THANK YOU SO MUCH, I've been looking for a proper materials list and now there's a list with links to their sources to purchase as well?! Amazing, you seriously rule C:
Thank you so much for this!! I’ve been searching for two days trying to find a tutorial on this and learn how to do it. I received a diaphonized specimen for Christmas and was immediately intrigued.
Just finished watching all your videos!! I'm wondering how you store all of these materials? I live in an attic so it gets pretty hot up there. Considering it may take me a while to gather all the materials I want to make sure I don't spoil any of these chemicals.
Mg scales are cheap on Amazon now (7 yrs ago a little less so). A good source for ethanol is Lab Alley. I actually got 200 proof (100%) for cheaper than the 95% - but I was buying 5 gal. They don't sell really small amounts. Everclear is of course 95%, but not all US states carry it (some only do 75.5% - but you can dry it to 95%. Lots of youtube on that topic). I would love to do this diaphonization process
Quick ?.. is it ok to use 10% neutral buffered formalin or is strictly buffered formalin or formaldehyde necessary? I believe I read that neutralized formalin is not as good of a fixative as buffered f... but I'm not sure.
Do you know of any place that sells DIY kits for diaphonized clearing and staining. I’m looking for a place that may sell the chemical and specimen all in one kit. Thank you.
Hey, so kind of an odd question. I have been looking everywhere to properly skin a mouse while still keeping the tail and feet attached, but am having a really hard time finding it. Any tips or know anywhere with a good tutorial?
+Megan Keller There are no odd questions here!! I do not know of any tutorials on how to skin a mouse while keeping the tail, hands, and feet. All I kept finding were how to skin a mouse for taxidermy, where they cut at the hands, feet, and tail, keeping them with the skin. I skinned my specimens very carefully, taking time to trim the skin as I cut away from the hands, feet, and tail. I was thinking about making a tutorial on how I skinned my mice specimens?
+TacoKel okay cool. I just didn't know if there was another way D: if at any point you do make one, that would be amazing!! but if not, I can manage being super careful and tending to the specimines to make sure they are skinned pretty :) lol Thanks a ton! very much appreciated!!
Thanks for the videos! I just have a question that I'm hoping you could answer: What if the mouse has fur? Would I have to remove the fur first, or would it fall off on it's own while I'm preserving it?
For the process of diaphonization, if the specimen had fur, you need to skin it and remove the fur. The skin and fur prevent the chemicals/dyes/stains from penetrating the tissues and staining the bones/cartilage, and from digesting the tissues. (:
Hi, great vids! I have a question: for materials you have 10% buffered formulin, in the fixing notes you said 70 formulin:30water. Thats 70ml of buffered 10% formulin? Just making sure 👍
Hello, I was planning on doing this but I was told the trypsin wasn't actually that useful since it was the KOH that was doing most of the work, but you're saying it is absolutely necessary...what is it then? :/
So are you doing a 70:30 from a 37% formaldehyde to get a 10% formalin, or are you taking a 10% formalin and doing a 70:30 mix of that? Its confusing in the list...
The process was developed in 1977 so it's been around for a really long time. So from the time of this video that was less than 40 years ago. Wow! That really is a long time.
hello! great video - very helpful! i just started getting into diaphonized specimens and have a couple questions - was wondering if you could help me? first is, how do you get your specimens to float in the middle of the glycerine? mine float to the top and don't stay in the middle of the jar. second, is it safe for them to be in light (like a window), or will that damage them? thanks in advance for your info!
Thank you so much!!! :D I really appreciate that!! 1. I've found that if you place the specimen in a jar that is too large for it, the specimen will float to the top and not really "sit" well in the center of its container. I would suggest to try a smaller, slightly snug, holding container! 2. I believe that it is completely fine for them to sit by a window. The natural, white light really highlights the intricate beauty of each specimen! I haven't heard of anyone really having a problem with any specimens by light! Hope this helps! (:
i live in Denmark where you can't just 10% Formalin Buffered , is there any other juices you can use? maybe surgical alcohol? (sorry if its a stupid question)
If I were to buy specimens already preserved in formalin like from a certain state named specimen company would that still work or can I skip the formalin soak
I really want to start dying bones. I first did it in my Forensic class well I didn't my partner did it I watched he wouldn't let me touch it. Will you please tell me what chemicals, how much and where you got them.
