What does it take to kill a bacterial spore? Petri dish timelapse

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  • เผยแพร่เมื่อ 23 ธ.ค. 2024

ความคิดเห็น • 1.5K

  • @francescobertelli847
    @francescobertelli847 5 ปีที่แล้ว +5162

    "Everything was killed!"
    *LOUD HEAVY METAL MUSIC PLAYING*

    • @Torch314159265
      @Torch314159265 5 ปีที่แล้ว +108

      Made me think of a Doom level for a moment.

    • @dandanthedandan7558
      @dandanthedandan7558 5 ปีที่แล้ว +27

      Reminds me of the hydraulic press channel intro

    • @mr.waffentrager4400
      @mr.waffentrager4400 5 ปีที่แล้ว +2

      I was going to say that...

    • @wartman1006
      @wartman1006 5 ปีที่แล้ว +18

      Death note almost

    • @aceshadowins1310
      @aceshadowins1310 5 ปีที่แล้ว +5

      Torch314159265 more like death note

  • @schizophreniagaming1187
    @schizophreniagaming1187 5 ปีที่แล้ว +3837

    "Everything was killed!"
    0.01% of bacteria: *Laughs microscopically *

    • @cringeusername9961
      @cringeusername9961 5 ปีที่แล้ว +146

      The rest 0.0001% that you haven talked about:am i a joke to you

    • @dandanthedandan7558
      @dandanthedandan7558 5 ปีที่แล้ว +9

      @Kaya Beautymantra It can also be the 0.0001 that is not included in the 0.01

    • @brian5619
      @brian5619 5 ปีที่แล้ว +53

      DANDAN THE DANDAN that makes no sense. Was your math taught by your gym reacher?

    • @kevinjhoncadiz2342
      @kevinjhoncadiz2342 5 ปีที่แล้ว +7

      @@brian5619 I know right?😂 We're talking about odds here and he makes no sense😂

    • @dandanthedandan7558
      @dandanthedandan7558 5 ปีที่แล้ว +5

      @@brian5619 No, I meant like if there's a hundred boxes, you can have three groups: 10 red boxes, one green box, and the remaining 89 that is unclassified. That's what I meant. Also, stop being rude you kid.

  • @CannotBeVerified
    @CannotBeVerified 5 ปีที่แล้ว +3793

    Interested to see the effect of alcohol or antiseptic cleaning products

    • @Jeeeeer
      @Jeeeeer 5 ปีที่แล้ว +477

      I can't provide the exact statistics because that would require indepth research into that, but I can tell you that even 70% alcohol is not trusted to sterilize surfaces, only disinfect. However at 90% ethanol bacterial tends to encyst and not die.

    • @CannotBeVerified
      @CannotBeVerified 5 ปีที่แล้ว +84

      @@Jeeeeer woahh but how to clean and kill tho. Crazy

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +756

      Yea they will survive through a lot of cleaning products.

    • @Jeeeeer
      @Jeeeeer 5 ปีที่แล้ว +385

      @@CannotBeVerified trust me, if you've been disinfecting (not sterilizing) surfaces all this time and have not fallen sick all the time, there's nothing to worry about. Less than 1% will hurt you, and there are many beneficial bacteria out there that help us too. I know how scary it seems that some bacteria do not get killed permanently, but this is more of an issue of labs and hospitals. Like the video shows, autoclaving is a routine in hospitals and labs where they are working wih the more dangerous strains. Nothing to worry too much about.

    • @elalogar7340
      @elalogar7340 5 ปีที่แล้ว +28

      ​@@sci-inspi Bleach included?

  • @dandanthedandan7558
    @dandanthedandan7558 5 ปีที่แล้ว +4421

    Oh come on using an autoclave is like bringing a nuke to a knife fight

    • @wormwoodbecomedelphinus4131
      @wormwoodbecomedelphinus4131 5 ปีที่แล้ว +462

      Increased thermal transfer due to higher pressure.
      Yeah. Not a fucking chance.

    • @NotSoCrazyNinja
      @NotSoCrazyNinja 5 ปีที่แล้ว +264

      There is a non-zero chance at some pathogen evolving to survive such harsh conditions. Very unlikely, but the chance is there. There already seems to exist living things that can survive an autoclave.

    • @dandanthedandan7558
      @dandanthedandan7558 5 ปีที่แล้ว +52

      @@NotSoCrazyNinja yeah it's called "next to impossible"

    • @tolentinoalexam.7262
      @tolentinoalexam.7262 5 ปีที่แล้ว +116

      @@NotSoCrazyNinja I mean it's true though, some bacteria CAN survive harsh conditions. They're called "Archaebacteria".(Please don't call me a nerd for this because I literally forgot what it was called though my teacher just taught this some days ago.)

