Protein Sequencing - Edman Degradation

[@aircraftspotter_bareilly]

Such a life saver explanation 😊

[@EasyPeasyLearning]

Thank you ☺️

[@shreyasijati9421]

Very nice explanation mam.Thank you so much 🥰🥰

[@EasyPeasyLearning]

You are welcome 🤗

[@dmbms]

Beautiful

[@EasyPeasyLearning]

Thank you 😊

[@sivasankar372]

Explained simply. Thank you!

[@EasyPeasyLearning]

Thank you 😊

[@dimwoodland]

why can't it work if the sequence is longer than 50-70 amino acids?

[@EasyPeasyLearning]

Hi there, apology for late reply, If the sequence of Polypeptide chain is longer then 50-70 amino acids, then chances of this polypeptide to convert back in to tertiary and quaternary structures are higher even in the presence of chemicals.

[@mhamado.abdullah6925]

How many methods do we have?

[@EasyPeasyLearning]

We have Sanger method for n terminal and different methods for c terminal. Will discuss soon

[@mhamado.abdullah6925]

@@EasyPeasyLearning thanks alot

[@bollywoodonly1955]

I want to know the mechanism of the reaction.

[@EasyPeasyLearning]

Hi! You can read the details from these articles
www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/edman-degradation

[@bollywoodonly1955]

@@EasyPeasyLearning Thank you, ma'am😊.

[@EasyPeasyLearning]

You are welcome 😊

[@melkadilhara9421]

@@EasyPeasyLearning 1