Sir, I am getting "infinity" values or NAN only , not the quantification value even after adding the background as you have suggested in the video. . Any solution? Kindly help me out on this . Thank you so much in advance. It will be a great help.
If you are getting infinity values then you have peaking somewhere in your image where you are selecting. If you are able to acquire the image agin with lower exposure this should fix it. If a single pixel is saturated (I.e. maxed out) then you will get this errro
@@DoryVideo Thank you sir for replying. I wont be able to acquire the image at this moment. I will try to reduce the brightness and then ll try once again for the same image.
Nice Video and I have few concerns about analysis. Since 4 years I am using LI-COR Odyssey. When I was in Radiation Oncology, Emory University I could able to invert 700 and 800 channel TIF image in Adobe Photoshop but I could not able to INVERT 700 and 800 channel TIF file in Adobe photoshop at TTUHSC. Suggestions please.
ramesh K I can't speak as how to invert images in Photoshop, but within Image Studio, you can control the colors by using the various control buttons above the histograms: When you export the images from Image Studio, they will be the same as they appear on your screen.
JAPJAP Altering the appearance of the image on your screen will have no affect whatsoever on the quantification. The analysis is always done off of the original image. And the original image is never altered by Image Studio. Only the screen appearance is changed.
starts talking about densitometry at 4:46
Thanks for the video... How does this compare with image J ?
If you want to measure density of bands then it’s excellent but ImageJ offers other functionality that this is not designed for
Thank very much. It's good for me.
I keep on getting "infinity" values for even light bands, which is very frustrating. Do you have any idea how to resolve this issue?
Did you resolve the problem anyhow?
How do you analyse RGB signals? Do you add them up?
Sir, I am getting "infinity" values or NAN only , not the quantification value even after adding the background as you have suggested in the video. . Any solution? Kindly help me out on this . Thank you so much in advance. It will be a great help.
If you are getting infinity values then you have peaking somewhere in your image where you are selecting. If you are able to acquire the image agin with lower exposure this should fix it. If a single pixel is saturated (I.e. maxed out) then you will get this errro
@@DoryVideo Thank you sir for replying. I wont be able to acquire the image at this moment. I will try to reduce the brightness and then ll try once again for the same image.
easy to listen your English for non native speakers and to understand the contents
Glad it helped!
Nice Video and I have few concerns about analysis. Since 4 years I am using LI-COR Odyssey. When I was in Radiation Oncology, Emory University I could able to invert 700 and 800 channel TIF image in Adobe Photoshop but I could not able to INVERT 700 and 800 channel TIF file in Adobe photoshop at TTUHSC. Suggestions please.
ramesh K I can't speak as how to invert images in Photoshop, but within Image Studio, you can control the colors by using the various control buttons above the histograms:
When you export the images from Image Studio, they will be the same as they appear on your screen.
Nicely done!
This is good news for densitometry! However, I have a concern. What checks are put into the software to prevent or minimize dubious editing?
JAPJAP Altering the appearance of the image on your screen will have no affect whatsoever on the quantification. The analysis is always done off of the original image. And the original image is never altered by Image Studio. Only the screen appearance is changed.