    • @flamed_bars7299
      @flamed_bars7299 5 ปีที่แล้ว +32

      Tolentino, Alexa M.
      You’re not a nerd. I already knew that, but good observation

  • @kingseekerbackup3085
    @kingseekerbackup3085 5 ปีที่แล้ว +2802

    E. Coli: *Infects humans and probably causes death*
    Also E. Coli: *Dies to almost anything*

    • @anduro7448
      @anduro7448 5 ปีที่แล้ว +718

      *The glass cannon*

    • @PauaP
      @PauaP 5 ปีที่แล้ว +158

      @@anduro7448 Hay thats a pretty good name for E Coli.

    • @elie464
      @elie464 5 ปีที่แล้ว +199

      Well to be fair a human would probably die in the same conditions. They're just like us~

    • @albapantheratigris6071
      @albapantheratigris6071 5 ปีที่แล้ว +143

      I mean that’s why people at the CDC jump off of buildings when an E.Coli outbreak happens. Wash your damn hands, especially after you touch your poonanards, before you serve someone at a restaurant.

    • @kingseekerbackup3085
      @kingseekerbackup3085 5 ปีที่แล้ว +59

      @Jesus Christ whats your point?

  • @NoobAlike
    @NoobAlike 5 ปีที่แล้ว +1963

    Autoclave: I am inevitable ...

    • @Allplussomeminus
      @Allplussomeminus 5 ปีที่แล้ว +80

      Even the name sounds badass

    • @sundeww
      @sundeww 5 ปีที่แล้ว +17

      guy with chainsaw: and i... am going to destroy you

    • @Inidentificado
      @Inidentificado 5 ปีที่แล้ว +66

      And I..
      am...
      Prion Man

    • @rahmatbanua7009
      @rahmatbanua7009 5 ปีที่แล้ว +7

      Inidentificado i see what you did there. Protein man

    • @SpicyGregPowers
      @SpicyGregPowers 5 ปีที่แล้ว +6

      and I'm pepsi man

  • @6Twisted
    @6Twisted 5 ปีที่แล้ว +2229

    Damn, 2 minutes of UV light was enough to kill most of them where as 10 minutes of boiling hardly affected the spores. I didn't realise UV was so damaging.

    • @Kanal7Indonesia
      @Kanal7Indonesia 4 ปีที่แล้ว +259

      All hail the UV light!

    • @kellynolen498
      @kellynolen498 4 ปีที่แล้ว +109

      @@jomsies
      sunscreen is uv black face.......
      .........._|prove me wrong|_

    • @sharpen193
      @sharpen193 4 ปีที่แล้ว +79

      @@kellynolen498 well first of all sunscreen is white

    • @kellynolen498
      @kellynolen498 4 ปีที่แล้ว +166

      @@sharpen193 well its simple stuff is white because it reflects the visible light its uv blackface because on a uv camera or if you could see in uv it would show up as black

    • @bongotoucan7913
      @bongotoucan7913 4 ปีที่แล้ว +14

      Kelly Nolen r/wooosh

  • @ritacortez3883
    @ritacortez3883 5 ปีที่แล้ว +1988

    "Time to bring out the big guns! THE AUTOCLAVE!!!..."
    And the change in the music... AMAZING!!!
    EXCELLENT WORK!!!

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +139

      Lol I had to make it match the video.

    • @GoldenizedElite
      @GoldenizedElite 5 ปีที่แล้ว +10

      @@sci-inspi solo tabs?
      didn't know you also played metal lmao

    • @thraximundar2974
      @thraximundar2974 2 ปีที่แล้ว +3

      @@sci-inspi evidently shazam recognizes the music too.

    • @snowstarsparkle
      @snowstarsparkle 2 ปีที่แล้ว +2

      @@thraximundar2974 LMAO, I didn't know it could do that! And what a nice song name too XD

    • @Kenmanhl
      @Kenmanhl 2 ปีที่แล้ว +3

      I knew the autoclave would work the moment the lid was closed and it showed the autoclave with a confidant smile

  • @monsegeek
    @monsegeek 5 ปีที่แล้ว +763

    If B. Licheniformis is so resilient and given the fact that it can also infect humans, I wonder if it's antibiotic resistent as well.

    • @pulvenberg1709
      @pulvenberg1709 5 ปีที่แล้ว +34

      After some time... Most propably

    • @ryanchuabowen2045
      @ryanchuabowen2045 5 ปีที่แล้ว +101

      @@changweihsu bacteriophage*

    • @Liamb2179
      @Liamb2179 5 ปีที่แล้ว +234

      I found a paper where they tested the susceptibility of 90+ strains of B. Licheniformis in korea, most of the strains they found were on average susceptible to some antibiotics, but interestingly were naturally resistant to two types (chloramphenicol and streptomycin) and there were a lot of strains that appeared to have acquired resistance to erythromycin. So on the whole it appears to be mostly resistant to some antibiotics but not at an alarming rate, however any bacteria can become resistant to any antibiotic over time. I also learned that it likes to live in fermented soybean based food which are very common in korea! Apparently infections from it are quite rare.
      Ref: Do-Won Jeong et al. Antibiotic susceptibilities and characteristics of Bacillus licheniformis
      isolates from traditional Korean fermented soybean foods, LWT-Food Science and Technology DOI: 10.1016/j.lwt.2016.10.001
      (disclaimer: I spent maybe 5 mins on this so might not be accurate, also this was just the first paper I saw and I've never even heard of this journal so take this with a grain of salt)

    • @monsegeek
      @monsegeek 5 ปีที่แล้ว +55

      @@Liamb2179 Wow, really nice of you to take the time and look it up. Thank you!
      And yes, totally true that any bacteria can develop antibiotic resistance over time. Current projections predict that by 2050 resistent bacteria will cause more human deaths than cancer. That's just terrifying, if you ask me.

