Flow Cytometry - 2 | Hematopoiesis & CD Markers - In Just 10 MINUTES !!!!

This video is the second part of the basics of flow cytometry which covers hematopoiesis and CD Markers. Hematopoiesis and CD markers form the backbone of Flow Cytometry. CD markers is a very high yielding topic . Please do not enter exam hall without being sure that you know CD markers very well. After understanding the principles of a flow cytometry machine, we next move to the uses of flow cytometry in various hematological disorders. We will first understand normal hematopoiesis in the bone marrow. We will also understand about CD markers that form the basis of flow cytometry are used to label and identify different types of cells. Hematopoiesis is the process of creating new blood cells in the body. Each type of cell in blood has its own unique signature, whether it is a blast, lymphocyte, monocyte, neutrophil, or RBC. This signature is defined by CD markers. No matter how many times we read about CD markers and hematopoiesis, we tend to forget it. So, in this video, we will learn to apply CD markers in a practical way so that we can remember them
If we know how normal hematopoietic cells behave in flow cytometry plots, then only we can identify abnormal patterns in different types of leukemias.
the first video on flow cytometry:- • Flowcytometry Basics -...
the second video on flow cytometry:- • Flow Cytometry - 2 | H...
the third video on flow cytometry:- • Flow cytometry -3 | A...
the fourth video on flow cytometry:- • Flow Cytometry - 4 | C...
Chapters
00:00 Introduction to Hematopoiesis and CD markers
01:43 What are CD markers?
02:53 Uses of CD markers in Flow Cytometry
03:44 Table of CD markers
04:53 Normal Hematopoiesis and immunophenotypic changes
06:04 Hematopoietic stem cell
06:29 Myeloid Lineage hematopoiesis / Granulopoiesis
07:51 Monocytic differentiation
09:20 Megakaryopoiesis
09:57 Erythropoiesis
10:54 Next video teaser !!!
Learning about CD markers is the key to understanding and interpretation of flow cytometry.
Hematopoiesis is the process of creating new blood cells in the body. All blood cells start off as hematopoietic stem cells and then specialize (differentiate) into myeloid cells (erythrocytes, megakaryocytes, monocytes, neutrophils, basophils, or eosinophils) or lymphoid cells (T-lymphocytes and B-lymphocytes).
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[@greendestinyph]

Nice lecture. Thank you!

[@drpathlogist2058]

Excellent description of cd markers in flow cytometry !! Keep up the good work

[@pathogenesis4363]

Thank you!!!

[@shotarozee]

Great lecture!! I can finally understand flow cytometry. When will next part uploaded. Can’t wait to watch it!

[@pathogenesis4363]

Thankyou ! Working on it . Should be online in 3-4 days

[@koustubhshekar4639]

Great work

[@pathogenesis4363]

Thank you koustubh !!!

[@meghazacharia4857]

Good video.Well done

[@pathogenesis4363]

Glad you liked it ☺️ stay tuned for next video on cd markers in lymphoid series where we will be covering origin of lymphoid leukemias and lymphomas

[@isaacmccool7312]

Excellent lecture!!!

[@pathogenesis4363]

Thank you

[@pathogenesis4363]

Thank you

[@setzz69]

brilliant

[@pathogenesis4363]

Thank you

[@deeksha244s]

Good video and explanation...
Kindly make video on myeloperoxidase and marker differential for AML diagnosis

[@pathogenesis4363]

Sure .. thanks for your feedback

[@pathogenesis4363]

You mean different types of aml ??

[@deeksha244s]

Yes that’s too ...
But a clear picture of myeloperoxidase and as cytoplasmic mpo marker is used to identify via flow cytometry...

[@pathogenesis4363]

Sure .. will be glad to help in whatever students want

[@rahmandr.farzana6927]

Thanks

[@pathogenesis4363]

Glad to know you liked it Dr. Farzana

[@rajeshmeghani3057]

I m devlopd my owen reagent ....but some time clusturing effect plz halp me

[@hitkarshkushwaha2434]

👍👍👍👍👍👍👍👍👍👍👍👍👍👍

[@pathogenesis4363]

Thank you 🙏

[@DeepakSharma-bb6fb]

Bone marrow aspirate sent for flow cytometric immunophenotyping analysis is hemodiluted and shows ~ 2.3% CD45 dim+ blasts which are CD13+, CD33+, CD34+, CD117+.
Advice:- Correlation with bone marrow biopsy for exact blasts percentage
what is the meaning of above report ??

[@pathogenesis4363]

It means the bone marrow shows 2.3 prrcent myeloblasts . Since less than 5 percent blasts are normal in a bone marrow , a conclusive opinion has not been given in the report

[@pathogenesis4363]

Is it an undiagnosed case ?

[@DeepakSharma-bb6fb]

@@pathogenesis4363sir TLC are increase

[@DeepakSharma-bb6fb]

@@pathogenesis4363sir is it show blood cancer or not ???

[@DeepakSharma-bb6fb]

@@pathogenesis4363 karyotyping for hematological malingnacies report is normal