Stuff like this is what motivates people to become biotechnologists. Making whatever you want, playing with organisms and modern technology creating something that sounds like magic
And the amazing thing is that all of this can actually be what your job is like, depending on the specifics. For example, a bio laser could be used in experimental cancer treatment, advanced brain scanning technology, indicators that literally point to the location, and more.
Im a computing scientist and I'm disappointed we don't use more lasers. Where are my optoelectronics ? I mean, I know about photolithography which is basically how every chip is made ever. Still , want to play with lasers .
the fact your laser made of "trash" worked better means the fates have decided you are a garbage bender now. you made too many amazing things from scrap that it's your medium now. o7 keep up the amazing work
Thanks for this. Almost 40 years ago in 8th grade I had the assignment to 'design' a biological creature. I had been obsessed with dye lasers from a library book on how to make your own (I later dug into research and Amateur Scientist articles), and imagined a creature with a biolaser built in, with a flash lamp pumped with the biology of an electric eel, with fluorescent liquid similar to a jellyfish, biolenses of a squid's eye, and partially reflective material similar to a cat's iris or the skin of some fish. The teacher was very critical and said it was so improbable. But that wasn't the assignment, and I remained convinced all this time that all the components were possible, with occasional flashes of vindication coming out, including this one. I still have the desire to build a dye laser so I will have to pop it a little higher in my project list.
Things will get interesting once we get to the stage where we can do fully simulated generative DNA design and program in biological components as needed. I think we have the tools to start working on this now.
I have worked with dye lasers and want to share some insights: - You do not need UV light to pump Rhodamine 6G. The frequency-doubled output of a Nd:YAG laser (532 nm) is typically used and is much more efficient since the quantum defect to the emission wavelengths is small. - A pumped stagnant dye solution will quickly get trapped in states that are unresponsive to pump light. The dye solution is therefore typically circulated so that the dye molecules have time to return to the ground state before being pumped again. - The optimal dye concentration depends on the length of the cuvette with longer cuvettes needing less concentration. The concentration also shifts the tuning range of the dye with higher concentrations shifting to higher wavelengths. - The surfaces of the cuvette can themselves act like a laser resonator, resulting in an unwanted beam. The cuvette is typically vertically tilted to steer that beam away from the intentional one. - The positioning of the cylindrical focal lens is quite important as you have correctly identified. However, tilting the lens like that results in aberrations that lead to uneven illumination. A better solution would be to move the whole lens closer or farther away from the cuvette. Where the lens needs to be rotated is around the axis set by the pump beam. This tilt angles the focal line. You can either align the focal line with the laser resonator for maximum conversion efficiency or tilt it slightly away from it to limit the amount of amplified spontaneous emission that travels along with the dye laser beam. - Rhodamine 6G is indeed a nice dye.
Do you need to lock the 532 or just a simple output of a laser pointer (the ones sold on e.g. AliExpress is enough)? What are the powers for it you think? The techniques he used in the video are for pulsed lasers afaik, would be more interested in CW
The 'technicalities' of the desire to transfer knowledge, while at the same time avoiding a channel/video takedown because of 'Karens' (or atmospherically geometrically frozen water).
plus his mum said ,' don't do that ' many times ,so he is naturally programmed to have to try it , knows we are too ,human nature ..ever since Eve actually ,When the Big Boss ,said don't eat that trees fruit it will kill you , what did she jolly well go and do !!??.
I came to a dead stop at “huge collection of genetically engineered E. coli.” To a guy with thousands of books, parts for obsolete computer architectures, and a ton of other random stuff “too good to throw away,” that seems weird. Yet you did something with it. I’m envious.
Literally synthesized molecules in an abandoned farm house for 2 years trying to make one of these dye lasers. And the meat laser? Way farther than i ever could achieve. I made 3 dyes but i could never get them into lasers. I didnt have ambition, or 3d printing but man. This was literally my dream as a 17 year old. Thank you for reminding me of the days of gold when chemistry channels like nurdrage and chemplayer and masterminds like styro the like Began to inspire me. You really rock, bro.
@@dontworry4945 So, a whole celebrated book is devoted to the concept of creating life, in which case a monstrosity is born, but when we do it today and answer a thousands-year old question of where do we come from, we're just, like, "meh"?
@@Noconstitutionfordemocrats1 granted, I curse in 6 languages fluently and was raised speaking both UK and US English, somehow I didn't get creating life out of synthesizing molecules. For fuck's sake, burning hydrogen synthesizes water molecules! That sure as shit isn't life, but absolutely necessary to maintain it. Of course, synthesizing organic chemicals properly, that's a wee bit more complicated.
As one of my optics profs once said: almost everything will lase if you pump it hard enough. Also, and without getting into the intricacies of building a high end laser, a simple trick to make the laser cavity more stable (which should make it easier to align) is to replace one of the end mirrors with a slightly convex mirror (i.e. with focal length larger than your cavity length).