+Shawna Gibson As prices of chemicals and other necessary materials needed can change through out different areas and in other parts of the world, I cannot confidently state an accurate price/cost range. You would have to find the materials in your area (from sites, or nearby stores, etc.) and calculate the costs and shipping costs. I can though say that a very loose price/cost range would be a few hundred dollars or so for everything.
Prices change all the time and depending on your location, things may have to be sourced from other sites than the ones listed. I would recommend clicking on the product links in the description to best get a price range for the products (:
2 questions: When you purchased some of the chemicals at a "science" store, did they ask you for a taxidermist license or another type of license? Can you skin and clean the organs of the specimen before preserving it in formalin?
1. When I purchased my chemicals at this science store in my area, no they did not ask me for any type of license 2. Yes, you can, and should, skin the specimen (if there is fur) and clean out the organs, then place the specimen into the formalin!
You're so cute and your voice matches your personality so much. I absolutely love you and your YT! Where have you been all my life?!!? Hahaha I'm newly into all this "weird" stuff and finally found my kind of people ahhaha
Spooky it is expensive to start for scratch. I think the most expensive thing is alcian blue, and you could just skip this step and stain just the bones. Enzymes are also expensive and we use KOH in our lab to clear specimens, it’s not the best but it works.
im just going to say this pet stores will have a surplus of betas and will throw them away if you ask for them they will give them to you you might get a few weird looks
@@TacoKellz it's just a criticism which you can use to improve your channel. and i've read few articles about it and came to youtube to watch the process, not to read a short version in the description
THANK YOU SO MUCH, I've been looking for a proper materials list and now there's a list with links to their sources to purchase as well?!
Amazing, you seriously rule C:
Altarofdoom If you need any help locating just let me know! Glad this was able to help!
Holy shit. Thank you so much. Actual links in the description. You're an absolute life saver!!
I'm excited that I found this. I've been wondering for a while how to do this and now I can actually try it!
Thank you so much for this!! I’ve been searching for two days trying to find a tutorial on this and learn how to do it. I received a diaphonized specimen for Christmas and was immediately intrigued.
I'm extremely happy I found your channel! Thank you so much for what you're doing!
Thanks for the shout out Kelly! We can't wait to see the next in the series :-)
At-Bristol Science Centre Ross was a definite inspiration! Plus, your channel is AMAZING!! It makes me more excited about science!
Thank you for doing this!!! Bless you for shining light on this obscure practice. So incredibly helpful !!!
wow the tutorial I’ve always wanted but didn’t know I needed????
Just finished watching all your videos!! I'm wondering how you store all of these materials? I live in an attic so it gets pretty hot up there. Considering it may take me a while to gather all the materials I want to make sure I don't spoil any of these chemicals.
Thank you so much for this! I've been searching high and low how to do this and what materials are needed and this is so helpful.
This is awesome! Thank you so much for these videos, this whole process is fascinating to me.
This is awesome!! Thank you for making this!!
I love how adorable you are 😊 explaining all this, and the amount of your dedication to the depth of detail you put into this video! 😁
thank you so much. this is the most helpful anyone has been about this process!
Wow! Thank you so much!! That really makes me happy that my videos have been able to help you!!
Whem! Thank you for all details.
Anyway it’s either good info and joyful describes.
Soo excited I found your channel!
+Heather Ornelas Well, thank you so much!!! :D
Came here from Depop! Thanks for the content! :D
Thank you so much!!
Mg scales are cheap on Amazon now (7 yrs ago a little less so). A good source for ethanol is Lab Alley. I actually got 200 proof (100%) for cheaper than the 95% - but I was buying 5 gal. They don't sell really small amounts. Everclear is of course 95%, but not all US states carry it (some only do 75.5% - but you can dry it to 95%. Lots of youtube on that topic). I would love to do this diaphonization process
Thank you for doing this!! 🙏🏻
It seems like most of the glycerine i’m able to find online is vegetable based. would you recommend a certain type?
This video is legendary. Thank you.
