@prathyusharns9192. You should be able to acquire morphometric measurements if you know the scaling for your images, otherwise all measurements you get will be in pixels.
Hi there. Unless you want your measurements to be in pixels, your images should have the correct scaling information. If you need intensity measurements, scaling is not necessary.
Hi @Navnath. Are you referring to the image in 1:13 of the video? It’s an image of a stage micrometer (a slide with a scale). If you don’t have one, you can use a grid slide. If your sample is large in scale (like mm or inch), take an image of a ruler with your sample.
Dear Johanna, thank you, it helped me so much.
How to do caliberation for nuclear morphometry
@prathyusharns9192. You should be able to acquire morphometric measurements if you know the scaling for your images, otherwise all measurements you get will be in pixels.
Is scaling & spatial calibration necessary to measure both intensity and physical things, such as diameter, length, and so on?
Hi there. Unless you want your measurements to be in pixels, your images should have the correct scaling information. If you need intensity measurements, scaling is not necessary.
Thank you for getting back to me so quickly. I should start analyzing my image while watching your video. @@johanna.m.dela-cruz
how to get this scale image
you have used in calibration
Hi @Navnath. Are you referring to the image in 1:13 of the video? It’s an image of a stage micrometer (a slide with a scale). If you don’t have one, you can use a grid slide. If your sample is large in scale (like mm or inch), take an image of a ruler with your sample.
@@johanna.m.dela-cruz ok thanks will try to take picture