Yeah, creating a more stable (as in stability polynomial, not necessarily acoustically) cavity is to provide at least *some* form of focusing. Also for anyone else interested in doing this themselves: Just because the commercial UV laser was *safer* (electrically) to operate it's STILL A UV LASER which means it basically automatically is a class 3 or higher laser and also carries a higher risk of cancer (I am not entirely sure if this is wavelength dependent, but in all laser safety briefings and classes our magical wavelength is 400nm and everything below that requires *very* hard to get safety gloves and thorough documentation for something like 40 years due to the potential increase in cancer risk if exposed to the beam)
@@reinei1 you're right it's wavelength dependent. UV light is just the right energy level to cause particular mutations in our DNA (two bases covalently bond together), whereas visible light is too long wavelength. I'm guessing high power visible light would just burn a hole in your skin before causing cancer
@@reinei1 Electrical Interlocking and MAJOR Safety Precautions are for nerds though… ( /j , the people behind this channel are probably doing all that / fine, but yeah especially the diode lasers selling these days really pretend laser safety doesn’t exist)
@@kadrix732 Yes, I am. I'm smart enough not to associate something like this with a sex toy. You'd have to have a pretty low IQ to make that association.
Dude, if I had known that you needed dyes I would have sent you some of every kind that I have on hand for free. I really need to get up with you on donating some stuff one day. As a chemical distributor, I have tons of dyes, just like these, that you could just have. Take fluorescein for example, I have pounds upon pounds of the pure powder, plus tons of others. If you ever need anything like that, just let me know and I'll gladly send you a ton of each absolutely free. 😃
Thank you all for the encouraging remarks! I will reach out when I find the time to do so and I will try to donate some chemicals and elements to his channel/business to help save him some money and to just help out. Ignore the guy wanting to make drugs. I get comments like this occasionally due to some of the things that I sell and I do not appreciate those types of comments and I report any suspicious people to the feds because I am required to do so by law in order to be able to keep selling such things. Don't want to get a visit? Don't ask me to help you make drugs. Simple.
I built one of these when I was 13 not realizing how dangerous they were, I am still surprised and thankful I did not get electrocuted or blinded doing it!
I can only imagine what your ultimate goal could be with all of this, I suspect that a lot of this stuff has already been played with in the big labs, but the fact that you are doing it here, just proves that ingenuity and the masses can prevail. Best to you, Ken.
This video really takes me back about 35 years, when a friend and I puttered around with both N2 lasers and fluorescent dyes in our off time. Something that's not mentioned but should be kept in mind - Rhodamine 6G will stain *everything* you get it on, so aprons or other cover should also be worn in addition to PPE for the risks that will affect your health and not just your clothes.
"I've done so much with so little for so long I can now do anything with nothing" I saw/read this many decades ago and thought I'd share in case you haven't read/heard. Very appropriate for you imo. Something I tell my kids and others, "There is nothing hard, just things you haven't learned yet" Enjoyed very much, be safe and hf learning.
Downright kaleidoscopic rush of a video, and all of it was actual genuine examples of what amazing, beguiling thing you're working on! _No wonder_ I'm subscribed to you guys.
I graduated in physics, but I have a general understanding of most fields of science (nerd with adhd)... Anyway, genetic stuff is literally magic to me. I'm still not 100% convinced that bio engineers aren't just wizards.
I'm a laser physicist by trade and this is a fantastic video. Most of it is familiar, but i'm very impressed by how well you synthesize all these different topics and condense them without necessarily reducing them to an inaccurate form. Specifically, the topic of grating based mode-matching is awesome and a bit of a pain to explain well, but you managed to do it in less than 30s! One critique is that the TEA doesnt work via stimulated emission, but rather ASE (amplified spontaneous emission). This also means that although it is fairly temporally coherent, it isn't perfectly so which is why it only runs on a pulsed source.
"I maintain a huge collection of genetically engineered E Coli" belongs in an xkcd somewhere Thanks to StyroPyro I immediately recognized the TEA laser and am immediately scared. Also that feeling of building it all proper like and it works worse than the prototype is so much feels.
This gives me the same vibes as writing my engineering honours project, you've clearly done tonnes of amazing work and have absolutely no time to show us the immense amount of work you had to do to get to where you are. Very interesting and exciting video, loved it!!
i like that you used this one paragraph compliment to… brag about yourself. like your first sentence added exactly nada to the comment, and makes you look socially iffy
@@thethoughtemporium Please do some experiments with a Kozyrev Mirror. I would really like to know the truth in what you experience. Apparently according to the team who work with it and others it seems to really concentrate time and the experience is horrifying to some.
I discovered Les's Lab few days ago, and what he does is very impressive, but also shows how some of these things are not as complex as they might initially look. I knew of your channel for years, but this is a great showcase of lasers. The dye lasers are cool. It is also mind blowing that anybody can just engineer DNA of a bacteria with just few clicks in a browser. Wow.
