How To Clone Mushrooms
ฝัง
- เผยแพร่เมื่อ 21 ก.ย. 2018
- Cloning mushrooms is easy! Learn the process used to clone wild mushrooms in the lab, which can eventually be used for commercial cultivation.
In this video, I take a cultivated King Oyster Mushroom, remove a piece of tissue from inside the cap, and clone it on agar.
I'll also show you a few wild clones that I made using this very same process.
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This guy looks straight into your soul
Swampert D. I was literally about to post this 😭😪
@@Ankh_Hippy dude same here!
Or slightly above it
it's the mushrooms
Chip Osgood he’s seeing the souls of millions and it’s working through the lens ...mushrooms are dope
Banana for scale, good old times.
I understood that reverence
Remote control or coke can also works lol
Lol
One of us! One of us!
I always used a AA battery
Thanks for showing what contamination looks like. No one ever shows what to watch out for.
Yeah, but it kind of makes sense that they don't. For every contaminant someone shows you, there's a thousand others they can't. A much better way is to simply learn what healthy mushroom mycelium looks like, then assume everything else is a contaminant.
I'm so happy I found your videos. I'm a science teacher trying to get my students interested in outdoors and gardening. I think they would be fascinated with growing mushrooms. Also I want to learn myself :) I'm going to check out your website. Any suggestions for first time growing? Thank u!
Awesome! Glad you like them :) My suggestion would be to start with a "kit" to get an idea for the process- then it's down the rabbit hole- happy growing...
I wish you were my teacher! Greatings from germany
Invest in doing things right. If u invest in the right substrates, sterilization procedures, flowhoods or glove box, obtaining sterile cultures... It becomes very easy. It's difficult if u cut corners, and mistakes will cost u more down the road then investing in proper procedure.
I wish I would've had you as a science teacher. My current Biology Professor down plays anything on mushrooms, in fact he gets annoyed whenever I bring up fungi or mycology. I kinda feel most of the science community has a superiority complex when it comes down to the mycology department?🤷♂️
Excellent. Anthony you are so good at spreading the word. Thank you and good job.
Thanks for Sharing Tony ... You are a genius. And i Love how you've done your operations so carefully. you are a Champion
About time man you needed to have done this video long ago. I have been wondering how to do this, Thanks!
Your right, I've been slacking!
@@FreshCapMushrooms how much higher is contamination rate if i do it in a plastic box that i cut 2 holes in for handling.
Flow discipline that you were mentioning is something that never occurred to me. That's really smart keeping where you're going with your transfer/clone etc as close as you can to the hood.
I love the content you put out. Keep doi g the great job!
Thanks so much!!
Great vid!! Thank you for being so thorough
Love your vids! You're the man
Thanks heaps. Lots of helpful information and great examples.
Thanks for sharing your knowledge dude appreciate it
Nice video brother, i really appreciate it!!
The precision makes me so happy
You’ve got a great channel, already made grains thanks to your vid, soon will graduate to agar.
Great video...would love to see a video explaining cleaning up a culture also. Oh! I just got the tshirt I ordered in, and duuuudde...I love it! Super quality
I just saw the transfer video, lol. I must have missed it before.
Couple questions:
Is this tryptic soy agar? Incubate at room temp? How long are the plates good for when stored in fridge or freezer?
Love your videos man!!
Dude such great vids. This guy is great!
Awesome! I shared this with students from my recent class. Very helpful info. Keep it up =)
Thanks!! I will definitely keep it up 😀
The amount of eye contact in this video is proportionally equal to its awesomeness!
Awesome!! Thanks brother
That oyster looks so yummy ~
Really enjoyed your video. Thanks
Thanks for watching!!
You should make a video on maintaining/storing cultures, spawns, and mushrooms
Hi and thanks for the videos. They are really instructional and easy to understand.Maybe you answered this one already. Where can we store, what temperature and for how long both the empty agar dishes and the ones containing mycelium.
TY Tony. This a big help to me.
beautiful work mate
Excellent work...cheers..
Thanks Tony!
Thanks for sharing, very informative
Thank for sharing. Great video
Best mushroom videos on TH-cam! Every mushroom lover and grower should watch your vids!
Video suggestion; how to make a spore print/spore seringe and how to properly store them.
Can you explain what is the next step in the process?
And did you find any useful data answering your question?
