Sir, i think one more point is important which is called gradient selection, it should equilibrate with mobile phase for some time called post run with appropriate solvent.
With regards to the Hot Water Purging. is there any guideline on how hot should the water be and also how long we should purge the whole system at what flow rate?
What is the criteria for online samples in RS analysis. That means how many hours before we can prepare sample solution. Is there necessary to inject blank between samples and bkt STD
Hi sir, my HPLC column so easy carryover after 3-5 run of sequnces after higher concentration STD. Can you please advise me how to clean the column ? Im pretty sure is carryover on the column.
sir diluent strength should not be more than mobile phase in which context?? it will came in stronger or weaker mobile phase or any other context. pls gave more light on this ??
Hi.. your video is really helpful for me can you make a video regarding integration parameters for HPLC and Impurities integration 🙏🙏🙏 Thanks in advance...
Sir, i think one more point is important which is called gradient selection, it should equilibrate with mobile phase for some time called post run with appropriate solvent.
With regards to the Hot Water Purging. is there any guideline on how hot should the water be and also how long we should purge the whole system at what flow rate?
Nice sir
What is the criteria for online samples in RS analysis. That means how many hours before we can prepare sample solution.
Is there necessary to inject blank between samples and bkt STD
May I know what OPA stands for?
Hi sir, my HPLC column so easy carryover after 3-5 run of sequnces after higher concentration STD. Can you please advise me how to clean the column ? Im pretty sure is carryover on the column.
sir diluent strength should not be more than mobile phase in which context??
it will came in stronger or weaker mobile phase or any other context.
pls gave more light on this ??
Sir point no. 5, in RPC use polar compound so why THF to use remove the non polar compound
Thanq sir
But in my case looks carryover but not carryover because lambda max of both totally different...what should I do
Thank u sir..Can I use diluent for needle wash....?
You can use
Yes u can use
Hi.. your video is really helpful for me can you make a video regarding integration parameters for HPLC and Impurities integration 🙏🙏🙏
Thanks in advance...