Damn that's amazing, those colors are so beautiful. I miss my days in the spectrometry lab. Looking forward to when you can sear your initials into meat with... umm... meat. :)
It's weird seeing a video on such a sketchy laser machine and not be reminded how it'll delete my retina if i even think about looking at the the spot on the wall
Bro I didn’t even know you could do almost any of what you talked about, that’s super cool, like ordering custom dna things that grow with your desired codes or whatever you called it, wild man, keep it up
"We really don't recommend you try to build one, seriously it's super dangerous and could easily kill you in an instant. So anyway, here's exactly how you could build one."
This is making me think about that silver nanoprism video from applied science a while back, and that’s one I’ve gone back to quite a few times. Great video, love this stuff as always
Something I don't see anywhere which should be shouted from the rooftops. WHERE EYE PROTECTION WITH LASERS. Not sure if this is the type that needs it if it's a special case, but better not lose an eye.
It's a UV pulsed laser, it most definitely needs eye protection (and possibly hand protection as well, but I don't work with UV lasers because the required safety precautions are insanely difficult/costly to fulfill here)
Back in the 90s, a guy who worked at an air force base,gave me a laser that he found in storage. some guy had made it in the 70s or 80s in his spare time from a magazine article, but one of my favorite movies was My Science Project. So I had a very high opinion of it. In this video,I recognize a lot of the parts. Mine has an watched board for the base, & plans are ancient photocopies.
So cool! I did similar a few decades back but I made my pump with plexiglass and pumped it down with a fridge pump, soooo loud and yeah, I did burn my eyes a little being in the room without full circumference sheilding on the glasses. Lesson kids: ALWAYS wear eye protection that coverover your eyes all the way around to the skin when playing with lasers. What you can't see WILL blind you if you are careless. Loved the vid, subscribed.
Quick reminder that 20-ish years ago nearly everyone had a 20,000 volt flyback transformer just a few feet away from them protected from touch by just a few millimeters of plastic. I am of course talking about CRT televisions and in case you are wondering YES people did die poking around in them with screwdrivers.
I am from Northern California, but this is the year that I am out of the state for my pre-doctoral internship… I am so sad that I’m going to miss seeing all of you when you would all be so close to my home. I hope you do this again!
Thank you so much for posting the dye concentrations in sensible units! I'm making a rhodamine b dye laser with a SHG-YAG and the main unknown I've had trouble nailing down was how much dye to use.
In the next few months I will have a house with enough space to do projects again and I hope I learn to do biohackery even a fraction as insanely as you there. :)
As an electronics engineer, I can say you garbage laser has everything an electronics device shouldn't have : very high voltage with high current, exposed metal parts, no isolation whatsoever, massive EM emissions, bad contacts, sharp angles, etc... And yet really want to build one. Impressive work
I spoke to the scientist that created Glo- fish and ive been wanting to learn more about different proteins from jellyfish and other specimens. I never considered they could be made into a laser 😂. Love this channel already.
So happy to see a fellow mad scientist on their way to making the potentially possible, possible. I haven’t taken any steps yet but I hope to in the next couple years
For the first 3+ minutes all I've been wondering about how uv tends to kill cells, good thing he did take that into consideration for the video explanation
I've wanted to do this ever since I saw Styropyro's video where he used a laser tattoo remover to pump a dye laser and wondered whether you could pump a dye laser using a home-built TEA laser. I tried and failed to build a TEA laser for this multiple times(I had horrible luck and little engineering skill). I ended up giving up, partially because this video came out at around the time I was planning out my own TH-cam video on building a dye laser and I didn't want to make a video that had already been made, and partially because I had wasted every box of tinfoil in the house due to accidentally wrinkling it too much for it to work or accidentally burning through it. To be honest, I naively assumed that getting the dye to lase would be easy once I got the TEA laser working, and I'm kind of glad this video saved me from wasting hundreds of hours trying to make a dye laser work while barely being able to afford proper mirror mounts, let alone a 3d printer. However, in my quest to build a dye laser as a broke teenage wannabe science TH-camr, I have made friends with a lot of awesome science TH-camrs and my own channel has grown from nothing to hundreds of subscribers. I am planning to try again eventually once I can save up enough money to buy actual optics and fix the broken 3d printer I was recently given by a friend, and I would like to thank you for making this video, as it's going to be massively helpful for my next attempt.
We have the same "lucky reptile" meatcubators in the lab i work in. Some of them after about half a year developed an awful fault: they would crank their setpoint to 60C, and cook the content. It was a real challenge to find the cause of the issue. It seems to be caused by leds in the display not behaving as good diodes, causing false button press detections (because pins are shared).
Something I am slowly becoming obsessed with is a true blue feathered chicken. I think I have found the right person. I will probably send you an email about this project for advice in the next year when I get more set up for this experiment!
watching stuff like this makes me realize how much of my life i have let go by without having my childhood want to build things and experiment ..life isn't over tho.. inspiring :)
Wow! Thank you so much for the information. I am now a subscriber and look forward to personally going far down the laser world rabbit hole. Again, thank you so much... GL
F***k sake.... Damn.. okay, i just feel like yeah, you're kind of a crazy scientist, and all i just have seen is hyper-mega-ultra awesome. Basically brought a ton of new info for me in like 2-3 first minutes, which i'm happy about. And this speciality is unusual ah... subscribing here with no hesitation, and just hope that'd lead to ton of innovative discoveries. with what i study as an applied to magistracy, i'm glad i found something i can really claim as science. Not in our institution, unfortunately, but at least here. Good luck and tons of patience to you man, thank you for you work! looking forward for further vids, gone checking other stuff you do here
The 2 funniest moments in this video for me was at around 4 minutes in the narrator was using what are now common terms (Genetic manipulation, building them by design, etc) that were all but science fiction until my 30s!!! And "This is for Educational purposes only" line and then shows exactly how to build everything!!!