    • @minepose98
      @minepose98 5 ปีที่แล้ว +10

      @@monsegeek It's just slightly less terrifying when you realize that this is in part due to cancer deaths being replaced by resistant bacteria deaths. Or does that make it worse?

  • @simunator
    @simunator 5 ปีที่แล้ว +470

    omg they just won't die

    • @Alexander99602
      @Alexander99602 5 ปีที่แล้ว +132

      Autoclave : I would like to introduce myself

    • @lynx5327
      @lynx5327 5 ปีที่แล้ว +50

      besides E. coli that got fucked real hard

    • @Dover939
      @Dover939 5 ปีที่แล้ว +8

      @Lynx
      fuck you ecoli get fucked

    • @Just_Disco
      @Just_Disco 5 ปีที่แล้ว +4

      Autoclave : I’m about to end this man’s whole career

    • @tedchirvasiu
      @tedchirvasiu 4 ปีที่แล้ว +1

      ...until *heavy metal music starts playing*

  • @sunisol3058
    @sunisol3058 5 ปีที่แล้ว +401

    The guitar riff sounds like the death note soundtrack

  • @illegal_juicebox
    @illegal_juicebox 5 ปีที่แล้ว +285

    *T H E A U T O C L A V E*
    [evil laughing]

    • @Leekodot15
      @Leekodot15 5 ปีที่แล้ว +2

      (Barely-evil laughing)

    • @barsozuguler4744
      @barsozuguler4744 4 ปีที่แล้ว +4

      I never seen a autoclave before this video. This will be interesting

  • @natanezra8589
    @natanezra8589 5 ปีที่แล้ว +73

    B. licheniformis: why do i hear boss music?

    • @deborahol
      @deborahol 4 ปีที่แล้ว

      Where is this bacteria mostly found, is it a very common bacteria?

  • @andricluis
    @andricluis 5 ปีที่แล้ว +132

    im more surprised that the blue plastic holder is still normal after heated at 121C for 30 min

    • @wildcloud420
      @wildcloud420 5 ปีที่แล้ว +61

      its heat resistant polymer based plastic. You could drop it from six stories and it would be okay.

    • @notyou6674
      @notyou6674 5 ปีที่แล้ว +26

      @@wildcloud420 yes but what if you dropped it from 6 stories and it was on FIRE?

    • @wildcloud420
      @wildcloud420 5 ปีที่แล้ว +63

      @@notyou6674 then we would all die

    • @PauaP
      @PauaP 5 ปีที่แล้ว +1

      @@notyou6674 Big ass explosion would happen.

    • @dienosorpo
      @dienosorpo 3 ปีที่แล้ว +4

      Polipropilene is a tough plastic. I sterilize it a lot in my moms pressure cooker lol. It also reaches 121 °C, 15 PSI, and i sometimes do 1:30 hrs of sterilization time. And the plastic resist various sterilization cicles

  • @Thecarloful
    @Thecarloful 5 ปีที่แล้ว +113

    This is one of the coolest science channel in youtube, keep it up my friend!

  • @thermomeds
    @thermomeds 5 ปีที่แล้ว +224

    RIP e.coli. You Will be missed

    • @Marcos_Tired
      @Marcos_Tired 5 ปีที่แล้ว +62

      "You Will (not) be missed"

    • @haekly264
      @haekly264 5 ปีที่แล้ว +12

      he was like a father to me

    • @Vandarte_translator
      @Vandarte_translator 5 ปีที่แล้ว +8

      @@haekly264 I (didn't) love him like a son.

    • @PauaP
      @PauaP 5 ปีที่แล้ว +5

      Not really, I prefee my food to be Ecoli free tbh.

    • @sulejmaniapologist
      @sulejmaniapologist 5 ปีที่แล้ว

      An hero

  • @ramblinevilmushroom
    @ramblinevilmushroom 5 ปีที่แล้ว +91

    so.. an autoclave is a fancy pressure cooker?

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +71

      Pretty much.

    • @urielseptim910
      @urielseptim910 4 ปีที่แล้ว +13

      Mmmm... homemade e-coli in the morning tastes so good from the pressure cooker

    • @itsgrace717
      @itsgrace717 2 ปีที่แล้ว +4

      @@urielseptim910 yummy bacteria soup

    • @Anthomemes
      @Anthomemes 2 ปีที่แล้ว +4

      There's nothing better than protozoa cereal on Saturday mornings.

