Cellular reprogramming by synthetic biology (CRISPRa & CRISPRi)

Cellular reprogramming is of great interest for many reasons; studying human diseases, understanding cellular plasticity and epigenetic cell state and also for regenerative medicine such as cellular replacement or in vivo reprogramming.
Expression of Yamanaka factors was shown previously to reprogram fibroblasts cells. Expression was achieved by having exogenous expression of these genes. What if endogenous activation of these genes could be used? Well, it has been done and cellular reprogramming has been achieved by synthetic biology approaches, notably as we will discuss in this video by engineered CRISPR tools, such as CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi). These tools function as synthetic transcription factors and can be used to activate or repress gene expression, respectively.
TIMESTAMPS;
Intro - 00:00
CRISPR tools - 01:30
Cellular reprogramming - 04:45
Using tools for reprogramming - 07:10
REFERENCES;
Weltner, J., Balboa, D., Katayama, S. et al. Human pluripotent reprogramming with CRISPR activators. Nat Commun 9, 2643 (2018). doi.org/10.1038/s41467-018-05...
CRISPR-Based Chromatin Remodeling... doi.org/10.1016/j.stem.2017.1...
Review article where figures are from doi.org/10.1152/physrev.00034...
Engineering cell fate - doi.org/10.1016/j.coisb.2020....
Check out ‪@LifespanNews‬ latest video - • Can AI help design lon...
Icons in intro; "www.freepik.com/free-photos-v..."Background vector created by freepik - www.freepik.com

[@TheSheekeyScienceShow]

So here is the follow-up video...i personally think this tech is cool, whether it will extend beyond a research tool, who knows, but what do you think? :)

[@toreadordude9798]

I think it's very fascinating and I personally wish to become a cellular biologist and a neuroscientist one day for exactly this kind of reason. Hopefully one day being able to repair mental decline and health decline caused by old age.

[@jordon2074]

This tech is unbelievably cool
It feels like it’s inevitable it one days goes beyond being a research tool. The question is how long can it be until we develop a better understanding to the point where we can take it to clinic.

[@pqtfgxkuh8127]

is it possible to develope a cure for listening hair cell loss using this!!! really looking forward.

[@SeanKula]

I think altos labs will use this and I can't wait to see what they do with it.

[@Blacdragon1]

Actually very helpful. This is the only video mechanism of crispr a and i that i have found and it was well explained. Thank you!

[@lightitupcenter]

Great Job , Thanks

[@dadsheppard6248]

Amazing video- pretty complicated but great insight into the latest ideas in biology.

[@kazolthapa790]

Great content 👏🏻

[@remy7663]

the saying goes "the older you get, the more risks you're willing to take." i was wondering about the least risky things you can do for longevity more targeted for someone in their 20s, since there are so many talks about the latest drugs and procedures that are supposedly 'gamechanging'.

[@kral3046]

Intermittent Fasting, Regular Exercise, and quality sleep.

[@remy7663]

@@kral3046 Well I was thinking of the best ways to avoid the three most common causes of death: cardiovascular diseases, cancer, and pulmonary diseases. Pretty sure those three are something that starts to proliferate decades before symptoms even reveal themselves.

[@SlamminGraham]

If we could change our genes to remove damaged ones or get rid of hereditary genes which give people diseases and conditions, that would be great! Gene editing has a lot of potential applications in aging reversal.

[@ghalia2486]

I was wondering if any of these have been implicated in common neurodegenerative diseases such as Alzheimer’s or Huntington’s disease? I know Alzheimer’s is a bit more tricky since the exact cause is still up in the air, but for those who have the genetic predisposition it could be of use? Great video btw :))

[@TheSheekeyScienceShow]

so actually it seems reducing tau expression by adding methylation to the site could work (using CRISPRoff) ..but whether it can alleviate progression of AD or be therapeutically relevant is to be seen

[@rhyothemisprinceps1617]

Someone please come up with a way to reprogram plants so they don't make pollen if you don't want them to. You can experiment on the birch tree in my front yard. Maybe a chainsaw would be simpler.
Or you could figure out a way to turn off allergies - that's probably the solution we need.