PPE is missing. Also, you need to keep the dish lid over the dish. That prevents contamination or infection. You literally picked up the entire thing and held it wide open for the bacteria to spread.
Wearing gloves while using a bunzen flame isn't recommended because you can accidently pass your hand through the flame leading to the gloves to melt into your hand. I would recommend wearing googles when you are dealing with acids and bases (during buffer preparation for example) and when dealing with pathogenic microorganisms.
Streak lines need to intersect, so that you can drag colonies from the first streaks into the new ones. Then you sterilize your loop again, and drag a little less from the second set of lines. Then sterilize again, and drag only individual bacteria (single isolated colonies) to the final lines. You don't pick up more material between lines, you use the previous lines to create the new ones. The idea, is that you pick up less and less with each set of lines, so your final lines have individual colonies of bacteria, or whatever you are culturing. That is why the first sets of lines are called "tertiary lines" as you don't use them later. When you want to sample from your agar plate, you will use the isolated colonies from the final streaks.
No. You let the loop cool for a couple seconds. You can test it by gently laying it on the agar and watch for sizzling. No sizzling = cool enough. You heat it up first to kill the unwanted bacteria.
no, not really. She never makes contact with any of the samples, using her hands. She only uses her sterile loop. The only real reason to cover one's hands in this case would be to stop her from contaminating the cultures; however, as long as she has cleaned her hands and then used a sanitizer it will be fine. As well, this is pretty much just a demonstration lab, for people to learn how to use aseptic techniques with the loop and proper use of reduction with applying streaks to a plate. I am sure, that if doing practical lab work, she would likely have a mask, hair cover, gloves, and would likely do it all inside of a Flow Hood.
some labs prefer no gloves to prevent accidentally melting them onto your hands, which is more of a hazard than just singing yourself a bit. obviously this is a trade off, depending on which organism you are working with. if you are working with a more dangerous organism you would not generally be working on the bench with a flame, rather in a laminar flow where you can use gloves etc.
It's more of a hazard to melt the glove onto your skin. Also, this probably isn't O157:H7 strain of E.Coli so there's no risk of huge dangerous outbreak.
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PPE is missing. Also, you need to keep the dish lid over the dish. That prevents contamination or infection. You literally picked up the entire thing and held it wide open for the bacteria to spread.
That's what I was thinking, though the other information is very good, I think.
do you know videos that do that in youtube? i've seen all of them lift them all the way. Thank you!
Best demonstration I’ve seen. Thanks :)
Great job... your safety is key that's why we have to use personal protective equipment while handling laboratory specimens.
This is the best 🙃👍👍vidios on you tube wrt streaking .
Keep it up....😇😇 love from india.
You are a good teacher. Thank you for this video.
We're going streaking!!
Tahnk you ! very helpfull , very carefull explanation , slow and easy !
Thankyou! Really clear explained. :)
how do you determine the "safe area" to do your transfer in around the flame ? ie I want to transfer agr to agar where do I place my plates?
Super helpful, thank you so much! :)
good presentation.
Great video thank you so much but where is you PPEs (personal projective equipments)? Gloves, googles etc..?
Wearing gloves while using a bunzen flame isn't recommended because you can accidently pass your hand through the flame leading to the gloves to melt into your hand.
I would recommend wearing googles when you are dealing with acids and bases (during buffer preparation for example) and when dealing with pathogenic microorganisms.
thanks hgk this homework got me fucked up n you cleared everything up for me 💞
Why must the streak lines intersect?
Streak lines need to intersect, so that you can drag colonies from the first streaks into the new ones. Then you sterilize your loop again, and drag a little less from the second set of lines. Then sterilize again, and drag only individual bacteria (single isolated colonies) to the final lines.
You don't pick up more material between lines, you use the previous lines to create the new ones. The idea, is that you pick up less and less with each set of lines, so your final lines have individual colonies of bacteria, or whatever you are culturing.
That is why the first sets of lines are called "tertiary lines" as you don't use them later.
When you want to sample from your agar plate, you will use the isolated colonies from the final streaks.
wait when she heats it up doesn't it also kill the ecoli bacteria on it too?
no if you ,cool loop
No. You let the loop cool for a couple seconds. You can test it by gently laying it on the agar and watch for sizzling. No sizzling = cool enough.
You heat it up first to kill the unwanted bacteria.
How can I make total fungal count in The presence of mucor ??
It makes it very difficult 😔
here form microbiology class
🍸🍸👍👍💛💛💛
80% of the views are from 2020
who else high af rn
Why are you doing this without gloves? Isn't that unsafety?
yo momma
no, not really. She never makes contact with any of the samples, using her hands. She only uses her sterile loop. The only real reason to cover one's hands in this case would be to stop her from contaminating the cultures; however, as long as she has cleaned her hands and then used a sanitizer it will be fine.
As well, this is pretty much just a demonstration lab, for people to learn how to use aseptic techniques with the loop and proper use of reduction with applying streaks to a plate.
I am sure, that if doing practical lab work, she would likely have a mask, hair cover, gloves, and would likely do it all inside of a Flow Hood.
even this is just demonstration, but she is handle with bacteria, the best way should always wearing the glove for your protection of yourself.
some labs prefer no gloves to prevent accidentally melting them onto your hands, which is more of a hazard than just singing yourself a bit. obviously this is a trade off, depending on which organism you are working with. if you are working with a more dangerous organism you would not generally be working on the bench with a flame, rather in a laminar flow where you can use gloves etc.
It's more of a hazard to melt the glove onto your skin. Also, this probably isn't O157:H7 strain of E.Coli so there's no risk of huge dangerous outbreak.
ok so there is just a few of mistakes here. lol
why talking will that not introduce bacteria?
the torch is taking care of all of that
Indeed, you shouldn't talk in front of your open plate or instrument.
clown