  • @gessyb936
    @gessyb936 5 ปีที่แล้ว +403

    One question, is B. Lichenformis infectious? To any organism? Also , lovely music!

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +266

      It can infect humans.

    • @stap0510
      @stap0510 5 ปีที่แล้ว +30

      @@sci-inspi But is an infection harmful to people?

    • @sadiev2778
      @sadiev2778 5 ปีที่แล้ว +152

      @@stap0510 Infections are always harmful. Immune response, stealing nutriënts, possibly producing toxcins.

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +186

      Yes the infection can harm humans.

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +258

      @@Chartmaster2099 I have a bachelors degree in biology. I am a lab tech at a community college. I set-up materials, supplies and equipment for college students in order for them to do experiments in their biology college courses. I also order all the materials and maintain the lab.

  • @trashuser8646
    @trashuser8646 5 ปีที่แล้ว +65

    Kills 99.9% bacteria
    0.1%: its time to reproduce myself

    • @kamhargrove8694
      @kamhargrove8694 3 ปีที่แล้ว +4

      Are you talking about the autoclave? Autoclaves kill ALL microorganisms.

  • @kirenireves
    @kirenireves 4 ปีที่แล้ว +125

    Were the tubes covered in the autoclave? How does that work?
    Also, after the UV test, how do the surviving microbes travel to the dead side? Air currents in the covered dish?

    • @sci-inspi
      @sci-inspi  4 ปีที่แล้ว +127

      The cap is vented, it allow air and steam to enter the tube. The microbes don’t travel in the uv test. That is were they were. Just not all of them died.

  • @anrie2623
    @anrie2623 5 ปีที่แล้ว +85

    Autoclave sounds like adventurer fantasy weapon

  • @edwchristian123
    @edwchristian123 5 ปีที่แล้ว +69

    E. Coli: didn't survived at many tests
    Sci- Inspi= *Time to bring out the big guns!*
    Also E. Coli= *:')*

  • @laboonplatoon9263
    @laboonplatoon9263 5 ปีที่แล้ว +73

    Please remind people to autoclave bacteria before disposing of it. I love DIY science but it’s super critical to teach proper sterilization and disposal techniques. Boiling bacteria and culturing it on agar, leading to cultures of a few CFU, could lead to the introduction of a dangerous strain into the environment.

    • @Frank-im2ku
      @Frank-im2ku 2 ปีที่แล้ว

      Sorry for my ignorance what does CFU means?

    • @jochembraad1760
      @jochembraad1760 2 ปีที่แล้ว +6

      @@Frank-im2ku colony forming units. So the few bacteria that survive the harsh condition are able to form many more bacteria and are now a new strain that is more adapted to a harsh condition and thus thus harder to kill

    • @Frank-im2ku
      @Frank-im2ku 2 ปีที่แล้ว +2

      @@jochembraad1760 thank you very much! Now i see why the op was worried about it.

    • @bitterlemonboy
      @bitterlemonboy 2 ปีที่แล้ว +1

      @@jochembraad1760 1. Take bacteria.
      2. Treat with harsh conditions.
      3. Take the colonies that survive and treat them with even harsher conditions...
      4. Rinse and repeat
      5. Create a super-bacteria that wipes out all of humanity

  • @hilalk-b
    @hilalk-b 2 ปีที่แล้ว +3

    Autoclave is my hero. The first thing I always do in a new lab is to learn how that exact autoclave works or is there a specificly assigned staff to use it and what is its schedule. Even thought I am scared to work in lab when autoclave is on. Sometimes I wish to have it at home.

  • @Gvozd111
    @Gvozd111 5 ปีที่แล้ว +69

    What about some antiseptics? Chlorhexidine, ethanol, hard liquor ?

    • @johnc7532
      @johnc7532 5 ปีที่แล้ว +13

      Берзин Григорий ethanol and hard liquor is the same thing. The only difference is that lab ethanol is denatured.

    • @Thematic2177
      @Thematic2177 5 ปีที่แล้ว +14

      @@johnc7532 - hard liquors are only about 40% alcohol by volume.

    • @easysyndicate
      @easysyndicate 5 ปีที่แล้ว

      ...

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +31

      They can survive a lot of antiseptics.

    • @YommyRawr
      @YommyRawr 5 ปีที่แล้ว +10

      @@sci-inspi what about those detergent that claim to kill 99.9% of germs ?

  • @Milkex
    @Milkex 2 ปีที่แล้ว +2

    I actually listened to Boiled Dirty Water on Spotify Discovery Weekly while I was building my Openflexure Delta Stage (microscope) without knowing it was you! I had seen the boiled dirty water video maybe two weeks before this and never added 2+2. You just got a sub for life!

