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- เผยแพร่เมื่อ 12 ก.ย. 2024
- Think you know how to use a centrifuge? Sure it's pretty straight forward, but a mistake can easily be made, resulting in serious damage or even injury.
So just remember these 4 points:
1) Use the right tubes
2) Balance the tubes
3) Secure the rotor lid
4) Check the settings
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lol @ 0:40 "this is not a washing machine"...
Yes. Seriously😂😂😂😂😂😂
Hehe😂
@Amorquies When you centrifuge your sample, the pellet will form on the side of the tube that is facing outwards. So, if you place the hinge on the outside, your pellet will form on the hinge side. This makes it easier to pipette out the supernatant without disturbing the pellet, as your pipette tip can rest against the other side of the tube (without being blocked by the tube lid) as you remove the liquid.
Try it out both ways and you'll see! =)
damn this tip is good
@luciferin1 Not balancing the tubes puts extra strain on the spindle. Depending on how imbalanced the tubes are, and how fast the rotor is spinning, you can badly damage the centrifuge....and even other objects (and people) around it. So always balance your tubes!
For many tabletop centrifuges, you can simply look at the tubes to see if they balanced. But for certain high speed centrifuges, you need to check their mass to make sure they are balanced.
Thanks to you, I'm more confident for my tomorrow experience at university ❤️
"this is not a washing machine this is actually a high speed centrifuge...you dont wanna mess with it", "if you follow these steps you will be fine, if you dont use the centrifuge correctly it may explode... *turns to camera man* wat its happen before!"" - hilarious, great video by the way! helped me with my bio revision, your accent is amazing it makes you sound so intelligent! If only my biology teacher was as funny as you it would be my favorite subject! :)
This video has really helped me with my work thank you very much. The video so much better than the rubbish you see pasted all over the net.
This is a very useful video. I am using it to prep our freshman students for their lab class. Credit given to the link & Dan, of course :-)
@allroundgirl123 When referring to centrifuges, usually the term centrifugal force is used. However, it is not a "real" force. Centripetal force is the "real" force which acts inwards, while centrifugal force appears to act outwards. I'm not an expert in physics, so if you want more details about this, check out your inbox as I just sent you some links (I can't insert them here) - the links also provide some good examples to help understand the concept :)
@8Avenger If you are worried that your protein may be coming out in the flow through, keep the flow through, and run a sample of it on an SDS-PAGE gel. If you see a band, then some of your protein leaked out through the membrane.
Also, just make sure that your filter's MW cut off isn't too close to your protein's molecular weight or else you run the risk of your protein going through.
It's really helpful,thanks a lot
Thank you for such informative video
Thank you . The tips are very helpfull !
First thing you need to know is that it's not a way to wash clothes faster. It... doesn't work out well. And the tube slots aren't for laundry detergent, like I thought....
Hey! Natani and Zen!
thanks for the video...it's really useful for me
Thanks bro you are An awesome dude And you deserves a like And a comment like this one alsof your centrifuges are very pretty And I want toen Marty one
This is really important. Please help me cause Im a beginner in using centrifuge. What are the parts of the centrifuge and the use of each? I really need help. This video help me btw but I need to know the parts. Thanks.
No they don't. As Dan mentions at 2:05 if you only have one tube, fill the other tube with water to the same volume.
Could you tell me, during the diafiltration process, how would you know if you were losing protein?
Why must I place the centrifuge tube with the hinge towards the outside of the centrifuge rotor?
Very helpfull ! thanks mate ;)
Thanks! Why do they make the centrifuges able to go too fast? For quick bursts of special tubes? In nuclear engineering labs a hacker slipped a thumb drive into an Iraqui computer control center and caused all their centrifuges to spin too fast and burn up the motors.
My prof sent me here. Nice video!
How does a centrifuge motor stop? what is the force that stops the motor spinning?
Michael Salsaa In my experience, when the centrifuge is unbalanced or improperly placed, the whole unit starts to shake violently. This can ultimately break the centrifuge.
@labtricks thank you so much! :) And I shall
How much is centrifuge
Thanks sir
Hi there, is it ok to use PET tubes for centrifugation?
Luis Ernesto Veli Jiménez In most cases, yes. But I would recommend checking with the manufacturer or the vendor.
Can anyone here educate me how to prepare prf( protein rich fibrin) to use in infected wounds as I m working at periphery n not much facilities r available here , we try to heal n help patients on our own within our resources...
I ve got a centrifuge old machine China made 800 centrifuge wth rpm:4000.n rcf:1790×g.capacity 20cc×5.
I also wanna know test tube's length its material n time to spin to retrieve max benefit from my produce.though I have general info frm watching diff u tube video but wanna get know frm experts as reading comments here I noticed that quite an experts in centrifuge technique are commenting here.
I wd really appreciate if anyone is to extend his knowledge to where it's really needed n be used productively..
Thanks in anticipation n prayers!
Thanks
What is the function of this? Im newbie in sciece and now i futher my study in matriculation
Shafriena Farhana In most cases, centrifugation is used for the separation of particles from a solution. For example; blood specimens are centrifuged to obtain serum/plasma. The serum/plasma can then be further analyzed in chemistry, coagulation, therapeutics, genetics, or blood banking.
Thank u 😊
Thankyou =)
Lol he looks like Bobby Lee
Next time put in a nice beatdrop and some genji potgs pls this was kinda basic
N o
T h i s i s n o t a w a s h i n g m a c h I n e
i dislike
HAVOCREPPINE4 okay.