This is interesting. I worked with a 1-watt CW laser that used Rhodamine 6G. My first thought about using a more complex molecule is that more of the light will be trapped in states that are not optically connected to the ground state. A solution to this for dye lasers is to circulate the dye at a fast rate to keep the dye exposed to the pumping laser "fresh."
It will probably be covered in that next video, but I’m intrigued by the Dye from E. Coli at that Makerspace scale. Dye lasers are cool and all, but applying that to other uses of florescent dye (Microscopy Stains, Art, Non-Destructive Testing, etc) would be neat, especially with like how you said it being cheap.
I’m intrigued as to your methodology. I’m guessing column chromotography? If I remember correctly that is what the company Gabriel Lienca mentioned that makes blue dye from algae uses. Although if you can extract it with something less intense that is always a food thing. (Although i would love to have you design an Open Source Continuous HPLC System…)
Stuff like this is what motivates people to become biotechnologists. Making whatever you want, playing with organisms and modern technology creating something that sounds like magic
True, this channel is the main reason I'm pursuing genetics rn
Absolutely, I chose to do my biotech bachelor's because I wanted to know how fluorescent cats worked and I'm now halfway through my masters
yup same here
And the amazing thing is that all of this can actually be what your job is like, depending on the specifics. For example, a bio laser could be used in experimental cancer treatment, advanced brain scanning technology, indicators that literally point to the location, and more.
Im a computing scientist and I'm disappointed we don't use more lasers.
Where are my optoelectronics ?
I mean, I know about photolithography which is basically how every chip is made ever.
Still , want to play with lasers .
the fact your laser made of "trash" worked better means the fates have decided you are a garbage bender now. you made too many amazing things from scrap that it's your medium now. o7 keep up the amazing work
Welp if it means reduce, reuse, recycle then it's probably for the best right?
Gomi no Sensei!
I cracked! It shouldn't be that funny "water, earth, fire, air,... garbage!"
i had a mental flash of doc from back to the future digging through the garbage for fuel too !!.
Toph approves.
Thanks for this. Almost 40 years ago in 8th grade I had the assignment to 'design' a biological creature. I had been obsessed with dye lasers from a library book on how to make your own (I later dug into research and Amateur Scientist articles), and imagined a creature with a biolaser built in, with a flash lamp pumped with the biology of an electric eel, with fluorescent liquid similar to a jellyfish, biolenses of a squid's eye, and partially reflective material similar to a cat's iris or the skin of some fish. The teacher was very critical and said it was so improbable. But that wasn't the assignment, and I remained convinced all this time that all the components were possible, with occasional flashes of vindication coming out, including this one. I still have the desire to build a dye laser so I will have to pop it a little higher in my project list.
Bro. You're on another level
Finally. Sharks with lasers.
Well boo to that teacher! Sounds like you thought it through very well
@@freelancer917 bad idea! the only reasons sharks haven't taken over the world yet is cuz they haven't got good enough guns yet!
Things will get interesting once we get to the stage where we can do fully simulated generative DNA design and program in biological components as needed. I think we have the tools to start working on this now.
I have worked with dye lasers and want to share some insights:
- You do not need UV light to pump Rhodamine 6G. The frequency-doubled output of a Nd:YAG laser (532 nm) is typically used and is much more efficient since the quantum defect to the emission wavelengths is small.
- A pumped stagnant dye solution will quickly get trapped in states that are unresponsive to pump light. The dye solution is therefore typically circulated so that the dye molecules have time to return to the ground state before being pumped again.
- The optimal dye concentration depends on the length of the cuvette with longer cuvettes needing less concentration. The concentration also shifts the tuning range of the dye with higher concentrations shifting to higher wavelengths.
- The surfaces of the cuvette can themselves act like a laser resonator, resulting in an unwanted beam. The cuvette is typically vertically tilted to steer that beam away from the intentional one.
- The positioning of the cylindrical focal lens is quite important as you have correctly identified. However, tilting the lens like that results in aberrations that lead to uneven illumination. A better solution would be to move the whole lens closer or farther away from the cuvette. Where the lens needs to be rotated is around the axis set by the pump beam. This tilt angles the focal line. You can either align the focal line with the laser resonator for maximum conversion efficiency or tilt it slightly away from it to limit the amount of amplified spontaneous emission that travels along with the dye laser beam.
- Rhodamine 6G is indeed a nice dye.
