*Thankyou so Much Kenneth* I have to ask that in order to develop a marker do we need to know the sequence of the trait? and how we know that the band we got is not any other piece of DNA have same bp we need
A marker must be linked to a specific trait. In some cases the marker may consist of a DNA locus which is upstream or downstream of the specific gene or genes which regulate the trait. You can develop a locus specific primer for the gene or genes which control the trait OR alternatively select a pair of loci which are on either side of the gene. Two loci reduce the likelihood of error which may be cause by genetic recombination.
Thank you so much For the help. We know about genes either by linkage mapping or by sequencing, right? So we plan marker according to that information?
Thank you for your query Ashu: Markers are developed by identifying specific phenotypical traits such as drought tolerance and then correlating these traits specifically to a specific gene or a family of genes which are likely to be linked to the trait. The process of marker development is based on statistical validation of the linkage between a marker (DNA locus) and its associated trait. I hope this brief explanation will answer your question.
Sir your explanation is superb sir kindly start your proper videos on molecular genetics specially SNPs marks and others
Thank you Sir. Appreciate your kind words.
superb explanation
Sir how do protein markers work under stress conditions?
*Thankyou so Much Kenneth* I have to ask that in order to develop a marker do we need to know the sequence of the trait? and how we know that the band we got is not any other piece of DNA have same bp we need
A marker must be linked to a specific trait. In some cases the marker may consist of a DNA locus which is upstream or downstream of the specific gene or genes which regulate the trait. You can develop a locus specific primer for the gene or genes which control the trait OR alternatively select a pair of loci which are on either side of the gene. Two loci reduce the likelihood of error which may be cause by genetic recombination.
Thank you so much
For the help. We know about genes either by linkage mapping or by sequencing, right?
So we plan marker according to that information?
How markers are developed for doing marker assisted selection
Kindly help
Thank you for your query Ashu: Markers are developed by identifying specific phenotypical traits such as drought tolerance and then correlating these traits specifically to a specific gene or a family of genes which are likely to be linked to the trait. The process of marker development is based on statistical validation of the linkage between a marker (DNA locus) and its associated trait. I hope this brief explanation will answer your question.
How Markar assisted selection role of medicinal plants trait?
Kindly help me
@@KENNETHFRANCISRODRIGUES how Markar assisted selection role of medicinal plants trait?
Kindly help me