@@MedicalLabLadyGill i am still confused didn't get it how can a fibrin big clot appears in gel clot activator tube please explain it again because after blood collection when we put the tube for clotting for sometime after it's done clotting and we centrifuge it then how does the clot appears in the tube what is it ?
I will conduct a clinical trial as a site coordinator and one of my job is that I'm obliged to centrifuge and transfer the blood samples to the tubes seperately. Lab Kits will be sent but I know there will be only pipets and Sst tubes to collect the serum not the sticks which u have showed and used in video. Do I have to wait to plasma to be clotted and how can I be sure to collect all serum but not plasma because only serum is needed to analyze.any advise? Thank you.
OnurSevenDoğan This is a really great question! Thank you for asking. The Serum Separator Tube (SST) contains a clot activator and separator gel. The tube should be inverted about 5 times immediately after venipuncture. Centrifugation will then separate the cells from the liquid and put the gel separator in place. The clot I pulled out of this tube is not a frequent occurrence. Usually, tests performed on serum should not be affected if the liquid portion is still plasma.
Is this "stick" method the same as using the beads to get rid of the fibrin clot? Other than promptness, are there other benefits to using the stick method vs beads?
Time for blood in SST tubes to coaggulate you have to do inverting fo 5 time to insure the blood mixed with the Clot activator so it take 10 to 30 mints to clot so leave the tube standing for the same time, if you do it to fast you will end up with plasma Clots in the serum, the speed for centrifugeing is 3500 Rpm or (1.300g) for 6 to 10 mints.
HELLOW i know that plasma have a clotte so why you say that differant between plasma and serum that plasma is the liquid portion of blood before its clotted ??
Plasma should not have clots to be called plasma. To obtain plasma you need to pour blood into an anticoagulant tube (such as a hematology or clotting-timing tube). After mixing propperly, the liquid part of the blood that contains coagulation factors is inhibited and after separation of cells and liquids by means of centrifugation, you end up with plasma in the supernatant. Serum is what you obtain if you draw blood and you pour it into a tube without anticoagulant, and therefore the blood clotts naturally, or you pour it into a clotting-activator tube and clots form faster. Then you run a centrifugation step and the cells and clots go to the bottom, then you have serum at the supernatant.
No need for all of this, Just transfir the whol serum withe the clotted plasma to a SST and do centrifugeing 2500Rpm for 3 min then you will have serum with no cloted plasma
I work for problem resolution at a huge lab and this cured my curiosity for sure. I always thought that lump was just part of the gel
I remember being confused by this as a student. So glad to get the information out there!
@@MedicalLabLadyGill i am still confused didn't get it how can a fibrin big clot appears in gel clot activator tube please explain it again because after blood collection when we put the tube for clotting for sometime after it's done clotting and we centrifuge it then how does the clot appears in the tube what is it ?
Very cool!
Nice and interesting explanation I love you mam 🐱
What if I were to just spin it down a second time without stirring without the applicator sticks?
sst tube gel is danger if someone eat ❓😭 please answer 😭
I will conduct a clinical trial as a site coordinator and one of my job is that I'm obliged to centrifuge and transfer the blood samples to the tubes seperately. Lab Kits will be sent but I know there will be only pipets and Sst tubes to collect the serum not the sticks which u have showed and used in video. Do I have to wait to plasma to be clotted and how can I be sure to collect all serum but not plasma because only serum is needed to analyze.any advise? Thank you.
OnurSevenDoğan
This is a really great question! Thank you for asking.
The Serum Separator Tube (SST) contains a clot activator and separator gel. The tube should be inverted about 5 times immediately after venipuncture. Centrifugation will then separate the cells from the liquid and put the gel separator in place. The clot I pulled out of this tube is not a frequent occurrence. Usually, tests performed on serum should not be affected if the liquid portion is still plasma.
Is this "stick" method the same as using the beads to get rid of the fibrin clot? Other than promptness, are there other benefits to using the stick method vs beads?
Honestly, I have never used beads. Not sure I can answer your question.
After adding sample to tube how much time i can let it before centrfugation and the time of centrfugation and speed?
Time for blood in SST tubes to coaggulate you have to do inverting fo 5 time to insure the blood mixed with the Clot activator so it take 10 to 30 mints to clot so leave the tube standing for the same time, if you do it to fast you will end up with plasma Clots in the serum, the speed for centrifugeing is 3500 Rpm or (1.300g) for 6 to 10 mints.
HELLOW i know that plasma have a clotte so why you say that differant between plasma and serum that plasma is the liquid portion of blood before its clotted ??
Plasma should not have clots to be called plasma. To obtain plasma you need to pour blood into an anticoagulant tube (such as a hematology or clotting-timing tube). After mixing propperly, the liquid part of the blood that contains coagulation factors is inhibited and after separation of cells and liquids by means of centrifugation, you end up with plasma in the supernatant.
Serum is what you obtain if you draw blood and you pour it into a tube without anticoagulant, and therefore the blood clotts naturally, or you pour it into a clotting-activator tube and clots form faster. Then you run a centrifugation step and the cells and clots go to the bottom, then you have serum at the supernatant.
No need for all of this, Just transfir the whol serum withe the clotted plasma to a SST and do centrifugeing 2500Rpm for 3 min then you will have serum with no cloted plasma
or just run a centrifugation step again.