Villains Defeats 152 (Spoilers for The Bad Batch)

Death/The Wolf and "Big" Jack Horner from Puss in Boots: The Last Wish
The Plaintiff and His Lawyers from Weird Al Yankovic - I'll Sue Ya
Steven Jacobs from Rise of The Planet of The Apes
Vice Admiral Edmon Rampart from Star Wars: The Bad Batch
Kyodai Ken from Batman: The Animated Series
Dr. Julius No from Dr. No

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In 1st season was Crosshair/CT-9904 , Vice Admiral Edmon Rampart and Lama Su.
In 2nd season Vice Admiral Edmon Rampart and Dr. Royce Hemlock. In 3rd season only Hemlock

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The Bond villain that started it all.

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1:33 Vice Admiral Rampart

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Death Wolf Big Jack Horner

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Villain

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Villains defeats(spoiler/the emperor and kronk's new groove)
Jack horner
George
Emperor kuzco
Yzma
Kronk
Papi
Dr julius