  • @jhoxha
    @jhoxha 5 ปีที่แล้ว +78

    Autoclave: I have become death, destroyer of worlds .... or petri dish bacterial cultures in our case

    • @Jin-di9zv
      @Jin-di9zv 5 ปีที่แล้ว

      Both just deleted. Well, not really surprising for E.coli

    • @PauaP
      @PauaP 5 ปีที่แล้ว +2

      Well a petri dish becterial cultures can be considered "worlds" if you think about it.

    • @jhoxha
      @jhoxha 5 ปีที่แล้ว +3

      @@PauaP kinda reminds me of that scene in man in black where a whole alien colony lives in jays locker and whenever he opens it they chant Jay Jay Jay

    • @PauaP
      @PauaP 5 ปีที่แล้ว +4

      @@jhoxha Exactly. So in this sense, the Autoclave literally became death, destroyer of worlds.

  • @megsley
    @megsley 2 ปีที่แล้ว

    my dad does installation and maintenance on hospital autoclaves and other sterilization equipment - he really enjoyed this video! cheers!

  • @RayMak
    @RayMak 4 ปีที่แล้ว +10

    This is so much time and effort to make and so educational. Thank you! Didn't know some bacteria can be so tough!!

    • @icarokaue7334
      @icarokaue7334 4 ปีที่แล้ว +3

      How are you everywhere

  • @joesteadman343
    @joesteadman343 3 ปีที่แล้ว +2

    I like the music. At around 00:25 I felt like Kira was going to write my name in the Death Note.

  • @timothykock6799
    @timothykock6799 5 ปีที่แล้ว +14

    This is so fun to watch! I am studying this myself and I'm always very exited to see micro organisms in that way

  • @frederickboateng3574
    @frederickboateng3574 5 ปีที่แล้ว +17

    When I saw “Bring out the big guns “ I thought she was gonna bring out Lysol

  • @setlenbolstenderods1617
    @setlenbolstenderods1617 5 ปีที่แล้ว +17

    "I will put bacterial cultures for 30 mins"
    2.5 hours later...

  • @lumineaether1494
    @lumineaether1494 5 ปีที่แล้ว

    ok,this has to be the best channel i have seen right now

  • @saint0004
    @saint0004 5 ปีที่แล้ว +22

    Great choice of music

    • @den0616
      @den0616 5 ปีที่แล้ว +1

      i think he makes them on his own

  • @mysteriousarcanum2769
    @mysteriousarcanum2769 2 ปีที่แล้ว +2

    As someone who wants to be a microbiologist, this was very entertaining to watch.

  • @jsutien
    @jsutien 5 ปีที่แล้ว +50

    *microbes exist*
    Autoclave: "im about to destroy this man's career"

    • @Stogie1
      @Stogie1 5 ปีที่แล้ว +1

      Autoclave: "ima bout to end this man's whole species"

  • @ataplin3058
    @ataplin3058 3 ปีที่แล้ว +1

    Never in my life would I think of an auto-clave as badass until now!

  • @ec35317
    @ec35317 4 ปีที่แล้ว +12

    Great experiments! Thank you!
    This may be the reason why people who get Clostridium Dificile (which has spores) sometimes get RE-infected since bacterias with spores are hard to kill on surfaces.
    You used 2 minutes of UV light. How long under UV light would kill the B. Licheniformis completely?
    Also, you boiled only for 10 minutes. So how long using the boiling method would actually kill the B. Licheniformis completely?

    • @extatixz
      @extatixz 2 ปีที่แล้ว +3

      As someone who has been battling reoccurring cases of C.Dif for the past year and a half due in part to Ulcerative Colitis, I can confirm. It’s a bitch to get rid of.

    • @MyBoyNewton
      @MyBoyNewton 2 ปีที่แล้ว

      Boiling will not kill the spores. They don't metabolize and reproduce but they survive.

  • @hhthoj
    @hhthoj 5 ปีที่แล้ว +8

    You should make another test. Submerge them in WBC and see if they will survive macrophages.

  • @chevinramheet6858
    @chevinramheet6858 5 ปีที่แล้ว +20

    What will happen if you boil the test tubes of bacterial culture for 2.5 hours?

    • @nerodino5508
      @nerodino5508 5 ปีที่แล้ว +5

      What will happen if you put something really hard to kill (tardigrade?) inside the Autoclave for 25 hours though?

    • @trent800
      @trent800 2 ปีที่แล้ว +1

      @@nerodino5508 Tartigrades will be fine if they are in their tun state which can survive up to 150 C

  • @gavini3383
    @gavini3383 4 ปีที่แล้ว +2

    The background music at the start sounded like a death note soundtrack

  • @TreyPDB
    @TreyPDB 5 ปีที่แล้ว +17

    the music is really good

  • @olivegarden2137
    @olivegarden2137 4 ปีที่แล้ว +1

    I'm glad to have found this channel, not only is the content amazing but the music is just as good.

  • @hunterjensen8111
    @hunterjensen8111 5 ปีที่แล้ว +9

    That one tough microbe! It took a fancy scientific pressure cooker to kill it

  • @o0Avalon0o
    @o0Avalon0o 4 ปีที่แล้ว +2

    The music started and all I could think of was Death Note... Great show.