Do you need to lock the 532 or just a simple output of a laser pointer (the ones sold on e.g. AliExpress is enough)? What are the powers for it you think? The techniques he used in the video are for pulsed lasers afaik, would be more interested in CW
i absolutely love how you discurage from building the laser but still provide all the details "just in case"
The 'technicalities' of the desire to transfer knowledge, while at the same time avoiding a channel/video takedown because of 'Karens' (or atmospherically geometrically frozen water).
@@threeMetreJim ah yes hes saying to not build the lethal insta kill/blind machine cuz hes afraid of yt taking him down
plus his mum said ,' don't do that ' many times ,so he is naturally programmed to have to try it , knows we are too ,human nature ..ever since Eve actually ,When the Big Boss ,said don't eat that trees fruit it will kill you , what did she jolly well go and do !!??.
You can't stop people from doing dangerous things. You can show them how to minimize the risks
"Don't actually try this. Here's the circuit diagram of the laser..." - The Thought Emporium
Neat stuff! The rail laser is crazy, never seen anything like it.
It is almost surprising to me that you have not yet touched on lasers yet. Then again, that might be a job for Tech Ingredients.
Nice to see you here!
Last thing you'll ever see.
seeing something that generates that much power with essentially no insulation is pretty terrifying lol
Styropyro and The Plasma Channel also made their own versions of that laser. Both just as terrifying.
Styro
watch?v=1psxE4NlECQ
PC
watch?v=YFkUOPl0tUw
Styropyro made it a while ago
I came to a dead stop at “huge collection of genetically engineered E. coli.”
To a guy with thousands of books, parts for obsolete computer architectures, and a ton of other random stuff “too good to throw away,” that seems weird.
Yet you did something with it. I’m envious.
Literally synthesized molecules in an abandoned farm house for 2 years trying to make one of these dye lasers.
And the meat laser? Way farther than i ever could achieve.
I made 3 dyes but i could never get them into lasers. I didnt have ambition, or 3d printing but man. This was literally my dream as a 17 year old.
Thank you for reminding me of the days of gold when chemistry channels like nurdrage and chemplayer and masterminds like styro the like Began to inspire me. You really rock, bro.
Yep, this guy is seriously skilled. I was blown away
You created life?
@@Noconstitutionfordemocrats1 truthfully that not a impressive accomplishment. but I think you misread my comment if that's what you absorbed from it.
@@dontworry4945 So, a whole celebrated book is devoted to the concept of creating life, in which case a monstrosity is born, but when we do it today and answer a thousands-year old question of where do we come from, we're just, like, "meh"?
@@Noconstitutionfordemocrats1 granted, I curse in 6 languages fluently and was raised speaking both UK and US English, somehow I didn't get creating life out of synthesizing molecules.
For fuck's sake, burning hydrogen synthesizes water molecules! That sure as shit isn't life, but absolutely necessary to maintain it.
Of course, synthesizing organic chemicals properly, that's a wee bit more complicated.
How neat that you went over the build process in such detail
But remember - don't try this at home
@@Jackson_Zheng 1984
As one of my optics profs once said: almost everything will lase if you pump it hard enough.
Also, and without getting into the intricacies of building a high end laser, a simple trick to make the laser cavity more stable (which should make it easier to align) is to replace one of the end mirrors with a slightly convex mirror (i.e. with focal length larger than your cavity length).
Yeah, creating a more stable (as in stability polynomial, not necessarily acoustically) cavity is to provide at least *some* form of focusing.
Also for anyone else interested in doing this themselves:
Just because the commercial UV laser was *safer* (electrically) to operate it's STILL A UV LASER which means it basically automatically is a class 3 or higher laser and also carries a higher risk of cancer (I am not entirely sure if this is wavelength dependent, but in all laser safety briefings and classes our magical wavelength is 400nm and everything below that requires *very* hard to get safety gloves and thorough documentation for something like 40 years due to the potential increase in cancer risk if exposed to the beam)
@@reinei1 you're right it's wavelength dependent. UV light is just the right energy level to cause particular mutations in our DNA (two bases covalently bond together), whereas visible light is too long wavelength. I'm guessing high power visible light would just burn a hole in your skin before causing cancer
@@reinei1 and blindness,, gotta make sure those goggles are validated correctly
@@sciencegeek499 which is even more important with dye lasers since you can produce colors that no goggles exist for
@@reinei1 Electrical Interlocking and MAJOR Safety Precautions are for nerds though…
( /j , the people behind this channel are probably doing all that / fine, but yeah especially the diode lasers selling these days really pretend laser safety doesn’t exist)
this brings a whole new meaning to flesh light
Bro made a Scorn gun💀
No, I don't think it does. How do you figure "laser" and "sex toy" are close enough to associate the name of a sex toy with a laser?
@@darrylkinslow5613a meat laser.
a flesh light.
@@darrylkinslow5613You... Really aren't very smart, are you?
@@kadrix732 Yes, I am. I'm smart enough not to associate something like this with a sex toy. You'd have to have a pretty low IQ to make that association.
