Sterilizing Liquids: Autoclaving & Filtration

The autoclave and sterile filtration are two common techniques we use to sterilize liquids and protect patients. In this demo, we’ll make some culture media and split it into three fractions. Then we’ll compare an untreated sample (control) with one from the autoclave and a third that’s been through a sterile filtration process.
The BioWork certificate program will teach you the foundational skills you need to begin a career as a process technician for a biotechnology, pharmaceutical, or chemical manufacturing company. BioWork is offered at NC Community Colleges around the state. To find the one nearest you, visit ncbionetwork.org/biowork.
© 2021 NC Community Colleges

[@Valentinfj]

That's awesome! Subscribing to your TH-cam channel was a very wise decision.

[@deyvismejia7529]

Nice video!

[@nekomusic2293]

Super great. Could you show a setup without the bottle top filters? What do we need to sterilize trough a pvdf membrane? I need that information for my mushroom hobby.

[@RemarhBsoul]

I would recommend bottle top filter with pvdf 0.2 um membrane. Ex. From Corning

[@syedrubyadhossen9098]

Great

[@sabanader979]

🔥❤️❤️❤️

[@nivedithasathiamoorthy8767]

Could you please tell what type of membrane will be required to filter - sterilize urea solution ( 0.1% w/v) , lactic acid (88 to 92% concentration), hydrogen peroxide solution (30%w/v)and NaF solution (60%w/v)?

[@brendagrubb1003]

0.2 μm filter poor size is needed for sterilization

[@sridhineshrc5682]

❤🙏