Bacterial Identification Tests: Motility Test (Hanging drop method)
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- เผยแพร่เมื่อ 28 ก.พ. 2019
- Motility is the ability of an organism to move by itself by means of propeller-like flagella unique to bacteria.
If fresh culture of bacteria is available, microscopy is the most accurate way to determine bacterial motility and ‘Hanging drop method’ is a commonly used microscopic technique.
Hanging drop preparation is a special type of wet mount (in which a drop of medium containing the organisms is placed on a microscope slide) to observe the motility of bacteria.
This Video demonstrate Procedure, Observation and Use of Hanging drop method for bacterial Motility test.
This video is very helpful i will recommend it to my friends . The motility test was my weakness but not anymore.
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Awesome video sir
Thanks for this wonderful teaching
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Very clear explanation sir. Thank you so much
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Thanks
Excellent.. Thank you🙏💕
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God bless you💙
excellent. thank you Sir.
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Incredible
Thank you so much
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Excellent explanation
Now my concept is clear and I can give viva without any fear
Thank you sir 😊
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Best of luck for your Viva👍🏼
Very informative video
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Thank u so much sir the video was really helpful❤
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Nice explanation...
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Hi direct second year students
Nice Sir
nice video sir, its really helpful
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Wow ❤ Sir this was so interesting and helpful🎉thank you so much
Glad you liked it!
Very helpful. In college we use the play dough instead of cavity slide. I did this experiment 4 times and I couldn’t observe any motility. So my teacher couldn’t give me the mark. I think that’s because we fix the lens on 100x power , I will try again with 40x power. Thanx
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Wgat if there isnt any cavity , will we be able to observe bacteria mobility.
Thank you so much sir ❤️
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Ham to fan ho gaye apke 🎉
I'm glad that this was helpful for you.🙏
Plz tell the principle of hanging drop method.
“I like your video!”
Thanks
Nice video
Thanks
If you open the cover slip with sample of bacteria in air for a long time that u do now then bacteria could be change their character and started to reaction with environmental organisms...how can do u this process... sir??
Very nice 👌👌
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Its helpful
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Sir you took another cover slip but didn't applied on slide na 😕
Sir Do we need to use the fresh broth for viewing motility assay ...or can we use the refrigerated broth also
Freshly prepared one.
Thank u Sir. Great explanation. One question, can plasticine be used in place of petroleum jelly?
I'm glad that this was helpful for you🙏🏼
Coming to your question... Yeah you can use Plasticine provided it should hold cavity slide and cover slip properly.
@@the_bioway Alright Sir, thanks😄
Hi malhari how are u
I love microbiology
Hlo is it possible to view the border in 40x too or in just 10x.. My teacher keeps asking for that in observation.. I'm not really able to get it.
At 40x or 10x you can see only the border but to see bacteria you need to go for higher magnification power
Sir.. You have to off the bunsen burner flame
But sir why the video procedure are not displayed?
Why did u use petroleum jelly only to coverslip?
To make sure only corners of coverslip getting attached
does this method need heat fixation?
No
Sir we can see any type of bacterial movement through this procedure?
Yes
Sir , why we focuse the edge of the drop in this method ? 🤔🤔
There is higher probability of finding motile bacteria due to oxygen concentration at edge
@@the_bioway Thanks a lot sir 😊
❤
💕💕💕
Sir please can Answer me the heating of loop donot kill bacteria ?
Heating the loop kills unwanted bacteria... So it becomes sterile (free from bacteria) and after cooling it...then it can be used to transfer bacteria of our choice....
Thank you sir god bless you
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No.. We have ro sterilize the loop.. We are doing many cultures and and we have to transfer many of the culture to cover slip.. So we have to sterilize it
Heating of loop kya huta hai mam
But we are not dropping any thing than why its called as hanging drop method
Because bacterial sample we have taken on coverslip is now in inverted position against the cavity side.... when we we observe it under microscope ... Which results into drop hanging from coverslip
👍👍👍
Thanks
How to do this experiment with solid media
You can refer following article for Motility Test using Semi-solid Media
microbeonline.com/tests-bacterial-motility-procedure-results/
@@the_bioway Tomorrow is our microbiology lab exam. But I'm still confused about making hanging drop from a solid media.
Hanging drop method doesn't use Solid Media.
I have a question
Why do bacteria move in edged
Bacteria move towards edge due to high oxygen concentration at edge
Tq so much for your certain help.
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I am worry for the subject from whom this sample is taken😩😩😩😭
Hiii
Malhari how are u
How to report?
Reporting is done as Motile, non-Motile and sluggishly Motile organisms
can I hear a cat in the background. I hope not.
Not at all... 😅
cats are great
ايزي😂😂😂😂😂😂😂😂😂😂😂😂😂😂😂😂
Thanks
Nice video
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Nice sir
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❤️