Dude, if I had known that you needed dyes I would have sent you some of every kind that I have on hand for free. I really need to get up with you on donating some stuff one day. As a chemical distributor, I have tons of dyes, just like these, that you could just have. Take fluorescein for example, I have pounds upon pounds of the pure powder, plus tons of others. If you ever need anything like that, just let me know and I'll gladly send you a ton of each absolutely free. 😃
can you help me make methamphetamine
Thank you all for the encouraging remarks! I will reach out when I find the time to do so and I will try to donate some chemicals and elements to his channel/business to help save him some money and to just help out. Ignore the guy wanting to make drugs. I get comments like this occasionally due to some of the things that I sell and I do not appreciate those types of comments and I report any suspicious people to the feds because I am required to do so by law in order to be able to keep selling such things. Don't want to get a visit? Don't ask me to help you make drugs. Simple.
☹️
@@Shrek_Has_Covid19not gonna lie, that made me snort.
@@Shrek_Has_Covid19Did you actually think doing that was worth it lol
I built one of these when I was 13 not realizing how dangerous they were, I am still surprised and thankful I did not get electrocuted or blinded doing it!
Oh sweet 3D files for a dye laser. I have Been looking for this. Thank you!
"Then I just download the genetic sequence from 'thingiverse' and quickly insert it into e-coli"😂😂😂😂
It really feels like stuff is ramping up this year! Looks like the infrastructure work over the last few years is really paying off.
I can only imagine what your ultimate goal could be with all of this, I suspect that a lot of this stuff has already been played with in the big labs, but the fact that you are doing it here, just proves that ingenuity and the masses can prevail. Best to you, Ken.
Laser eyes
This video really takes me back about 35 years, when a friend and I puttered around with both N2 lasers and fluorescent dyes in our off time. Something that's not mentioned but should be kept in mind - Rhodamine 6G will stain *everything* you get it on, so aprons or other cover should also be worn in addition to PPE for the risks that will affect your health and not just your clothes.
"I've done so much with so little for so long I can now do anything with nothing"
I saw/read this many decades ago and thought I'd share in case you haven't read/heard. Very appropriate for you imo.
Something I tell my kids and others, "There is nothing hard, just things you haven't learned yet"
Enjoyed very much, be safe and hf learning.
Downright kaleidoscopic rush of a video, and all of it was actual genuine examples of what amazing, beguiling thing you're working on! _No wonder_ I'm subscribed to you guys.
You single-handedly convinced me to switch from physics to biology back in 2021. Mad science is awesome!
I graduated in physics, but I have a general understanding of most fields of science (nerd with adhd)... Anyway, genetic stuff is literally magic to me.
I'm still not 100% convinced that bio engineers aren't just wizards.
biology is amazing but not enough for me to ever switch from physics lol
I'm a laser physicist by trade and this is a fantastic video. Most of it is familiar, but i'm very impressed by how well you synthesize all these different topics and condense them without necessarily reducing them to an inaccurate form. Specifically, the topic of grating based mode-matching is awesome and a bit of a pain to explain well, but you managed to do it in less than 30s!
One critique is that the TEA doesnt work via stimulated emission, but rather ASE (amplified spontaneous emission). This also means that although it is fairly temporally coherent, it isn't perfectly so which is why it only runs on a pulsed source.
“And I’m the only bio-engineer on TH-cam crazy enough to try.
That, in a nutshell, is why I subscribed in the first place 😅
great to see ur lab growing well! sputtering, meatcubator, lasing set-ups, so cool!
Modern science truly is amazing, can't wait for the meat laser.
"I maintain a huge collection of genetically engineered E Coli" belongs in an xkcd somewhere
Thanks to StyroPyro I immediately recognized the TEA laser and am immediately scared.
Also that feeling of building it all proper like and it works worse than the prototype is so much feels.
This gives me the same vibes as writing my engineering honours project, you've clearly done tonnes of amazing work and have absolutely no time to show us the immense amount of work you had to do to get to where you are. Very interesting and exciting video, loved it!!
i like that you used this one paragraph compliment to… brag about yourself. like your first sentence added exactly nada to the comment, and makes you look socially iffy
@@iwanttwoscoops appreciate your feedback, champ!
@@matthewphillips9083 gotchu dad
No period on the T.E.A acronym graphic. This isn't critical feedback, just a very relatable moment. Addicted to your channel now. Thank you!
"I wouldn't recommend you building this, but here is exactly how to build it" 😂
This man talks about meat lasers like they're the one piece or something.
Your nitrogen laser is a beautiful build! Far superior to mine I must say. Great video!
Thanks man! Your video was really great too!
@@thethoughtemporium
Please do some experiments with a Kozyrev Mirror.
I would really like to know the truth in what you experience.
Apparently according to the team who work with it and others it seems to really concentrate time and the experience is horrifying to some.
My lord this is an incredibly well crafted TH-cam video.
your mad scientist projects have gotten so ambitious. this is some wild stuff man, can't wait to see how you make it work.
I can't begin to imagine how many watchlists you're on, but I appreciate the work
Thinking quickly, Dave crafted a laser out of cells, a dye, and a laser!
