Gram positive and gram negative bacteria (Gram Staining procedure explained)

Hey Friends,
with gram staining, we can distinguish between gram-positive and gram-negative bacteria. The gram staining assay is based on specific chemicals (Crystal violet, Gram´s Iodine solution, alcohol/acetone and safranine/fuchsine).
References:
serc.carleton.edu/microbelife...
microbiologyinfo.com/gram-sta...
Cheers
Henrik
Time Codes:
0:00 Introduction
0:20 Difference between gram-(+) and gram-(-) bacteria
1:05 Gram Staining
2:26 Antibiotics

[@mariecurie4683]

Henrik, your content always makes my day. Your videos are always so professional, interesting and informative. The amazing graphics make science so entertaining... Thank you very much, keep them coming 🔬

[@RookieGamerz-3110]

This video is a blessing. The animation and diagram are so perfect that its hard to forget the concept.

[@adagross2488]

Great easy breasy beautiful microbiology!!!!!

[@aichabee3014]

This video was a really great illustration of the concept!

[@AjitKumar-du6zf]

Thanks! For your well animated explanation thanks a lot always sir!!!!🙏🏻

[@ghousiatabassum3621]

Brilliant explanation ✨🌸

[@peacely191]

Awesome video, thank you 🙏

[@Auraoll]

Amazing video thank u so much you explained in to simple yet so effective ‼️‼️

[@user-tj2pk4nq3s]

Thank you for your efforts to peoples to understand it

[@Burhadedurvesh]

fabulous

[@btsupdateschannel6865]

Thank you so much I understood 🙏

[@Dana-gv7og]

THANK YOU !

[@soumyadipsamanta6391]

Thanks for this detailed information........ I've seen many videos on TH-cam related to this topic........but it's nearly impossible to find someone, giving the proper mechanism like you did.........majority described that gram positive bacteria cannot take the counterstain, they don't even explain the reason for the decolourisation.
But you cleared a lots of confusion.........so thanks again 😊

[@nicnac9147]

Ikr, English aqa explains this so shit

[@HUMAIRAKHAN_1999]

Nice explanation ❤

[@preciousogie9852]

Your videos are great!

[@henrikslab]

Thanks!

[@KhushiVerma-de7it]

Ooh my dear thaknu thku so much ❤❤❤

[@dina3630]

Thank you❤

[@sabakanwal9092]

By treatment with alcohol the outer membrane of gram negative removed and the crystal violet+iodine bond washed out. As gram positive have thick peptidoglycan layer so this bond of (crystal violet+iodine) is not washed out.. So retained.
after that when these both are treated with safranin ( counterstain have almost pink color) the gram negative adopt pink colour and gram positive remain purple due to thick peptidoglycan layer.
It is differential staining procedure

[@nicnac9147]

This helped so fucking muchhh, finally something my textbook wouldn’t explain with the staining

[@user-rk2fm3bn3u]

Thank you ❤

[@melodiousmag]

Thank you sm!

[@enjoyyoureverymoment4381]

Thank you

[@ZingClasses]

Thanks ssir

[@talhachanna9126]

Thanks

[@ruggerogabbrielli6831]

What does crystal violet bind to exactly when used for the first time?

[@radhickasharma7556]

Thankyou ❤️

[@arabdrops7009]

Amazing video!

[@user-kj7zk8lj5k]

STL1 are here !

[@SomeOne-xr7xu]

I don’t get why the outer membrane of gram negative is removed ? And where is the role of lipid in all of this?

[@gavinwightman4038]

The outer membrane is not removed; the crystal violet/iodine is washed out with alcohol in gram negatives due to their much thinner peptidoglycan wall. Gram + retain the violet color.

[@gavinwightman4038]

The Lipopolysaccharide (containing lipid A endotoxin against immune systems) is on the outer leaflet of the gram negative outer membrane.

[@ahmaadkhaan1694]

Crystal Violet is washed out in Gram positive bacteria not in Gram Negative bacteria ..?​@@gavinwightman4038

[@Talpham]

Nice video, love your channel, if you want to do a collab one day hit me up!

[@henrikslab]

Watched some videos on your channel and have to say they are great! Why not? If you have an idea about a collab that makes sense tell me.

[@Talpham]

@@henrikslab Awesome, I'm going on holidays for 2 weeks now, but after that I'm going to work on it, thanks for the opportunity, this will be great!!

[@user-rk2fm3bn3u]

Thank you ❤

[@slaymarie_rdh_ntraining]

Thanks