GCaMP | GCaMP calcium indicator | How GCaMP works? | GCaMP application in neuroscience
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- เผยแพร่เมื่อ 19 ต.ค. 2024
- This video will talk about a genetically encoded calcium sensor GCaMP. GCaMP is a genetically encoded calcium indicator (GECI) initially developed in 2001 by Junichi Nakai. It is a synthetic fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light-chain kinase.
Further reading :
1) A quick review on the utility of GCaMP : www.nature.com...
2) GCamp resource page (includes description of all varieties of GCaMP) : www.janelia.or...
I just started my PhD in neuroscience and I will be using all of this in my experiments for brain mapping, this video and the video on two-photon calcium imaging were the most simplified videos that I will be using in most of my presentations in the future! Previously I have never used a two-photon microscope so both of your videos helped me to clarify all the doubts I had. Thank you!
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Me too
I literally just had to learn about this as I will be doing research in a lab that involves GCaMP, and somehow I had the incredible luck of you posting this amazingly clear and insightful video! Thank you so much Arpan, you have helped so much (with this and all of your other videos)!
Really glad to hear that my contents helped you. Please share my channel link with your friends
Thank you so much! this is incredibly helpful and clear. I'm a physicist doing an intership in neuro and i've been struggling with all of these new concepts in papers, it's great that there are videos like this
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8&
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Thanks cami. Feel free to connect via email. My email is arpanparichha1994@gmail.com. I will be happy to help.
Thank you. This is a really awesome presentation or instruction on Calcium imaging in detail.
You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me
thx a lot, I'm doing a research for this now.
Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance
Fantastic! Thank you !! Have you devled even deeper in GCaMP and Voltage sensors?
Yes
Arpan, you are amazing. How about primary neuronal culture based calcium imaging analysis
In primary cultures people prefer using dyes like Fluo4 , Fura 2. Please share my cahnnel link with your friends and help me to reach big audiance.
Excellent introduction - very clearly explained. Thanks! With your permission I would like to embed a link to your TH-cam video on a webpage that I am creating. Please let me know how I can get in touch with you.
Arpanparichha1994@gmail.com is our business email
can you please make a similar video of electrophysiology? Thank you
th-cam.com/video/CvfXjGVNbmw/w-d-xo.html
Please share my channel link with your friends and help me to reach big audience
You know you are paying attention if you notice the burp at 7:28
There is no burp