Urine Analysis for Abnormal Constituent | Sugar Test by Benedict's Reagent in Urine | यूरिन सुगर
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Basic Cause and reason for Diabetes- • Diabetes Mellitus | Ca...
Diabetes mellitus is a disorder in which blood sugar (glucose) levels are abnormally high because the body does not produce enough insulin to meet its needs.
Type 1 Diabetes Mellitus
In type 1 diabetes (formerly called insulin-dependent diabetes or juvenile-onset diabetes), the body's immune system attacks the insulin-producing cells of the pancreas, and more than 90% of them are permanently destroyed. The pancreas, therefore, produces little or no insulin. Only about 5 to 10% of all people with diabetes have type 1 disease. Most people who have type 1 diabetes develop the disease before age 30, although it can develop later in life.
Type 2 Diabetes Mellitus
In type 2 diabetes (formerly called non- insulin-dependent diabetes or adult-onset diabetes), the pancreas often continues to produce insulin, sometimes even at higher-than-normal levels, especially early in the disease. However, the body develops resistance to the effects of insulin, so there is not enough insulin to meet the body’s needs. As type 2 diabetes progresses, the insulin-producing ability of the pancreas decreases.
Complications of diabetes
(1) Brain, causing a stroke
(2) Eyes (diabetic retinopathy), causing blindness
(3) Heart, causing a heart attack
(4) Kidneys (diabetic nephropathy), causing chronic kidney disease
(5) Nerves (diabetic neuropathy), causing a decreased sensation in feet
Benedict’s solution is a deep-blue alkaline chemical reagent used to test for the presence of the aldehyde functional group -CHO which consists of copper sulfate pentahydrate (CuSO4. 5H2O), sodium carbonate (Na2CO3), sodium citrate (Na3C6H5O7) and distilled water. Sodium carbonate renders alkaline conditions which are required for the redox reaction, while sodium citrate is a complexing agent which complexes with the copper (II) ions to avoid degradation into copper (I) ions during storage.
Principle of Benedicts test
Benedict’s test is performed by heating the reducing sugar solution with Benedict‘s reagent. The presence of the alkaline sodium carbonate converts the sugar into a strong reducing agent called enediols. During the reaction, enediols decrease the cupric particles (Cu2+) present in Benedict’s reagent to cuprous particles (Cu+) which appear as red copper oxide (Cu2O) which is insoluble in water. When Benedict’s reagent solution and reducing sugars are heated together, the solution changes its color to orange-red/ brick red precipitate. The red-colored cuprous oxide is insoluble in water and hence, separate out from the solution. When the concentration of the reducing sugar is high in the solution, then the color becomes more intense (reddish) and the volume of the precipitate increases in the test tube making it clearly visible.
The procedure of Benedict’s Test
1. Pipette out 2 ml (10 drops) of Benedict’s reagent and placed it in the clean test tube
2. Approximately 1 ml of sample (urine) is added to Benedict’s reagent.
3. The test tube is placed over the boiling water bath for 3-5 minutes (can be heated directly over flame).
4. Observe for color change in the solution of test tubes or precipitate formation.
Result Interpretation of Benedict’s Test
1. If the color upon boiling is changed into green, then there would be 0.1 to 0.5 percent sugar in solution.
2. If it changes color to yellow, then 0.5 to 1 percent sugar is present.
3. If it changes to orange, then it means that 1 to 1.5 percent sugar is present.
4. If color changes to red,then 1.5 to 2.0 percent sugar is present.
5. And if color changes to brick red,it means that more than 2 percent sugar is present in solution.
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Watching this video before 1 hour of exam😅
@@jabasaharia5813 😂😂
😂😂😂
Watching just before my viva
No one can explain as good as you thank you so much mam 🙏🙏😌
Thank you
✅Correctly
Koun koun exam se pehle meri trh pdhne aya h🤗😋
Mai kal exam hai
Mera kal
I have exam tomorrow 😂
And I am making my first time and last time preparation through these vedio😂😂
Me
Thank you MAM.... it's totally Amazing... Tomorrow is my practical exam i was thinking i can't.... But seriously u make it very very easy.... Tnku so much 💗💗.... Love from Kashmir 💗
Hi
Watching just before one day of my exam thanku mam
I can't tell you how happy I am.
You are explaining so good. ☺
Easily understood 😊 thank you ❤
Superb same here 🤗
Thank you so much you explained it so well
Mam aap bohot achchese practical samjhate ho 😊😊❤❤ thank you
Amazing video U explain all the terms clearly n Sweetly 🤟✌👍
Thanks a lot 😊
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very nice mam i very easily understood
Thanks for information
Thank you😊
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Thanks for you
Good explained
Very good mam
thank u maam
Thanks you❤
A big thanks from my heart mam ,this is very help full
Super mam thanks
Nice explain
Thanku ji
Video is very Good .But small changes has to be done in this test first we should heat the benetic solution only .why because means whether the benetic solution is expired or not we should check for that .we heat first benetic solution then after few minutes later add urine sample by using droper .
Thank you so much for your suggestion. We will keep thing in mind.
Thank you 🙏
Thank u maam
Thank you 🥺❤️
Thak u
Lots of love from Pakistan ❤️❤️❤️
Thanks mam
Good ma'am
Practical ke 1 ghante pehle dekh ra
Mai bhi
Thank you somach medam.. 🤗
Super thank you so much
Good explanation tq
Which doctor to consult if hemoglobin increases in children aged 15 years۔
Thanks di
5ml Benedict Solution add 0.5ml or 8 -12 drops of urine ok
Right
Thnks mem..🙏🙏🙏
Thank you so much mam
Super 🥰🥰🥰
⭐🌟👍👍👏👏👌👌👌👌👌
Mam alcohol fermentation per video banye.👍👍
Already uploaded
Can we take sucrose instead of glucose
Hello Sir, 2021 se D Pharma ka syllabus change hua kya??
Yes its about to change
Maine kisi Ko protein aati ho ismein to kaise check
Thank you so much mam
Very nice 👌 👍 👏explanations mam
Mam, Biochemistry test practical video or notes
Tqu mam
5ml reagent me 0.5ml urine sample dalte he jiska ratio 1:10 hota h
Why to heat testtube
Jai mata di
Mem kya analysis krke wood ki age ka pata laga sakte h 😳
Plz tell that..During this experiment, after heating, the smell of Urine will come ??
No
did u know Principle of this test
Mem plzz give me which article are used?
What do you mean ?
Ji sir ham
Mam I am from kerala
Please put english substile please
Video is also available in English, please check description box
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Chup learn hindi saar
Urine sample zayda liye hain apnay , its 8 drops or 1/2 ml if urine
Its qualitative not quantitative
Glucose solution kaha milayga
Glucose ko hi mix kar lijiye
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Watching this 20 mins before exam 😂. Thank you 🙏🏻😊
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Malayalathil viduka
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If the sugar is present it's colour will be orange
Kon kon exam k pahlay din padne aya hai
Main 🙂
Ch bsc zoology
READ BOOK 1st dont go wrong
not correct . 5 ml reagent and 8 drops of urine is correct method
Its not quantitative sir, its qualitative demonstration
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But it's looking like orange color 😢
In camera colour may be variable
Kon 2024 exam ke liye dekh raha 😂