This is fantastic as usual, thank you! One small mistake, I think. Around 1:37, you mention that the growing DNA strand uses "this deoxy" part of the dNTP to grow. I believe that the "deoxy" part of dNTP refers to the 2' H (in the "oxy" version, aka. RNA, there's a 2' OH). But chain elongation happens because of a nucleophilic attack from a 3' -OH! Which is why, when you make a ddNTP (which has a 3' -H instead of a 3' -OH) you don't get any chain elongation. If any viewers here want to see more, I recommend the first part of this tutorial: binf.snipcademy.com/lessons/dna-sequencing-techniques/sanger-dideoxynucleotidenation.
Awh they must have changed the URL. Thanks for pointing that out! I think it’s here now: binf.snipcademy.com/lessons/dna-sequencing-techniques/sanger-dideoxynucleotide
Please never stop recording these 😂😂 you've helped so much w/ my bioinf class
Thank you! 🇧🇷
This is fantastic as usual, thank you! One small mistake, I think. Around 1:37, you mention that the growing DNA strand uses "this deoxy" part of the dNTP to grow.
I believe that the "deoxy" part of dNTP refers to the 2' H (in the "oxy" version, aka. RNA, there's a 2' OH). But chain elongation happens because of a nucleophilic attack from a 3' -OH! Which is why, when you make a ddNTP (which has a 3' -H instead of a 3' -OH) you don't get any chain elongation.
If any viewers here want to see more, I recommend the first part of this tutorial: binf.snipcademy.com/lessons/dna-sequencing-techniques/sanger-dideoxynucleotidenation.
the link is broken
Awh they must have changed the URL. Thanks for pointing that out! I think it’s here now: binf.snipcademy.com/lessons/dna-sequencing-techniques/sanger-dideoxynucleotide
dNTPs can most certainly be labeled with S35 despite what the notation says. I've sequenced thousands of bases that way.