@@abdullahzayadin4671 I'm not sure I understand what your asking? As far as making spore prints my research has taught me, you take the mushroom cut off the stalk/stem with sterile scissors or a scalpel, making sure to cut it flush so the cap can rest on the paper, then put a drop of water on the top of the cap and place a glass over the whole thing overnight. To make spore syringes you take the spore prints and put them in the desired amount of water and allow the spores to leave the paper and become suspended in the water. Finally sucking the water up into the sterile syringe. As for storing them I'm still not sure, all I know is keep them out of light and high heat
For active mushrooms check out arvin fortyseven
jackson.wilmes
Absolutely, this is the best and most useful videos on mushrooms I have ever came across! Thank you and keep the good work!
Great video!
Thanks!
So brilliant video. Likeable guy too. 10/10!!
So straight forward and concise! The best in the field!
Excellent video Sir, thank you very mush sir for you are giving good information...
It's Helpful. Thanks
Thank you so much,
Now I have to find out how to grow mushroom using this culture on petri dish...
Great video! Cloned!
Thanks!
@@FreshCapMushrooms I just watched it for a second time and wanted to point out one thing. Your workspace has a flow Hood. A laminar flow hood is built differently. Just to be super anal and Technical about it. But still super amazing video. Totally filled in some blanks for me in-between the reading and the actual practice of taking tissue samples. Thank you very much for taking the time to make this video!
Your right, technically this is a "flow cabinet" or similar, not a hood. But I have always called it a flow hood, so old habits I guess. Thanks for watching the video and glad you could take something from it!
Oh SNAP! What is that monster mushie! Wow!
SO COOL :D, feel like I'm a scientist!!!
Hi
Hello, thank you for the informative video. I tried the king oyster and it worked, but not for portobellos and enokis. Any suggestions for why that might be the case?
thanks Tony
I love your videos but it would be nice if you making a video processing of the mushrooms grown
Love these vids! Question. How long does an agar culture last/ whats the best way to store it?
Fridge. I am not sure how long can they last tho
Good video man. How long does the culture last in the dish?
1. How do you store the agar plates?
2. How long can you store an healthy culture?
How can you store it and how long does it last?, great video btw
i love the banana for scale
Is it better than doing an spore syringe? Could you make a video about pros and cons of each?
BTW... I started this rabbit hole because of your videos!
Thank you!
You are my hero now! Can you please show us how to prepare liquid cultures?
You need to get mushroom spores and mix them with sterilized water
Thak you so much for making these videos!!
I have some queations;
after you put the mushroom pieces on the agar in Petri dishes, how much days are one going to expect them to be ready, and how is it preferable to store the petri deshis, like in a dark fresh place, with no iight coming in? Then, after we have the spores blooming, can we make them into a liquid cultive?
Love your videos. Just curious to know that how many days does it take to fully colonise.
Thanks! Depends on the species, anywhere from 1 -2 weeks is typical.
if you grow them in deep multch and keep adding to in with they ever stop growing?
youre dope man, thanks
What type of conditions do you leave the agar plates to grow? Like temp, lighting, etc.
Temp is about 21 - 27 degrees C, not sure what it is in Fahrenheit
Thanks for the video. Are you scraping from the flesh or gills? Does it matter if it’s either?
Such great videos!
Could you put a tissue sample straight into liquid media and expect mycelium growth or is that more likely to result in contaminations?
Old comment, but if you're still wondering... It's entirely possible, yes.
Funky beats brother
Information is very good - Music is not good. I've been growing mushroom for a year or so. I'm going to try the cloning process.
I like the banana scale
Great video! Where Do i have to store the Agar plates with the tissue inside?
Hi, I have a few questions about equiptment, and RE USEAGE of materials, the glass agar jars, and the plastic grow bags, are you able to clean them and re use them ? maybe you could do a video about that, letting us know about what we can re use, and what we need to use new again, thanks again, great videos.
I can answer this, most stuff in biology is designed to be disposable, the agar dishes if glass you will wash out media and sterilize them in an autoclave before using again. Most petri dishes like this are plastic and disposable. Most plastic stuff is not designed for reuse even certain metal items like scapel blades are disposable. As a general rule of thumb if you can buy the particular item in bulk (agar dishes, scalpel blades, micrscope slides, syringes, empty grow bags, etc) they are designed to be disposable, I'm sure there are exceptions but I can't think of any. Its a lot more ecomical time and money wise to just buy plastic agar dishes then get glass ones and reclean sterilize and make the growth media each time, off course I recommend having glass ware that is reusable that way if you cannot order supplies you can still work, but when you can go disposable.