I discovered Les's Lab few days ago, and what he does is very impressive, but also shows how some of these things are not as complex as they might initially look. I knew of your channel for years, but this is a great showcase of lasers. The dye lasers are cool. It is also mind blowing that anybody can just engineer DNA of a bacteria with just few clicks in a browser. Wow.
2:30 So basically bio engineers copy code just as developers do with stack overflow and GitHub... biology is an open-source repo, gotcha.
Thinking quickly, Dave constructs a homemade -megaphone- laser, using only some string, a squirrel, and a -megaphone- laser.
Impressive work! But don't forget to protect your skin from UV, good sunscreen is a good option if you dont want cover yourself up all the time
Damn that's amazing, those colors are so beautiful. I miss my days in the spectrometry lab. Looking forward to when you can sear your initials into meat with... umm... meat. :)
It's weird seeing a video on such a sketchy laser machine and not be reminded how it'll delete my retina if i even think about looking at the the spot on the wall
"Wow! This thing is so sketchy that...... etc etc"
MEAT LASER!!! ok we are getting into supervillain territory. . . and i am 100% here for it
Bro I didn’t even know you could do almost any of what you talked about, that’s super cool, like ordering custom dna things that grow with your desired codes or whatever you called it, wild man, keep it up
"We really don't recommend you try to build one, seriously it's super dangerous and could easily kill you in an instant. So anyway, here's exactly how you could build one."
This is making me think about that silver nanoprism video from applied science a while back, and that’s one I’ve gone back to quite a few times. Great video, love this stuff as always
For the love of god please please no one ever break this man's heart.
If he turned evil we are doomed!
8:34 Just look at that coronal discharge! Going from the sharp corners as they should! Beautiful!
Something I don't see anywhere which should be shouted from the rooftops. WHERE EYE PROTECTION WITH LASERS.
Not sure if this is the type that needs it if it's a special case, but better not lose an eye.
It's a UV pulsed laser, it most definitely needs eye protection (and possibly hand protection as well, but I don't work with UV lasers because the required safety precautions are insanely difficult/costly to fulfill here)
can't wait for the video titled "we gave fireflies an upgrade"
Colab with styropyro?
Back in the 90s, a guy who worked at an air force base,gave me a laser that he found in storage. some guy had made it in the 70s or 80s in his spare time from a magazine article, but one of my favorite movies was My Science Project. So I had a very high opinion of it. In this video,I recognize a lot of the parts. Mine has an watched board for the base, & plans are ancient photocopies.
WHAAAT? Dude, love the synergy between disciplines and perceived simplicity you show here. That's beautiful science, I love it. Thank you.
What a refreshing beacon of hope, rigor, and whimsy.
You mean "bacon" of hope, I think.😅
This is a crazy project. But your work is so amazing and inspiring. Good job bro!
"oh your He-Ne laser is stabilized with iodine? Mine's stabilized with a 16oz filet mignon"
"I want to build a meat laser"
what a way to start a video
So cool! I did similar a few decades back but I made my pump with plexiglass and pumped it down with a fridge pump, soooo loud and yeah, I did burn my eyes a little being in the room without full circumference sheilding on the glasses.
Lesson kids: ALWAYS wear eye protection that coverover your eyes all the way around to the skin when playing with lasers.
What you can't see WILL blind you if you are careless.
Loved the vid, subscribed.
Quick reminder that 20-ish years ago nearly everyone had a 20,000 volt flyback transformer just a few feet away from them protected from touch by just a few millimeters of plastic. I am of course talking about CRT televisions and in case you are wondering YES people did die poking around in them with screwdrivers.
Not what I was expecting to think about today but here it is and it’s fantastic
And this is the person is gonna restart the zombie apocalypse
The amount of information we get here for free boggles my mind every time.... Can't wait to see what's coming up next in the future.
I am from Northern California, but this is the year that I am out of the state for my pre-doctoral internship… I am so sad that I’m going to miss seeing all of you when you would all be so close to my home. I hope you do this again!
We missed your videos and mad scientist inspiration!
8:33 love the micro blue antennas out of the corners when it's not arcing
You guys have the secret sauce for massive technology advances - cross pollination of these vastly different disciplines. Massive love from Beryl CNC!
I wish styropyro helped you out with this, would have been the colab of the century
He has (finally) gone completely insane.
Good
keep building from scratch I love learning form scratch. Amazing stuff!
I used to work in laser manufacturing and i sure do love ThorLabs. They are so fun for an industrial supply company.
Am very pleasantly surprised you made a video on rhodamine 6G as I’ve been looking at it for a while now
Thank you so much for posting the dye concentrations in sensible units! I'm making a rhodamine b dye laser with a SHG-YAG and the main unknown I've had trouble nailing down was how much dye to use.
In the next few months I will have a house with enough space to do projects again and I hope I learn to do biohackery even a fraction as insanely as you there. :)
Man, I love this guy's videos. I wish I grew up nextdoor.
As an electronics engineer, I can say you garbage laser has everything an electronics device shouldn't have : very high voltage with high current, exposed metal parts, no isolation whatsoever, massive EM emissions, bad contacts, sharp angles, etc... And yet really want to build one. Impressive work
Thank you for maintaining this channel. Your videos give me hope for humanity, lol.
