You are the genetics version of The Organic Chemistry Tutor on youtube. I'd never make it without people like you guys patiently and clearly breaking down all these concepts
Totally saved the day! I have an exam tomorrow and I had a lot of doubts on this topic. You’ve managed to solve pretty much all of my problems. Thanks a lot!
Thanks for the video! I like how organized your layout is.. You definitely cleared things up for me! I'll be checking out your other videos for future help :)
This video is awesome. A question on Eukaryotic Transcription. Are there any articles out there on theories and specualtions of how it works. Please if you know of any article do share. Would like to read up on what scientist think. Thanks
You're the best I was stuck with this from the morning and you saved me thank u, can I ask something is the loop will stay like that when the rna polymerase release the new rna strand ?
Moe Abu Omar Thanks, dude! I'm happy to help! If you're interested in having me tutor you, that could very well be possible. Check out my website: www.MoofUniversity.com and click on the "Tutoring" tab for more info, and email me at MoofUniversity@gmail.com if you really are interested in my tutoring services.
Hey I was hoping maybe you could answer these two short questions for me: 1. For Rho-independent termination, does the Poly-A tail following the second inverted repeat play a role in this termination at all? 2. For Rho-dependent, since its still DNA, wouldn't this strand still have inverted repeats and therefore cause the rho-independent termination? I'm just confused as to why it would choose to go through rho-dependent over the rho-independent process? Thanks! Once again, awesome video!
Hi, i have a question. why are both types of termination necessary the Rho dependent and independent. i get the difference between the two but not the biological significance in there being two different ones.
I just have a question: - Is the portion in the hair loop translated in the same way as the rest of the mRNA? And awesome vid btw! You explained everything in a way that's actually understandable.
I this it's because the hair pin loop is heavy (being made of G&C's which are triple H bonded) and after the loop is formed there is a region a A&U's and since they only have two hydrogen bonds, they are weaker and it is weighted down by the loop and dissociates.
please tell the affect of below mutations on RNA polymerase and transcription? Mutations on pribnow(-10 region) a-change last T residue of pribnow sequence to A b-change first A residue of pribnow sequence to T residue Mutations on -35 sequences c-change second T residue of -35 sequences to A residue d-change C residue of -35 region to A residue
Awesome video! But what is about the poly-U sequence at the 3'-End of the synthesised RNA? Isn't that an important factor of rho-independet termination?
Thank you for the video, however, you did not mention that BOTH rho-depend and in-depended form hairpin loop, and did not mention the rut site. maybe you need to add those!!
Faith Zaker Where are you getting that information from? Please provide a source. I've never come across anything that said rho-dependent termination involves a hairpin loop.
I think it's because U-A bonds are weaker than C-G bonds, So if there's alot of A-U bindings/sekvenses, it will have a lower affinity to the DNA in the polymerization process. So it will detach much easier than if it was alot of C-G involved.
The way you explain it makes perfect sense. The way my Professors in Rostock, Germany explain this, is just twisted, boring and nonsenical. Also, compared to English, German is such a messy and inefficient languages. Even if German is my mother tongue, I never get what the fuck they are taking about when they explain scientific stuff.
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You are the genetics version of The Organic Chemistry Tutor on youtube. I'd never make it without people like you guys patiently and clearly breaking down all these concepts
Thank god, you're video really cleared the whole process up for me, especially for Rho-independent termination. Thanks!
thank you, you're helping me 9 years later
Totally saved the day! I have an exam tomorrow and I had a lot of doubts on this topic. You’ve managed to solve pretty much all of my problems. Thanks a lot!
you're a life saver, thank you so much for taking the time to make these!
the only video that helped me, thank you so much for making this.
you made this 9 years ago and its still helping people
I only hope you realize how much reading and re-reading you are saving me by having this video. Very hard concept for me. Thank You. You Rock!
BAdBrAd Lol. Reading gets pretty lame. Videos all the way! Thanks for the kind words, dude!
I watched some of your vids and they were really useful. Thanks :D
Great explanation what you're doing is really helpful especially to some of us with exams... thanks
Great videos!! Very well set up and easy to understand :)
Thanks for the video! I like how organized your layout is.. You definitely cleared things up for me! I'll be checking out your other videos for future help :)
Awesome! I'm happy to hear that! :]
was looking for a video to explain how rho protein works and this was perfect. thanks!
You're my Hero !!!!! You taught me so much , You're the Best online GURU(Teacher) I ever had . Namastey , and loads of Love from India. Cheers!!!
namaste 🙏
lol...namaste
xD
Thank you. This is what I have been looking for 👍💐
You are a skilled teacher. Thank you Moof!
Thanks, dude! Sure thing!
you have a special way of reaching, thanks alot dude
Thank you for your video, it's very clear! I'll pass an exam for PhD fellowship and it's about termination in prokaryotes ^^
This video is awesome. A question on Eukaryotic Transcription. Are there any articles out there on theories and specualtions of how it works. Please if you know of any article do share. Would like to read up on what scientist think. Thanks
also a KEY feature of Rho independent termination is a run of ~6 U residues right after the GC loop
Amazing tutor🙏🏽
You're the best I was stuck with this from the morning and you saved me thank u, can I ask something is the loop will stay like that when the rna polymerase release the new rna strand ?
thank you! this video really helped me understand the hairpin loop!
Your awesome dude, you're getting me through med school :D I wish you could tutor me :/
Moe Abu Omar Thanks, dude! I'm happy to help! If you're interested in having me tutor you, that could very well be possible. Check out my website: www.MoofUniversity.com and click on the "Tutoring" tab for more info, and email me at MoofUniversity@gmail.com if you really are interested in my tutoring services.
