This was so helpful. My teacher explained electrophoresis but it made no sense. She never explained that the negatively charged DNA goes to the positively charged end or that you were only adding fragments of DNA.
So this So normal? Due to the slight variation of our DNAs when they are cut with the restriction enzymes the length of the fragments will vary from one to an another individual. In addition, this can be used to detect any mutation.
Great lecture! Thanks for posting. So those last fat bands that are actually one close to, or top of, the other are bands that haven't still separated because we decided to end the test till that point, or it is their natural "closeness" to each other on this particular test, and that is why we are seeing them as a large band?
Fat bands can result from either. Sometimes, no matter how long you have the electricity turned on, because the bands are close to the same size, they'll stay close together or even on top of one another. If, however, you begin to run the gel and turn off the power before the DNA spreads out, you'll see fat bands that you wouldn't see if you ran it longer.
RFLP is the process that cuts the DNA using restriction enzymes. It's sometimes called a "digest" because an enzyme is being used to break down a molecule (DNA). Gel electrophoresis is the technique used to see the pieces of DNA after they've been digested.
Good question-probes are not typically used in DNA fingerprinting unless there's some new technique that I'm not aware of. Instead, probes are useful in determining which genes are used in which parts of the body. Probes are radioactively-labeled segments of DNA that are "hybridized," or bonded to open, replicating DNA. For example, If you are attempting to find out if a particular protein is used in the brain, you might tag a gene for the production of that protein with a radioactive isotope. That gene will hydrogen-bond to the DNA in parts of the brain which are using that particular gene. The radioactive label allows scientists to see the area of the brain where the probe is bonded to DNA on a scan. This indicates that those parts of the brain are actively using the gene for that protein.
I just scrolled down to some other comments, and forgot about blotting. If you are asking about probes used in Southern blotting, they are not used in RFLP, but are used to preserve a record of the banding pattern. See the comments below.
Southern blotting is when those RF have been moved to the solid membrane to add the probe and then exposing it to the X ray and when this done now you can see the fragments and their length = )
Thank you finally someone can explain it very good
This was so helpful. My teacher explained electrophoresis but it made no sense. She never explained that the negatively charged DNA goes to the positively charged end or that you were only adding fragments of DNA.
omg I was so confused about this technique, my book made it seem complicated but you just made it so simple :D thanks a lot!!
So this So normal? Due to the slight variation of our DNAs when they are cut with the restriction enzymes the length of the fragments will vary from one to an another individual. In addition, this can be used to detect any mutation.
Excellent explanation! Thank you
Thank you
This was helpful to me, thank you
Thank you ❤!
thank you so much! This video is absolutely helpful :D
Thank you!! the replies also helped...XD
Amazing I understood
this was very helpful...thank you
Thankyou!! it helped me a lot
Great lecture! Thanks for posting. So those last fat bands that are actually one close to, or top of, the other are bands that haven't still separated because we decided to end the test till that point, or it is their natural "closeness" to each other on this particular test, and that is why we are seeing them as a large band?
Fat bands can result from either. Sometimes, no matter how long you have the electricity turned on, because the bands are close to the same size, they'll stay close together or even on top of one another. If, however, you begin to run the gel and turn off the power before the DNA spreads out, you'll see fat bands that you wouldn't see if you ran it longer.
:) Thanks for making it clear.
Very helpful
What is the difference between rflp and gel electrophoresis
RFLP is the process that cuts the DNA using restriction enzymes. It's sometimes called a "digest" because an enzyme is being used to break down a molecule (DNA). Gel electrophoresis is the technique used to see the pieces of DNA after they've been digested.
You use gel electrophoresis to detect the length
how about dna probes? does they have a part in dna fingerprinting?
Good question-probes are not typically used in DNA fingerprinting unless there's some new technique that I'm not aware of. Instead, probes are useful in determining which genes are used in which parts of the body.
Probes are radioactively-labeled segments of DNA that are "hybridized," or bonded to open, replicating DNA. For example, If you are attempting to find out if a particular protein is used in the brain, you might tag a gene for the production of that protein with a radioactive isotope. That gene will hydrogen-bond to the DNA in parts of the brain which are using that particular gene. The radioactive label allows scientists to see the area of the brain where the probe is bonded to DNA on a scan. This indicates that those parts of the brain are actively using the gene for that protein.
I just scrolled down to some other comments, and forgot about blotting. If you are asking about probes used in Southern blotting, they are not used in RFLP, but are used to preserve a record of the banding pattern. See the comments below.
so RFLP is just the DNA after its been cut up by the restriction enzymes?
RFLP is the process of digesting the DNA with the restriction enzymes. The DNA after it has been cut up is referred to as fragments.
And the electrophorese too but with hybridation
not just i thank you
Is RFLP and southern blotting the same?
Southern blotting is when those RF have been moved to the solid membrane to add the probe and then exposing it to the X ray and when this done now you can see the fragments and their length = )
Correct! RFLP separates the bands, blotting techniques, like Southern blotting preserve a record of the banding patterns.
a bit louder please ???
i can barely hear u