Quick ?.. is it ok to use 10% neutral buffered formalin or is strictly buffered formalin or formaldehyde necessary? I believe I read that neutralized formalin is not as good of a fixative as buffered f... but I'm not sure.
Nice videos. For money reasons we use KOH for clearing the specimens, as enzymes are very expensive.
Do you know of any place that sells DIY kits for diaphonized clearing and staining. I’m looking for a place that may sell the chemical and specimen all in one kit. Thank you.
Hey, so kind of an odd question. I have been looking everywhere to properly skin a mouse while still keeping the tail and feet attached, but am having a really hard time finding it. Any tips or know anywhere with a good tutorial?
+Megan Keller There are no odd questions here!! I do not know of any tutorials on how to skin a mouse while keeping the tail, hands, and feet. All I kept finding were how to skin a mouse for taxidermy, where they cut at the hands, feet, and tail, keeping them with the skin.
I skinned my specimens very carefully, taking time to trim the skin as I cut away from the hands, feet, and tail. I was thinking about making a tutorial on how I skinned my mice specimens?
+TacoKel okay cool. I just didn't know if there was another way D: if at any point you do make one, that would be amazing!! but if not, I can manage being super careful and tending to the specimines to make sure they are skinned pretty :) lol Thanks a ton! very much appreciated!!
+Megan Keller Of course! I'm planning on making the "How I Skinned My Mice/Specimens" video this winter!
th-cam.com/video/5eO1DkXtUCM/w-d-xo.html doesn’t give instructions but it can definitely be done! Not sure if you’d even still need this after 6yrs! 😃
Thanks for the videos! I just have a question that I'm hoping you could answer:
What if the mouse has fur? Would I have to remove the fur first, or would it fall off on it's own while I'm preserving it?
For the process of diaphonization, if the specimen had fur, you need to skin it and remove the fur. The skin and fur prevent the chemicals/dyes/stains from penetrating the tissues and staining the bones/cartilage, and from digesting the tissues. (:
Thanks for the reply. This helps a lot :)
Hi, great vids! I have a question: for materials you have 10% buffered formulin, in the fixing notes you said 70 formulin:30water. Thats 70ml of buffered 10% formulin? Just making sure 👍
Hello, I was planning on doing this but I was told the trypsin wasn't actually that useful since it was the KOH that was doing most of the work, but you're saying it is absolutely necessary...what is it then? :/
So are you doing a 70:30 from a 37% formaldehyde to get a 10% formalin, or are you taking a 10% formalin and doing a 70:30 mix of that? Its confusing in the list...
The process was developed in 1977 so it's been around for a really long time. So from the time of this video that was less than 40 years ago. Wow! That really is a long time.
hello! great video - very helpful! i just started getting into diaphonized specimens and have a couple questions - was wondering if you could help me? first is, how do you get your specimens to float in the middle of the glycerine? mine float to the top and don't stay in the middle of the jar. second, is it safe for them to be in light (like a window), or will that damage them? thanks in advance for your info!
Thank you so much!!! :D I really appreciate that!!
1. I've found that if you place the specimen in a jar that is too large for it, the specimen will float to the top and not really "sit" well in the center of its container. I would suggest to try a smaller, slightly snug, holding container!
2. I believe that it is completely fine for them to sit by a window. The natural, white light really highlights the intricate beauty of each specimen! I haven't heard of anyone really having a problem with any specimens by light!
Hope this helps! (:
How do you dispose of your chemicals?
i live in Denmark where you can't just 10% Formalin Buffered , is there any other juices you can use? maybe surgical alcohol? (sorry if its a stupid question)
Mishka Andersen Hrrm, is there any way to obtain any type of formalin solution?
You could try contacting an anatomy lab for the chemical
If I were to buy specimens already preserved in formalin like from a certain state named specimen company would that still work or can I skip the formalin soak
casey starkel if they’re already fixed you can skip this step.
I have a 5' corn snake that I recently had to euthanize. I would love to do this to honor his memory. How much of each product would I have to use?
Hey can you make a video specifically about fixing a specimen in formalin?
This is awesome. Thanks.
Where did you go?? I just found your page and this is so interesting but you don’t post anymore 😭 come back and get titktok famous off of this
Where did you find your Alcian Blue 8GX I can't find it for less than 100 dollars online
Donald Cox it is expensive. You could just skip staining the cartilage as an alternative.