  • @aetherblackbolt1301
    @aetherblackbolt1301 2 ปีที่แล้ว +4

    If only 2 minutes under a UV lamp did that much, I wonder how good 10 minutes or even 30 minutes would do.

  • @TheSyntheticAbomination
    @TheSyntheticAbomination 5 ปีที่แล้ว +1

    E. Coli: *dies*
    B. Licheniformis: *Bitch how dare you still alive*

  • @esclapter
    @esclapter 5 ปีที่แล้ว +6

    Is the pressure in the autoclave used for actual sterilization purposes, like does high pressure actually harm the microbes by itself? Or is it only used to allow the temperature to go over 100 degrees?

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +8

      The pressure allow water solutions to get hotter than 100 degrees Celsius. This will kill microorganisms because some can survive at 100 degrees.

  • @unironicdoomer614
    @unironicdoomer614 4 ปีที่แล้ว +1

    It’s awesome that I now do this kind of lab work at university, trust me guys this is a lot more badass when you yourself are also testing bacteria.

  • @paraumbralin
    @paraumbralin 5 ปีที่แล้ว +25

    You're one cool dude.
    Also, poor E. coli

  • @jesper164a
    @jesper164a 5 ปีที่แล้ว +2

    So if i had some B. Licheniformis on my door handle or hands, how do i kill it by not putting my hand or door handle into a Autoclave for 2.5 hours?

    • @jesper164a
      @jesper164a 5 ปีที่แล้ว

      @@MaxG-jk8ty So how do i kill it if it's on the handle?

    • @martinerhard8447
      @martinerhard8447 5 ปีที่แล้ว

      If you have a metal doorknob just heat it with a torch

  • @dal.salvador
    @dal.salvador 5 ปีที่แล้ว +38

    This was really cool and informative.

  • @jaxonpontious8018
    @jaxonpontious8018 5 ปีที่แล้ว +1

    I think what you do here is super cool and your channel is very underrated for the neat shit you're doing

  • @Rhoxe
    @Rhoxe 5 ปีที่แล้ว +55

    E.coli are weak af, God gotta buff them up in the next update

    • @Cattus_Maximus
      @Cattus_Maximus 5 ปีที่แล้ว +24

      Then I ain't swimming in any pool.

    • @soyderiverdeliverybeaver8941
      @soyderiverdeliverybeaver8941 5 ปีที่แล้ว

      Tiermicro

    • @ao2415
      @ao2415 5 ปีที่แล้ว +3

      Rhoxe .C just main parasites and just farm exp inside a host noobs.

    • @tigershark8867
      @tigershark8867 5 ปีที่แล้ว +6

      The Anti-vaxx faction should be an easy target.

    • @Sectsoldier
      @Sectsoldier 5 ปีที่แล้ว

      Nah, let's just vault em until the next patch

  • @luciusn21
    @luciusn21 2 ปีที่แล้ว +1

    Watching your videos in my microbiology class. Love the music!

  • @isidoraedwards9615
    @isidoraedwards9615 5 ปีที่แล้ว +24

    so cool ☺️ i'm learning about biology on my own through the internet and your channel is awesome for this purpose 💕
    is there any way to effectively sterilize objects from spore forming bacteria other than the autoclave?

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +11

      Preventing them from growing is best, but they are hard to kill. Autoclaving is the best way to kill spores.

    • @isidoraedwards9615
      @isidoraedwards9615 5 ปีที่แล้ว +2

      @@sci-inspi i'll look more into the prevention of their growth thank you! 💗

    • @isidoraedwards9615
      @isidoraedwards9615 5 ปีที่แล้ว +1

      ​@Unique SP thank you 💗would boiling them for prolonged periods of time be effective as well?

    • @capertillar4634
      @capertillar4634 3 ปีที่แล้ว

      @@isidoraedwards9615 peepee

  • @sublimeade
    @sublimeade 4 ปีที่แล้ว +2

    Did you try essential oils?

  • @keyontaeg.6645
    @keyontaeg.6645 5 ปีที่แล้ว +6

    Simply exquisite. My interest in videos like this confirm my own nerdy nature. Thanks, & keep them coming.

  • @Brian_Vallejo
    @Brian_Vallejo 2 ปีที่แล้ว

    The guitar shredding built up the anticipation!

  • @user-fh5ov4tu2j
    @user-fh5ov4tu2j 5 ปีที่แล้ว +6

    The music kind of sounds like Death Note soundtrack, i love it

  • @ArchangelExile
    @ArchangelExile 2 ปีที่แล้ว +1

    When you said "big guns", I thought you were going to bring out some Lysol.

  • @jeevacation
    @jeevacation 5 ปีที่แล้ว +14

    In what way does B. lichenformis affect humans? Nice video btw :)

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +9

      It is possible for them to infect humans.

