David Baker (U. Washington / HHMI) Part 2: Design of New Protein Functions
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- เผยแพร่เมื่อ 8 มิ.ย. 2024
- www.ibiology.org/biochemistry...
Lecture Overview:
Baker begins his talk by describing two reciprocal research problems. The first is how to predict the 3 dimensional structure of a protein from a specific amino acid sequence, while the second is how to determine the amino acid sequence that will generate a new protein designed to have a specific structure. Baker’s lab is addressing the second of these challenges by developing computer programs (such as Rosetta@Home) that calculate the lowest energy, or most likely, structures for differently folded amino acid sequences. Baker explains how his lab can design a new protein structure, not found in nature, and using the computer programs they have developed, determine the amino acid structure. It is then possible to back translate to the DNA sequence and synthesize the gene that can then by used to make the protein. When the structures of these synthesized proteins are determined by crystallography and compared to the predicted structures of the designed proteins, they are found to overlap very closely demonstrating that the protein design algorithms work well.
In the second of his talks, Baker tells us how his lab has moved beyond designing new protein structures to designing new protein functions. The first example he describes is the development of an inhibitor of the influenza virus. Baker’s lab designed a protein structure that fits into a highly conserved region of the hemagglutinin protein found on the surface of influenza. Preliminary lab data suggests that this designed protein protects mice from infection with the flu virus. Baker also describes experiments in which proteins were designed to fit together and build multicomponent materials such as nanocages, nanolayers and nanowires.
Speaker Bio:
David Baker received a BA in Biology from Harvard University and a PhD in Biochemistry from the University of California, Berkeley. Currently, Baker is the Head of the Institute for Protein Design and a Professor of Biochemistry at the University of Washington, and a Howard Hughes Medical Institute Investigator. His research utilizes both experimental and computational methods to study the design of protein structures, and the mechanisms of protein folding, protein-protein and protein-small molecule interactions.
Baker’s lab developed the crowd-sourced protein folding design programs Rosetta@home and Foldit. Learn more about these programs in Baker’s iBioMagazine talk and at his lab webpage www.bakerlab.org/static/ .
Baker has won numerous awards for his work including the Raymond and Beverly Sackler International Prize in Biophysics in 2008. Baker is a member of the National Academy of Sciences and the American Academy of Sciences. - วิทยาศาสตร์และเทคโนโลยี
It's been 4 years that I'm studying mechanical engineering,but now I've fallen in love with proteins and dna and etc,
It’s the same thing, only on a smaller scale.
This guy is so cool!! Always appreciate talented researchers who know how to communicate their work clearly!
this so fucking cool, i wonder how much this topic has advanced since then
Wow this is a fantastic lecture. Everyone should see this.
LOL love the climbing analogy - am one myself and loved my days as a protein folding functional scientist that went into redox systems biology
So fascinating! Thank you for making this lecture!
Incredible!
Very interesting challenge
The 3d shape on 19th min is actually a dodecahedron, not icosahedron :)
I really want to create new proteins xD
Wow!!! I am just truly fascinated by the amount of work and research that has been invested here! Thank you for the great lecture!
Amazing
Hi, I am Pratibha from IITH. I am curious to learn nano cage protein designing. Can you share some information about this.
Let's assume i designed a protein with alphafold2 that recognizes a specific ligand, how to check it if it's working?
Genius David Baker xoxo .. I wish to work with him
5 years later doctors keep telling me there is no treatment for flu...
From concept to commercial pharmaceutical can take 20 years. (And before you say, "what about the coronavirus vaccine?", that had billions of dollars poured into it, and was built on research that had been happening for years before.)
Wow!
Wowwwww
Did they research this new materials for it electrical, thermostability, chemical activity, optical and mechanical characteristics?
Maybe it can be usefull for some nonbiological application?
For example lubricants, fiber, flexible electronic, electrodes for implants, micromechanical systems and so on. Looks like potentially very useful things even today. Give the samples to physics))
Designing materials with certain physical properties would probably be even more difficult I'm guessing--although protein structure is already insane.
'Viral epidemics' WHAT DID YOU KNOW haha
1:54 well..................
7:30 What if you designed a single protein that docks and goes through a conformational change once docked that creates the same dock on it. The new dock would attract another of the same protein which would go through the same conformational change creating another dock. This would reduce the number of proteins required to one while allowing you to modify the dock and proto-dock while keeping the mechanism binding the two intact.
23:30 “Not for bad purposes…”
If only intent were enough.
23:00 How about a nanocage that encloses an E. Coli bacteria modified to create insulin? The cage would allow resources to reach the bacteria cells, but would restrict the space in which the E. Coli can multiply within.