Once the diaphonization is done, could I put the skeleton in a mold and put it in like an epoxy cube?
Issei Hyoudou hey. Did you manage to do that?
QUESTION! Lol can i use 90.5% ethyl or does it absolutely have to be 95%?
I really want to start dying bones. I first did it in my Forensic class well I didn't my partner did it I watched he wouldn't let me touch it. Will you please tell me what chemicals, how much and where you got them.
+Katana Masteller hello! The video has all the chemicals needed to diaphonize, the description also has them listed aswell.
When you go buy snake food to diaphonize it XD
cool vid!
What is the overall cost for all the chemicals?
Overall how much did this whole thing cost?
+Shawna Gibson As prices of chemicals and other necessary materials needed can change through out different areas and in other parts of the world, I cannot confidently state an accurate price/cost range. You would have to find the materials in your area (from sites, or nearby stores, etc.) and calculate the costs and shipping costs. I can though say that a very loose price/cost range would be a few hundred dollars or so for everything.
Im in love
How much did you buy each thing? (gallons ect.)
Prices change all the time and depending on your location, things may have to be sourced from other sites than the ones listed. I would recommend clicking on the product links in the description to best get a price range for the products (:
You can buy them live or frozen... LIVE?!?!?!
what percent of hydrogen peroxide did you use
2 questions:
When you purchased some of the chemicals at a "science" store, did they ask you for a taxidermist license or another type of license?
Can you skin and clean the organs of the specimen before preserving it in formalin?
1. When I purchased my chemicals at this science store in my area, no they did not ask me for any type of license
2. Yes, you can, and should, skin the specimen (if there is fur) and clean out the organs, then place the specimen into the formalin!
nice!!
Don't use jars with metal lids, they don't seal well and leak. Buy standard specimen jars.
Or wax the lid
what do I do with my live specimen? xD D'x
You're so cute and your voice matches your personality so much. I absolutely love you and your YT!
Where have you been all my life?!!? Hahaha I'm newly into all this "weird" stuff and finally found my kind of people ahhaha
How much does all of this roughly cost?
Spooky it is expensive to start for scratch. I think the most expensive thing is alcian blue, and you could just skip this step and stain just the bones. Enzymes are also expensive and we use KOH in our lab to clear specimens, it’s not the best but it works.
Nice
Step 1: take out a second mortgage on your home.
Step 2: obtain a master’s degree in chemistry
Step 3: accidentally break the fragile specimen
im just going to say this pet stores will have a surplus of betas and will throw them away if you ask for them they will give them to you you might get a few weird looks
Are animals killed FOR this procedure or is this done with already dead creatures?
Idol Princess Rinny I use specimens that were dead when I found them or were already dead when I came into possession of them.
And what about those live specimens you mentioned, do you kill them yourself?
Crumpit Pennant I don’t use live specimens.
@@TacoKellz what about people who do?
Crumpit Pennant I cannot control the actions of others, I can only control the actions of myself.
the fact that shes hot and shes teaching me shit, i like that
If you buy live specimens. you're a psycho.
Thankyou! I saw that comment too 😟
So pretty
i love you
Live or frozen 💀 that’s cold
Dam thats expensive
When I searched for this tutorial, the last thing I thought I was gonna see was a hot chick showing me how to do this LOL. I am happy now.
You are beautiful!!
I'm in lust!
I love this stuff but god damn dont recommend buying them live for this.
Why on earth would you buy something living when there are so many dead to choose from 🤦♀️
You are cute as hell
Sad all the helpless animals that are murdered so people can make art projects...
Her specimens are for animals to eat... they were killed for food not for "art" wtf. You could always just get roadkill aswell
it's very informative, thank you! but the way you goof around and smile everytime anoyes me. it makes you wanna say - common! get to the point
Alexi Novoselov if you would like a more “get to the point”approach,” please read the description instead. (:
@@TacoKellz it's just a criticism which you can use to improve your channel. and i've read few articles about it and came to youtube to watch the process, not to read a short version in the description
“You can buy your specimens live or…”
No. Just no. Never ever do that. We are honoring the dead here, not murdering the living for pretty trinkets.