  • @V77710
    @V77710 2 ปีที่แล้ว +1

    Came for the experiment, stayed for the amazing guitar track

  • @haziq880
    @haziq880 4 ปีที่แล้ว +4

    Impressive video! Do you usually produce music or the video first?

    • @sci-inspi
      @sci-inspi  4 ปีที่แล้ว +4

      I edit the footage together first to see what kind of music I should make. So video and then music.

  • @whyareyoureadingmyname762
    @whyareyoureadingmyname762 2 ปีที่แล้ว +1

    Idk why but I find the pictures with "E. coli didn't survive." to be hilarious

  • @hogane2290
    @hogane2290 5 ปีที่แล้ว +3

    Your music is so great. This reminds me of Deathnote.

  • @drhgbiology
    @drhgbiology 4 ปีที่แล้ว

    Any chance of obtaining a transcript? This is great for my microbiology class.

  • @cmdrjackwhite5443
    @cmdrjackwhite5443 5 ปีที่แล้ว +8

    Looks like we all need to start cooking the autoclave way

  • @ajhproductions2347
    @ajhproductions2347 4 ปีที่แล้ว

    This is all soooo metal...that solo at the end is boss

  • @lasagna4layf132
    @lasagna4layf132 5 ปีที่แล้ว +35

    Mother: Doc my son has a bacterial spore infection what do we do.
    Doctor: We *AUTOCLAVE* him
    Mother: What does it do?
    Doctor: It boils and CRUSHES THE BACTERIA AND YOUR SON
    Mother:
    Son:
    Mother:
    Son:
    Police: Hi I'm officer John please come with me to the office Doc

    • @kami5198
      @kami5198 5 ปีที่แล้ว +1

      haha funny TH-cam guy

    • @akbarputraa7574
      @akbarputraa7574 5 ปีที่แล้ว

      @@kami5198 bro.

    • @kami5198
      @kami5198 5 ปีที่แล้ว

      @@akbarputraa7574 b r o

  • @Sannypowa
    @Sannypowa 5 ปีที่แล้ว +1

    If I went underground enough to replicate the conditions under which the autoclave operated, will it kill the bacterial spores?

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว +1

      Yup

    • @Sannypowa
      @Sannypowa 5 ปีที่แล้ว

      @@sci-inspi I wonder if we could artificially replicate the conditions in hospitals to kill superbugs in human bodies on lower temperatures

  • @EltaleLibrarian
    @EltaleLibrarian 5 ปีที่แล้ว +5

    The beginning of this music sounds like L's theme from Death Note, and I like it.

  • @MisterFds
    @MisterFds 2 ปีที่แล้ว +2

    Some spores are resistant to 121 c and you need to get up to 170-180. They are remarkable.

  • @aandreoli8961
    @aandreoli8961 5 ปีที่แล้ว +4

    No gamma rays :)?

    • @tropixMw2
      @tropixMw2 5 ปีที่แล้ว

      Vill do same as UV

    • @aandreoli8961
      @aandreoli8961 5 ปีที่แล้ว +1

      @@tropixMw2 I dont think so, gamma rays are much more penetrating and energetic, they would have the same effect as the autoclave

    • @loandx2074
      @loandx2074 5 ปีที่แล้ว

      yeah but where is he supposed to get gamma rays from? 😂

  • @Eric.Clay.
    @Eric.Clay. 5 ปีที่แล้ว +2

    How does this Autoclave pressure work? especially considering that the bacteria is inside tubes! what happens inside the machine?

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว

      The autoclave uses heat, steam and pressure to kill microbes. The test tubes have loose caps on, the steam can get inside.

    • @Eric.Clay.
      @Eric.Clay. 5 ปีที่แล้ว +1

      @@sci-inspi i see! thanks for explanation!
      I wish it was possible to see under a microscope how pressure and steam affect these spores as it happens.
      Maybe put a microscope in the Autoclave and record a video :D

    • @sci-inspi
      @sci-inspi  5 ปีที่แล้ว

      Lol I don't think that would be good for my microscope. But I can try to see what I can do.

  • @jhtang5441
    @jhtang5441 5 ปีที่แล้ว +4

    Who initially thought the Big Guns was 99.99% hand sanitizer?

  • @gregoryallen2748
    @gregoryallen2748 4 ปีที่แล้ว +1

    Your channel is f*king awesome. Seeing these would of made class much more interactive and pertinent to real world than a text book image.

  • @ninjahunter6925
    @ninjahunter6925 4 ปีที่แล้ว +3

    I thought it would be like “let’s put different antibiotics in the culture” but you went for the nuke autoclave lol

  • @Agilku117
    @Agilku117 5 ปีที่แล้ว +1

    TH-cam sure know what their user needs. I have an exam on microbiology next week and TH-cam recommend me this video. Thanks TH-cam. Good vid tho.

  • @wuznab5109
    @wuznab5109 5 ปีที่แล้ว +6

    So all I need to do is jump into an autoclave.

  • @Hidden_Lizard
    @Hidden_Lizard 4 ปีที่แล้ว +1

    What if you did 10 minutes under the UV light?

