The cold alcohol helps the DNA precipitate better than room temperature alcohol (helps with the insolubility). Try chilling it at least 30 minutes before the lab, or longer if you have the time. I have done this without ice-cold alcohol, however, and it still works!
Good day! What factor affecting enzyme that is present or tested here?
Thanks mam demoer.i just to ask why do we need to chill the alcohol and for how long
The cold alcohol helps the DNA precipitate better than room temperature alcohol (helps with the insolubility). Try chilling it at least 30 minutes before the lab, or longer if you have the time.
I have done this without ice-cold alcohol, however, and it still works!