Class (38) = Plant Tissue Culture (Part 04) | Different Types of Plant Tissue Culture (Part 01)

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Plant Tissue culture
Plant tissue culture has a great significance in plant biotechnology, especially in the crop improvement programmes. The term tissue culture may be defined as the process of the in-vitro culture of explants (pieces of living differentiated tissues) in a nutrient medium under aseptic conditions
The following points highlight the top eight types of tissue culture. The types are:
1. Seed Culture 2. Embryo Culture 3. Meristem Culture 4. Bud Culture 5. Protoplast Culture
6. Root tip Culture 7. Anther/ Pollen Culture 8. Complete flower Culture 9. Ovary Culture
10. Hairy Root culture 11. Callus Culture 12. Cell Suspension Culture.
1. Seed Culture:
Seeds may be cultured in vitro to generate seedlings or plants. It is the best method for raising the sterile seedling.
2. Embryo Culture:
Embryo culture is the sterile isolation and growth of an immature or mature embryo in vitro with the goal of obtaining a viable plant.
3. Meristem Culture:
The apical meristem of shoots of angiosperms and gymnosperms can be cultured to get the disease-free plants.
4. Bud Culture:
Buds contain quiescent or active meristems in the leaf axils, which are capable of growing into a shoot.
5. Callus Culture:
Callus is basically more or less un-organised dedifferentiated mass of cells arising from any kind of explant under in vitro cultural conditions.
6. Cell Suspension Culture:
The growing of individual cells that have been obtained from any kind of explant tissue or callus referred to as cell suspension culture.
7. Anther Culture:
An important aspect of plant tissue culture is the haploid production by another culture or pollen culture which was first established
8. Protoplast Culture:
It is the culture of plant protoplasts i.e., culture of cells devoid of cell wall. Isolated protoplasts are usually cultured in either liquid or semisolid agar media plates.
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callus culture, suspension culture, single cell culture, organ culture. Ex-plant preparation
Methods of in-vitro plant propagation. Callus organogenesis and somatic embryogenesis
Strategies for production of transgenic plants with special reference to herbicide tolerance and insect resistance. Transgenic crops such as Bt cotton, Bt Brinjal, Round-up Ready Soyabean

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I am interested to get more practical infomation especially on buds, pollens, shoot tips, stems and leaves tissue culture.
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Meristem culture is 2 types root or shoot tip culture both are meristem culture

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Type and technique of plant tissue culture same or different????

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