  • @TonYnoT117
    @TonYnoT117 4 ปีที่แล้ว +3

    Would love to see how effective an ozone generator would be against bacteria and viruses.. video hint ;)

  • @19billdong96
    @19billdong96 5 ปีที่แล้ว +2

    “Brought to you by... Autoclave!”
    *start blasting heavy metal*
    Super cool video, loved it

  • @TheDevilockedzombie
    @TheDevilockedzombie 5 ปีที่แล้ว +10

    I love these videos. Absolutely interesting and the music is always superb.

  • @asmodeus20
    @asmodeus20 2 ปีที่แล้ว +2

    I'm a microbiology teacher and a metalhead. I use your video to intro my students to the autoclave, and turn that sucker up for the shredding🤘

    • @sheriffwolf736
      @sheriffwolf736 2 ปีที่แล้ว

      Would love to have you as my micro teacher instead of the ones that I have now.

  • @Sorceress_of_Minds
    @Sorceress_of_Minds 5 ปีที่แล้ว +7

    This was very interesting to watch!
    The microscopic world is being forgotten most of the time by the majority of people although it exists around and within us at all times and we are inseparable from it.
    I'm learning from your videos, you show great content!
    Also, I really like your music. It fits perfectly and is well composed. Keep up the good work!

  • @fixitralphltd4988
    @fixitralphltd4988 ปีที่แล้ว +1

    Love the music choice when pulling out the big guns for total distruction on all life forms! 🤘😁

  • @justgame5508
    @justgame5508 5 ปีที่แล้ว +3

    5:48 AND ITS SWEET, SWEET, SWEET VICTORY, YEAH

  • @DefaultKnight
    @DefaultKnight 2 ปีที่แล้ว

    This man is going to fuck around and release a new variant

  • @armstrong2450
    @armstrong2450 5 ปีที่แล้ว +3

    Music: mass zombie massacre
    Reality: killing *Bacteria*

    • @GygasDistruttore
      @GygasDistruttore 5 ปีที่แล้ว

      Zombie apocalipses are caused usually by bacteria. So it is fitting.

  • @RenVajr
    @RenVajr 4 ปีที่แล้ว +1

    I am interested to see if they will survive any alcohol of hand sanitizers.

  • @bryanmartinez6600
    @bryanmartinez6600 5 ปีที่แล้ว +5

    Don't worry I got the Hand Sanitizer that kills 100% of germs from area 51.

  • @darrellboggess4954
    @darrellboggess4954 2 ปีที่แล้ว +1

    i don't see how the 15 psi did anything when the samples were sealed in the vials protecting them from the pressure outside the vials...perhaps it was taking the temperature above boiling for a longer duration that actually did the trick...first time they were boiled for 10 minutes (again in a sealed container) with equal temperature inside vials being less than 10 minutes... the second time they were essentially boiled for 30 minutes up to 2 1/2 hours...more than long enough for temps inside to equal temps outside the vials

    • @sci-inspi
      @sci-inspi  2 ปีที่แล้ว

      The caps on the tubes are vented, they allow air in and out. So the pressure acts on the liquid. The increased pressure allows the liquid to reach 121 degrees Celsius and kill spores. Water at normal pressure can only reach 100 degrees Celsius before it boils off into steam.

    • @darrellboggess4954
      @darrellboggess4954 2 ปีที่แล้ว

      @@sci-inspi kinda overkill when a simple halogen heat lamp can get much hotter than that at normal air pressure...putting your hand close to some modern headlights can instantly burn you

    • @sci-inspi
      @sci-inspi  2 ปีที่แล้ว

      @@darrellboggess4954 It has to be overkill to kill spores. They are tough buggers.

  • @billetede2peso113
    @billetede2peso113 5 ปีที่แล้ว +4

    Me: E. Coli
    the guy she told me to not worry about: B. Licheniformis

  • @kurostyx9124
    @kurostyx9124 5 ปีที่แล้ว +2

    so autoclave is basically a giant pressure cooker?

  • @Naudia93
    @Naudia93 5 ปีที่แล้ว +3

    Brad’s Wife would have killed that bacteria first try. 11 years of service dude.

  • @saosaqii5807
    @saosaqii5807 2 ปีที่แล้ว +1

    It is odd how spores work like that
    You would think the heat would still kill the spores but it lived even though it's non spore self died.
    It just doesn't make sense how the spores are more immune when all they have is just an extra hard shell.

  • @VoidHalo
    @VoidHalo 5 ปีที่แล้ว +3

    I'm really glad you used an actual UVC lamp instead of just some LEDs or something. I would have lost it if you used LEDs. This was a really great video. I don't usually like it when people just put text on the screen and play music over the video in lieu of an actual narration, but for the most part I found the music was fairly non-invasive. Until the end, of course. :)

  • @bowloffruitloops2952
    @bowloffruitloops2952 5 ปีที่แล้ว +2

    4:35 in the top middle there’s a tiny speck that survived