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Great vedio for cell culture indeed😊

this is so useful thank you so much for making this!!!!!!!

I guess the falcon tubes can be reused if autoclaved no need to incinerate them, I am saying this with respect to universities protocol no idea why its done in professional labs....

Does this really follow aseptic practice? I doubt that。

can you plz tell the concentration of sodium alginate used and also mention the solvent for alginate solution?

Hi Neil, I am facing contamination continuously in my cultured cell. I checked my media and My insect cell media which i prepared manually using 2x sfc media, gentamycin, P/S ,serum and cell culture graded water. Could you please tell me how can I know that which of any cell culture reagent having contamination or not?? I sprayed alcohol vigorously on the reagent bottles, holders, pipettor, washed my hand, wearing gloves, rubbing my gloves with alcohol, keeping my empty plates and plastic container under the uv inside the hood, still contaminated media... :(

Why he put 1.2 instead of 120 ? Someone explain please

Thank you!

Thank u so much 🙏😊🙏🙏

extremely helpful

Very good presentation

Wait why would you wash the cell to remove the medium with the PBS? I mean medium is required for cell growth, why remove it?

wonderful and well presented. hope i could an opportunity to work under your supervision and guidance. i am final year student of biotechnology.

Very nicely explained sir thank you very much 👍

Fantastic, thanks so much!

Is it "safe" to pour cells out of the flask? Especially when working with primary cell lines, would it be better to pipette and avoid the flask's lip? or does it not matter?

very informative and fast. very good technique! Could the counting be made without trypan ? It seems yes ,

perfect explanation! simple to understand and the throughoughly produced visuals really helped

Is breathing on it adding potential contaminating?

Absolutely great

Very helpful 👍

Thank you for sharing your knowledge! Great video

Very thorough love this

Thank you this was very helpful!

Very informative 👍🏻👍🏻👍🏻

thanks so much

No trypandblue?😮

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Very informative; step by step explanation. This is the perfect video I craved for. Nice work, Cross

Thank you soo much!!!! That was brilliant

Very good video , thanks

Excellent and very thorough. A re-upload with louder audio would be fantastic.

An informative video

Mr. Cross, thank you so much for this awsome video. It is really a review and informative for m.. Great presentation and easy to undersand. Kudos from Morocco.

It was very helpful!! Thanks!

Thank you for this nice video

Very nicely explained sir. Thanks a lot..

"Avoid pouring media and reagents directly from bottles or flasks." This is major violation of the sterile technique. Pouring media or PBS introduces aerosolization or spillage of liquids leading to cross contamination or contamination in general. Even though it may seem wasteful, serological pipettes are much cheaper than a ruined culture and loss of cell line.

I love your videos! thank you!

This is an amazing work! Thank you so much!!

Great video, do you have a protocol for MDA-MB-231 cells?

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"HDAC inhibitors enhanced the acetylation of the 20S proteasome, which correlated with an increase in the T-L activity of the proteasome in mouse and human myocardium "

Hi thanks great video , so we was taught to use aseptic technique by spraying anything going in the the fume hood ,

Thank you for this video, however I can't find the tutorial for counting cells :(

How are the cells adhering to the flask enough so they're not dumped out with the fetal calf serum?

Can I ask if there are any cells in the dmem media that you throw out or all cells only adhere to the flask?

Thank you very much. This is very explicit.