northern blotting technique | northern hybridization | What is the principle of Northern blotting?

[@Yungfoxxjpn]

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[@animatedbiologywitharpan]

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You are saving me for my MCAT thank you

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[@khairerutuja1600]

Thank you , you explained really well 😊

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[@sayantansaha53]

Extraordinary explanation

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[@red.velvet.pirate6987]

Thank you for making this video, it was quite helpful!

[@animatedbiologywitharpan]

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[@somaraj1219]

No need to add accent, your content does not need it. good explaination.

[@animatedbiologywitharpan]

Thanks for the feedback....it would help me to improve in future

[@animatedbiologywitharpan]

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[@swagatamondal2518]

Thank you for this video

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience....don't forget to check out western and southern blot as well

[@swagatamondal2518]

@@animatedbiologywitharpan Ok Sir

[@_SakshiKumari-zk6vq]

Sooo helpfulll

[@animatedbiologywitharpan]

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[@doszlopleonard6031]

What is bro yapping about?

[@olfarayachi2980]

thank you u are amazing

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[@olfarayachi2980]

@@animatedbiologywitharpan ok you get it bro

[@k1morisato889]

good job man

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[@welcometosagnicksworld3894]

Dada from where you have learned your animation skills?

[@animatedbiologywitharpan]

It's acquired with time

[@nishat2000]

Hey, you are using different method for transfer mechanism. (Is it electric tranfer)

[@nishat2000]

Capillary is different. May you tell me the difference.?

[@animatedbiologywitharpan]

Capillary based method is ultra slow 🐌 . Hence electroblot. But the truth is no body in this world use Northern blot for research . Northern blot is alive only in text books. A cousin technique microarray is also dead. All of there heroic deeds are in textbooks.

[@blackicet2107]

Even microarray is dead ??
What replace it ?

[@nadja361]

thank you :)

[@animatedbiologywitharpan]

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[@brsznl]

With northern blot we can understand if the gene is expressed or not but can we make comments about the amount of the expression? İn another video i saw it was telling nothern is used for quantitive analiyses...

[@animatedbiologywitharpan]

Yes some quantification can be done but is a backdated technique, nobody use Northern blot these days . Alternative to Northern blot is real-time qPCR

[@brsznl]

@@animatedbiologywitharpan how the quantification was done? Ty for your answer

[@salmanfariz1052]

Thank u

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[@vidhyaminde6110]

Nice explanation 😊👍

[@animatedbiologywitharpan]

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[@DanteLopez-tg2si]

I thought a cathode was positive and an anode was negative

[@pinkisharma5646]

nice video will u pls make video on gene knockout mice

[@animatedbiologywitharpan]

Sure I will do that.......dont forget to subscribe my other channel th-cam.com/channels/4IpyopsGWSjaPACNTZLuqg.html

[@animatedbiologywitharpan]

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My channel link with your friends and help me to reach big audience

[@pinkisharma5646]

sure

[@crysteenaraniffo6112]

Please make video in indian accent

[@animatedbiologywitharpan]

This is my inherent accent. Can't really help it. Sorry. But thanks for your suggestion