I spoke to the scientist that created Glo- fish and ive been wanting to learn more about different proteins from jellyfish and other specimens. I never considered they could be made into a laser 😂. Love this channel already.
Lasers of diverse colors is beautiful and exciting
So happy to see a fellow mad scientist on their way to making the potentially possible, possible. I haven’t taken any steps yet but I hope to in the next couple years
OMG! This is the thing! I suggested this back when the pandemic started!
I believe this man's work will bring forth some good discoveries one day
I've been waiting many years for more work to be done on bio lasers. Thank you sir.
For the first 3+ minutes all I've been wondering about how uv tends to kill cells, good thing he did take that into consideration for the video explanation
I've wanted to do this ever since I saw Styropyro's video where he used a laser tattoo remover to pump a dye laser and wondered whether you could pump a dye laser using a home-built TEA laser. I tried and failed to build a TEA laser for this multiple times(I had horrible luck and little engineering skill). I ended up giving up, partially because this video came out at around the time I was planning out my own TH-cam video on building a dye laser and I didn't want to make a video that had already been made, and partially because I had wasted every box of tinfoil in the house due to accidentally wrinkling it too much for it to work or accidentally burning through it. To be honest, I naively assumed that getting the dye to lase would be easy once I got the TEA laser working, and I'm kind of glad this video saved me from wasting hundreds of hours trying to make a dye laser work while barely being able to afford proper mirror mounts, let alone a 3d printer. However, in my quest to build a dye laser as a broke teenage wannabe science TH-camr, I have made friends with a lot of awesome science TH-camrs and my own channel has grown from nothing to hundreds of subscribers. I am planning to try again eventually once I can save up enough money to buy actual optics and fix the broken 3d printer I was recently given by a friend, and I would like to thank you for making this video, as it's going to be massively helpful for my next attempt.
We have the same "lucky reptile" meatcubators in the lab i work in. Some of them after about half a year developed an awful fault: they would crank their setpoint to 60C, and cook the content. It was a real challenge to find the cause of the issue. It seems to be caused by leds in the display not behaving as good diodes, causing false button press detections (because pins are shared).
Something I am slowly becoming obsessed with is a true blue feathered chicken.
I think I have found the right person.
I will probably send you an email about this project for advice in the next year when I get more set up for this experiment!
First video I've seen from you. Fascinating stuff. Subbed.
It so nice to see such high quality DIY content.
I'm jealous on so many level!!!
Love from Montréal
oh my gawd a 16 minute video from TE, today is a good day
I’m definitely not going to do this, but if I wanted to this video would be a godsend. Well done
just brilliant, mad science and brilliant information delivery thanks ! keep making videos :)
watching stuff like this makes me realize how much of my life i have let go by without having my childhood want to build things and experiment ..life isn't over tho.. inspiring :)
Wow, this is good. I have never thought living cells could be used for stimulated emission!
Now I have gotta build my own!
Wow! Thank you so much for the information. I am now a subscriber and look forward to personally going far down the laser world rabbit hole. Again, thank you so much... GL
F***k sake.... Damn..
okay, i just feel like yeah, you're kind of a crazy scientist, and all i just have seen is hyper-mega-ultra awesome.
Basically brought a ton of new info for me in like 2-3 first minutes, which i'm happy about. And this speciality is unusual ah... subscribing here with no hesitation, and just hope that'd lead to ton of innovative discoveries.
with what i study as an applied to magistracy, i'm glad i found something i can really claim as science. Not in our institution, unfortunately, but at least here.
Good luck and tons of patience to you man, thank you for you work! looking forward for further vids, gone checking other stuff you do here
That laser looks like it might double as a piece of broadband RF jamming equipment.
The 2 funniest moments in this video for me was at around 4 minutes in the narrator was using what are now common terms (Genetic manipulation, building them by design, etc) that were all but science fiction until my 30s!!! And "This is for Educational purposes only" line and then shows exactly how to build everything!!!
Imagine a Jedi uses a rat or his hand to power his lightsaber
This is interesting. I worked with a 1-watt CW laser that used Rhodamine 6G. My first thought about using a more complex molecule is that more of the light will be trapped in states that are not optically connected to the ground state. A solution to this for dye lasers is to circulate the dye at a fast rate to keep the dye exposed to the pumping laser "fresh."
It will probably be covered in that next video, but I’m intrigued by the Dye from E. Coli at that Makerspace scale.
Dye lasers are cool and all, but applying that to other uses of florescent dye (Microscopy Stains, Art, Non-Destructive Testing, etc) would be neat, especially with like how you said it being cheap.
I’m intrigued as to your methodology. I’m guessing column chromotography? If I remember correctly that is what the company Gabriel Lienca mentioned that makes blue dye from algae uses.
Although if you can extract it with something less intense that is always a food thing.
(Although i would love to have you design an Open Source Continuous HPLC System…)
The only complaint I have with this channel, is the upload frequency. These videos are so interesting.