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TISSUE CULTURE UPDATE 2023
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- เผยแพร่เมื่อ 30 เม.ย. 2023
- TISSUE CULTURE UPDATE 2023
I began my tissue culture back in 2022 and I think it is time to do an update since we are now in 2023. Hope I was able to answer some of your questions - feel free to drop a comment down below if I was not able to answer your questions in this video.
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Don't confuse genotype with phenotype! A plant that's genetically variegated can make a few leaves with more chlorophyll if the total plant overall needs more photosynthesis. Once the plant has more leaves and green stems, it won't need to max out the chlorophyll content of the leaves and the variegation trait will show. Likewise most plants will grow a white root but if you expose that root to light for days, even the root will turn green. Another reason to have more chlorophyll is that is acts as a sunscreen until the plant forms enough carotenoids to do some of that job.
Monstera in general really don't like transitioning media. If you do your plant will die halfway before it starts picking up again. So yeah, pick a medium and try to remember your soil blend. I'd try to use the same brand of whatever you used if possible.
I had a pothos that was variegated for five years and I reported it into some very rich homemade compost and it grew a few green leaves just because it had the extra nitrogen and protein. It grew like four green leaves then it grew more variegated leaves after that.
Ah love that for you !
@@leafletco :)
1:33 your disappointment is still a proud insight for you & others so feel confident sharing. Also, I spent most of academic life in plant physiology and cell specimen propagation. I believe you should refer to you babies as “specimen” or “baby whatever” but not really “tissue culture” once moved from monoculture hermetically sealed environment toward other (I.e., seal broken).
11:35 lol…i have been doing it since before pandemic AND your birth. Terminology fuzzy here. Three basic stages: 1) mono culture in hermetically sealed space (your “tissue culture” samples), 2) transfer from 1 and 3) 100% conditioned . The secret is getting carefully from 1 to 2 and fastest arrival to 3. Different techniques and too hard to explain here. Happy to explain but typing fatigue setting-in…perhaps reading fatigue for others too?
I'm so happy for you and your Thai Cons! It's been great following up on your journey. You were so defeated in that one video but it's so worth seeing you succeed at it now! Go Stratum!
I just put my baby TC in stratum and perlite two days ago. So far so good. Fingers crossed!
Aww! Thanks for watching! I truly was defeated but I'm happy they are still growing and thriving :) sending all the positive planty vibes your way!!
Stratum is most definitely the best thing since slice bread. Those Thai's look great!
😂 it sure is!!
I bought a beautifully variegated Billietiae from tc and after 2 new leaves it reverted. About 7 more into it's life now and still no sign of it returning. Grown under Barrina T5 grow lights. I totally get the frustration 😢
Totally agree! All my thai cons in pon since tissue culture acclimation process,for bigger ones I added bigger chunks of pumice/leca/akadama but yeah keeping semihydro, they're really hates this transferring. Learned a hard way 😢😅
Same! I find that if the plant has already really matured it just doesn’t look change at all 🤷🏻♀️
Hey! I'm a bit late... Amazing video^^ What kind of substrate is stratum actually? I'm not native english and struggled a bit when searching for stratum. I only know (and found) it as a volcanic soil underwater substrate for aquariums. It's sometimes sold for terrariums as well. Is it actually that which you're using? Or is it something different?
nice ! so I have a thai con and an albo that almost died on me so I finally was able to reroot them lol they're still sitting in moss I am so nervous but I think I need to place them in soil now.
Lolol so long as you’re giving it nutrients it can stay in there. But if you’re adamant on putting it in soil you can make your soil mix and have some moss in there too so that it can transition easier (moist moss you don’t want to water log it)
@@leafletco awesome thanks ! keep your fingers crossed for me 🤣
@@MyLocsMyPlants I sure will!!
Where are you getting your TC plants?
Is it possible to use led light for growth of plants
Yes you can! All my tissue cultures were under led grow lights
@@leafletco Thanks for reply, how many watt led should I use.
8:40 what vendor in Thailand does smaller volumes? I only know wholesale vendor.
18:26 problem was hobbling your way to “2” in previos comment about stages & crawling along to 3. Soft walls of cups or movement in 3 dimensions is shifting substrate around and disrupting the earliest phases of root development. Transparent walls okay for monitoring progress but need opaque trays to eliminate light during incubation & vertical lifting only until you are at stage 3. I think YOU need to think of 2-6 oz sized cups to go from stage 1 to 2. Focus on technique and dispel perceived notions on what really are critical factors impacting results? I can explain if not clear. For example: substrate obviously not flying out of your vessel. However, the physics involved is forcing shifts and pressures that would be equivalent of earthquake on “earth scale”. The hairy part of roots are the billion square footage multiplier that gives a plant robust access to its environment. I am not positive this was harmful to your specific plants; just saying like at the micro-root structure of plants online…even an electron microscope level magnification…that plant architecture stuff DEFINITELY getting messed up while hobbling along through stage 2.
What’s your grow light intensity for TC?
3:52 i laugh at what your are missing: get yourself a black light flashlight and some other wavelength light sources…your leaves will explode with hidden colors you can’t see initially under traditional light sources. You will never have had as much fun in dark with your black light flash light in your entire life….
What soil mix is it in?
I have a thai in stratum rn and it’s in a enclosed humid environment. It’s been doing well but should i water the stratum😭 the stratum is moist
You should only be adding water when needed. If your stratum is still moist there is no need to add more water as you’ll run the risk of root rot. Hope this helps.
I bought tissue culture thinking it's starter plant. I opened it and to my shock, "what the f is this"? Checked my order and It says, "tissue culture", lol!
Oh goodness, I'm gonna kill it, wish me luck! 🤞
👀🫣lolol no you’re not!! Sending you all the positive planty vibes!! Hope the videos are helpful and your TC grow and thrive!
Would you recommend stratum over a perlite moss mix? For taking them out of the baggies into a dome cup
Hopefully it comes out with maturity. 🫶
I moved it closer to the light source to see if it will help!