I might just do that. Thanks dude
Moe Abu Omar Sure thing, yo!
@@moemad001 Hey Moe! I noticed you said you were in med school 5 years ago. Are you done now?
How does the hairpin loop signal to the RNA polymerase?
Can we say that inverted repeats form a palindromic sequence similar to that of palindromic recognition site for the restriction endonuclease ?
Thank you so much ! You are amazing !!!!!!!!!!! Wish you were here in South Africa
concise and informative! Thank you
Hey I was hoping maybe you could answer these two short questions for me:
1. For Rho-independent termination, does the Poly-A tail following the second inverted repeat play a role in this termination at all?
2. For Rho-dependent, since its still DNA, wouldn't this strand still have inverted repeats and therefore cause the rho-independent termination? I'm just confused as to why it would choose to go through rho-dependent over the rho-independent process?
Thanks! Once again, awesome video!
OMG! you are amazing! thanks a million
Hi, i have a question. why are both types of termination necessary the Rho dependent and independent. i get the difference between the two but not the biological significance in there being two different ones.
great video!
GREAT VIDEO.....WAS SO CLEAR
Thank you! ❤
It is very clear, thank you
Woow thank you so much, this is amazing
thanks
Awesome!! Thanks so much
Thank you!!!
Thank you...
Thank you so much
your the boss thank you 🌸
How does NusA (enzyme) is involved in this process?
How does the hair pin loop trigger the rna polymerase to fall off?
Thank you very much this was much better than how the book explained it!!!
You're welcome very much! I'm glad the video helped!
OMG i love you sir
this video saved me from the hell of biology 1a03 in mcmaster...
SJ Choi Lol. Awesome. I love you too, whoever you are. Haha.
I just have a question:
- Is the portion in the hair loop translated in the same way as the rest of the mRNA?
And awesome vid btw! You explained everything in a way that's actually understandable.
Yes
GC base pair are more stable mainly because of their base stacking interactions and not because they form 3 hydrogen bonds.
I at last understood transcription through this video!!! very well explained.
Payal Rao SWEET! Thanks! I'm happy to have helped!
Good job
That was great, thank you!
You're welcome! Glad ya liked it.
What about the terminator loop actually makes RNA polymerase disassociate?
I this it's because the hair pin loop is heavy (being made of G&C's which are triple H bonded) and after the loop is formed there is a region a A&U's and since they only have two hydrogen bonds, they are weaker and it is weighted down by the loop and dissociates.
thank you!!!!
please tell the affect of below mutations on RNA polymerase and transcription?
Mutations on pribnow(-10 region)
a-change last T residue of pribnow sequence to A
b-change first A residue of pribnow sequence to T residue
Mutations on -35 sequences
c-change second T residue of -35 sequences to A residue
d-change C residue of -35 region to A residue
this is the old model fro Rho-dependent termination and in the new model Rho is actually bound to RNA polymerase
Awesome video! But what is about the poly-U sequence at the 3'-End of the synthesised RNA? Isn't that an important factor of rho-independet termination?
Oh yea!
thank you
شكرا جزيلا :)
Good video! But I still got to learn termination in eukoryotic cells :( No matter the 'we don't know'
ohh man you are making the world easy.... (y)
Lol. Awesome.
Awesome video. Your template strand has T in it. Should those be U instead because it is RNA?
Thomas McDonald The template strand is a strand of DNA. So, no. It is correct as is.
Good
5' TATA 3' box is on the coding strand
Actually I still have no idea why the formation of stem-loop can stop the transcription.
Thank you for the video, however, you did not mention that BOTH rho-depend and in-depended form hairpin loop, and did not mention the rut site. maybe you need to add those!!
Faith Zaker Where are you getting that information from? Please provide a source. I've never come across anything that said rho-dependent termination involves a hairpin loop.
Genetics A Conceptual Approach by Benjamin A. Pierce
Faith Zaker Which edition? And can you quote it, exactly? If so, please note the page number as well.
4th edition and the fifth edition as well. but 4th edition page 363 figure 13.14
the hairpin loop on the rho-depended works to slow down the RNA poly so the rho can catch up to it.
Doesn't RNA polymerase move from 5' end to the 3' end?
Ollie Babes RNA polymerase synthesizes RNA 5'-->3', but reads the template strand 3'-->5' in order to do that. I hope that makes sense.
what about termination in eukaryotes ?
merryl mémé Honestly, I'm not entirely sure. I should probably learn it and make a video about that. Lol. Thanks for mentioning it.
+Moof University Look up the Eukaruotic transcription termination torpedo model, if you didn't already.
why does the hairpin loop end with uracils?
zeromethanez I'm not sure I understand your question. What do you mean?
I think it's because U-A bonds are weaker than C-G bonds, So if there's alot of A-U bindings/sekvenses, it will have a lower affinity to the DNA in the polymerization process. So it will detach much easier than if it was alot of C-G involved.
You forgot about the idea of palindrome, the inverted repeats are like a palindrome. But great video
The way you explain it makes perfect sense. The way my Professors in Rostock, Germany explain this, is just twisted, boring and nonsenical.
Also, compared to English, German is such a messy and inefficient languages. Even if German is my mother tongue, I never get what the fuck they are taking about when they explain scientific stuff.
Lol. I'm just glad you found the material makes sense now. Thanks for watching!
Thanks. Nice job with all 6 parts- Med student.
yea man. if the scientists dont know, then we for sure wont know lol
thanks