Wondering what temperature the agar plates are to be kept at for growth? Interesting stuff
For growing i would say 25-30℃, for storing i would say 2-5℃
i love the music
Omg look at the size of that king oyster 😍
Wonderful video! By any chance, is it possible to clone this ways wild mushrooms such as Boletus Edulis or Lactarius Deliciosus?
Mycospinz
On IG
Great helpful video but sir how I store the plates and how long I use a culture for Micilium growth?
Lots of love from Asia
How long do they last and where to store them once it has developed on the new dish
Could tell me what would be the best way to preserve mycelium or spores long-term? Keeping grain spawn / liquid spores / agar dishes / print? My hypothesis would be a spore print taken in a sterile environment and kept in dry sealed jar.
Hi. I've never tried growing mushrooms before. I don't have access to most of the scientific stuff in my country such as petri dishes. Is it possible to clone a store bought mushroom just by using stuff like jars, pressure cooker and homemade still air box. I just want to start growing on a small scale to enjoy the process at home. I also saw some guy grow store bought mushroom on coffee grains in a Jar, idk what was the process called exactly but something like that would be great. I think it would also be a great idea to make a video about it for kids and people with same problem I have.
Hi, thank you so much for sharing, can I do it with button mushroom ?
Cool video. I do a fair amount of wild mushroom cloning to cultivate indoors
That is awesome, so much fun!
How fresh does the sample need to be?
The mushrooms we get are about 2 weeks old when it reaches our shelves.
Where do you store the Petri dishes for further culture? An incubator ? After the mycelium fully takes over, how to store them for long-term use?
Hi ! I have a question: Which agar you used for cloning mushrooms?
Thanks for the video
How did you get the king oyster long and yellow like that? fantastic.
Could you use high proof ethanol instead of isopropyl alcohol to avoid making the mushroom inedible? Or does isopropyl just work a lot better than ethanol.
How do you prepare the agar mixture? Apart from agar what else do you have to put into it?
Thank you soo much !!
66
Thanks my friend, I want to clone some Shitake or lions mane I find.
after complete colonization of the mycelium on the entire surface of the agar, where and at what temperature and how long can the mycelium be stored?
Excellent. Did you cut the strips of parafilm or buy them that way? I wish I would have thought of that a few years ago. lol
I use to cut plastic wrap rolls to two inch lengths. This was before amazon and didn't work well as I had a lot of problems with contamination. I see amazon does have some parafilm but a science supply might be cheaper.
Hey there! Yes, it comes in a big roll, 1 inch wide, I cut the strips with scissors and have them ready. A big roll lasts a long time
how did you make your set up with air filter HEPA?
Good video, but the music at the end is annoyingly loud.
When you do transfers do you just try to cut the good pieces out?
Also do you have to keep the culture dishes in an incubator or anything?
When doing this do you have to cut from the cap your can you cut from the bottom of stem?
Hi, I have a question.
How to now weather my button mushroom fungus is growing good or not? Because this is my first time to grow fungus.
Hi, How long time you can conserve the Liquid culture and the discs and where ? Thanks
Has anyone uploaded videos of original mushrooms versus their clones to compare?
There is a tiny reishi mushroom growing on a hackberry stump in my yard, but the surface of the stump is completely white in a patch--it looks like a block getting ready to fruit. Being January, it's growing very slowly.. is it enough mushroom to cut off for a tissue culture, or do I need to wait? There's maybe a half inch of red showing on the fruit body.
How many times is it possible to ”reclone” ? Meaning you manage to get the fruiting bodies an repeat the operation indefinitely?
Is it best to take the tissue from the cap or the stem?
I wish i could work with you to grow mushrooms
How about smaller mushrooms like pan cyan? Can you still get a tissue on it for agar and get mycelium spores?
Good
Very helpful. Just a quick one, the ziplock is really necessary considering you already sealed it with the parafilm? Doesn`t it stop the oxygen to get inside? Cheers from brazil.
It is better to put them in a bag just to make sure as little contaminations can get in
Can you reuse the petri dish? Or must you just throw it away?clean it with alcohol and or expose it to uv light?