a video on protein estimation on Lowry folin method as soon as possible

[@dr.meathany6677]

thanks 🙏

[@sandaasstudios]

Hi! Could you please explain why CBB in its unstable state is considered a cation when it actually has a lone pair of electrons, but is considered an anion once it donates its electron pair?

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awesome !! thank you so much !!

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[@prabhakar1494]

Will Triton X-100 hinder with the binding of dye and protein. Triton x-100 is non ionic detergent right?

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Really good. thanks

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thanks a lot

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thanks shomu

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[@nikksadams3267]

super explained

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thank you .....very nice ...video

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[@sushreeacharya5510]

Thank you sir

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[@vijaynimkandeneeri6117]

Nice is Bradford reagent light sensitive..? Im doing in dark but still I m getting higher absorbance in blank? Can you plz help to resolve this problem?

[@fatimafalak37m89]

Sir please explain standard curve of Bradford protein assay

[@Hiiraatif]

Thank you.

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[@mohiniverma9710]

can u tell me that change in color can also be generated if protein is degraded?

[@random.shorts.0.]

Well explained 👍🏻

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Thank you

[@dominiclunn123]

If the red unstable form is predominantly negatively charged in this state, then why is it 'cationic' seeing as I thought an ion with a net negative charge was an anion?

[@JoseCruz-en2ev]

The CBB has many groups on its structure. In an acidic medium, all the three nitrogen atoms in the CBB are protonated and so they carry a positive charge, and the two sulfonic acid groups are negatively charged, so the whole molecule will carry a net positive charge. However, the molecule in this stage can still give a lone electron pair to the protein. When the molecule gives a lone electron pair to the protein both of them become unstable and they bind to each other by ionic forces coming from the electron pair being given to the protein making the CBB electronegative and the protein electropositive in the dipole moment. The interaction then is strengthen by the VDW forces. It is a very specific and complicated chemical reaction. I hope I answered your question.
response from Biomedical and Biological Sciences

[@EagleKiGiggleStories]

Jose Cruz its very nice answer

[@abdulazizabdunasimov8978]

Thanks for clarification

[@javeriaaltaf]

@@JoseCruz-en2ev let me clarify please, if CBB is already in cationic form ,then how could it be electronegative later? and amino group already have lone pair they must be protonated to get positively charge and here protein in taking lone pair of electron, ambiguous

[@americanflower6859]

Amazing

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[@rutikghagare4992]

Sir but when Brilliant blue Has red colour it is in cationic form why there is negative charge then 🤔?
I video is very helpful

[@fawadali2416]

I have a simple question if the dye is in acidic condition how will it donate electron as in acidic condition it will act as an acid and acid are proton done not electron... Plz somebody can explain

[@topperslibrary7662]

explain lowery phenol method ......its very confusing so kindly make a vedio on tjis topic

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thank u👌

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better than professor like wow.

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really helpful and easy to follow :)

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[@chand9576]

I have a doubt, you told that in cationic form CBB is unstable and we know that in cation have +ve charge then why did you show CBB with -vely charge when it is unstable.

[@divyachauhan8257]

Why we are using only bsa protein .why we are not using another protein for this assay as standard protein?

[@mahnoorshahzad5873]

why we set spectrometer at 595 nm

[@sung-wookher4381]

LEGEND

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Thank you

[@rukmini0712]

I need anionic dyebinding method .......

[@aakashkulkarni4979]

plz shomu ..make video on microbiological assay ...1 diffusion assay 2 turbidometric assay 3 metabolic response assay enzymatic assay

[@shomusbiologyofficial]

+COC Lover thank you for topic suggestions. I will make that soon.

[@nikzcool07]

how can we remove sds from protein sample

[@malakbecher2583]

we Can use Bradford assay to detect the NOx concentration

[@siddheshgawde8013]

Sir can you please make video on Folins lowry method

[@shomusbiologyofficial]

Okay

[@siddheshgawde8013]

@@shomusbiologyofficial Thank You

[@divyagupta2131]

Please make video on lowry method also

[@shomusbiologyofficial]

Okay

[@divyagupta2131]

@@shomusbiologyofficial tq sir😊

[@giavinh95]

can you add eng sub?

[@sivajo2908]

Why specifically using BSA as standard

[@calvincerashidkawawaa.6062]

Clearer and simpler

[@shomusbiologyofficial]

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[@maanvikhairkar1191]

Very well explained. But this video should have been named comassine brilliant blue and not Bradford

[@shomusbiologyofficial]

Okay. Thank you

[@bhaskarsharma9002]

Bhai Bradford assay ka Graf be bato

[@gasperkosmac7672]

What did you study to know so much about this stuff. Did you learn it yourself?

[@shomusbiologyofficial]

+Gasper Kosmac I learned it myself studying

[@intisarmohamed2638]

i had an assement ineed help

[@SattarJayed]

I beg your attention-
According to Lewis' Acid-Base theory-
a) An acid is an electron pair acceptor.
b)A base is an electron pair donor.
Now, you said that CB blue's unstable state is ACIDIC. but in its mechanism you said that , CB blue donates lone pair of electrons! Doesn't this contradicts Lews' Acid-Base theory?

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+mineral water great. Glad you liked my lectures

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Speaks alot extra 😡 you just needed to explain the topic. Not about it's four ancestors

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Thanks alot

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