Applying a Simple Stain to a Bacterial Culture

Applying a simple stain to a bacterial culture is a technique that is used for examining the size, shape, and arrangement of a specimen. It uses a dye to stain the cells, making them easier to see under a microscope.
In this experiment, we will demonstrate the steps for applying a simple stain to the bacterial organism, staphylococcus.
Our hope is that after viewing this video, you will feel more confident and be better prepared to try applying a simple stain in a hands-on lab experience.
Free Interactive eLearning Tools: ncbionetwork.org/iet
This video was filmed on location at Gaston College.

[@chulett76]

This is an excellent video that includes all the steps to performing a simple stain including pictures of the speicmen at each magnification including oil immersion. Like the video said..."Now I am ready to go into the lab and perform a simple stain." Thanks!

[@adeyanjugbenga5581]

This is one of the best video I have ever watched on simple staining

[@jamaicansistarobinson7587]

This is succinct and worth its weight in pure gold!

[@codyellsworth7927]

Excellent video. Very clear steps.

[@Fiza16]

Thanks a tonn.. ♥️ searching for this explanation all over TH-cam... Stay happy sir 🙏

[@niveoserenity5862]

Thanks for the upload! Very helpful for my high school science project ^^

[@Abdifatax-sy4kp]

This was a best video of grain stain thanks u alot

[@engelr899]

Excellent explanation

[@osevweayodeji]

Excellent job.

[@JacksonReynolds]

Superb video!

[@rosiedianne1599]

This video is AMAZING

[@chinmayeechavan5724]

Thank you so much!

[@sathyabtech6870]

After long time I do practical examination it was nice experience good memories with my frds and my hole batch member I love microbiology and learn alott

[@akashpawar9542]

Thanks for sharing

[@45bounty]

perfect..thks so much

[@zahraa.t2495]

Thanks 💕

[@user-he9xi8un4b]

Perfect

[@osevweayodeji]

Excellent

[@santhiprinter1306]

Thanks

[@ravikanaujiya6372]

Thank u ❤️

[@GayanRathnasuriya]

Thank you very much for uploading.. Thumbs up!!!!!

[@akinyemikindnessoluwole3352]

Good Day sir thanks for your Delivery
Please why do you Draw the Cycle downside ?
please why do you stain with the Safranin?

[@WastedElephant]

Are you kidding me? You want me to sketch stuff? It's gonna look like a 2 year old did it. I'll just snap a photo lol. 😂

[@abdulmashruf6931]

Experiment 😍👍👉🙌

[@jasongonzales7699]

Do you not need a cover slip before using the oil?

[@leoDvincci]

Nope...

[@shifnajamal7874]

it's very useful video. thanks for this. but i have a doubt, the nose piece of the microscope is touched the oil immersion. is that a correct method?

[@L._Titus]

Yes, it is. The oil is used to keep light from escaping out the sides as it passes between the slide and the 100x objective lens.

[@kooksyt]

@@L._Titus what oil is it?

[@L._Titus]

@@kooksyt It's sold as immersion oil. They used to use actual cedar tree oil, but now most labs use synthetic oils.

[@Cyclonut96]

When dispensing oil, the technician should have not made contact with the oil bottle and the slide, as this might transfer the specimen into the oil bottle and contaminate future observations. Instead, he should have let the oil drop fall down onto the slide, from a higher distance. And I have not even operated my recently bought microscope, LoL.

[@santhiprinter1306]

Send more

[@abudaudrahmani5880]

Sir for the bacterial staining we use 4 reagents 1 crystals violet 2 iodine 3 decolourizer 4 safranine 0.5% but you used only safranine

[@lqr824]

This is not a Gram-stain method, just a single-stain method. Gram-stain is a four-step process and gives you extra information about the bacteria you're looking at that a single-stain method doesn't.

[@gutsandglory7507]

Seems the lens touched the oil at the last stage of the demonstration, Its okay and no problem?

[@patrickdunavan9113]

Yes...but ONLY use the microscope oil with the 100X oil immersion objective lens.Never use the lesser powered objective lenses with oil. With the 100X high power lens, light is refracted through the lens and can be dispersed into different wavelengths, and will come to focus at different points. You need to have all visible wavelengths coming to focus at the same point. The oil minimizes the light wavelength "scattering" so it all comes nicely into focus, and the image being viewed is much better resolved. In brief, the oil gives a much sharper viewing image.

[@WastedElephant]

​@patrickdunavan9113 for any other complete noobs like myself, do not confuse objective magnification with total magnification... sorry to everyone who has taken biology and knows this. Some of us don't know this lol 😅

[@user-ko5dq5qx9w]

I will be the best microbiologist

[@dayangsittishelnasangkula-3731]

i did not see that the slide have been stained or flood with primary stain which is crystal violet...U skip all the way to counter stain.

[@lqr824]

This is not a Gram-stain method, just a single-stain method. Gram-stain is a four-step process and gives you extra information about the bacteria you're looking at that a single-stain method doesn't.

[@hadjadiallo3566]

Why did he draw the circle?

[@amnaalnaqbi5593]

fixing the smear wouldn’t kill the Bactria???

[@chibuikenwanyanwa8945]

I thought my body would never be the same again, but you fixed it. My heartiest gratitude to you Dr Igudia for helping me cure my Hepatitis B Virus successfully.

[@NCBioNetwork]

We are glad you have been healed 🙂

[@natzedwards3551]

3:30 no touchy nose piece

[@troyjesse7833]

What a silly thing to write. If no touchy rotating nosepiece, no focusy on oil immersion.

[@thirdlegada46]

stain stain go away period charot

[@ToanNguyen-cn7wy]

haha

[@mariaafroz2914]

It's pink, not red

[@idkman-rr3bm]

Why am I watching this

[@hasanalsaffar4649]

Perfect

[@shreyanchal4884]

Thanks

[@allaudin5849]

Thanks

[@laminmorison632]

Thanks