FEI Tecnai F20 S/TEM: basic operation (version 2.0)
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- เผยแพร่เมื่อ 13 ต.ค. 2024
- Several months ago, myself and another faculty filmed a series of short videos regarding basic operation of our Tecnai F20 to enhance student training; I then added voice over and some interspersed images and the final product turned out nice, so I complied all of these short videos into one longer video covering basic operation of an FEI Tecnai F20 S/TEM.
I realize I already have a video covering this (which was the very first video I ever posted), so this will be the new and improved version 2.0, which is a nice improvement over the original in terms of both quality and clarity.
What's included in version 2.0:
1. How to load/unload specimens for three different types of holders
2. How to insert/retract single and double tilt holders into/from the TEM
3. How to navigate around, find the sample, and set eucentric height
4. Instrument alignment
5. Zone axis alignment
6. Recording images using a digital camera
7. How to properly finish up your session
As a little teaser, I have another basic operation video that is in the works for a new Talos F200i S/TEM we recently acquired, so that will likely be my next post.
As always, thanks for your support, and please let me know if you have any questions or comments.
Hi Nicholas, thank you so much for your videos, I really appreciate it. In addition, I was wondering if you could make some videos that are focused on how to analyze the results of TEM?
Hi Ethan, glad you like the videos; analysis of TEM results is a very broad topic that is very specific to the type of data you obtain and/or what you are trying to learn from the data; are there specific types of TEM data you are interested in analyzing?
@@NicholasRudawski Hi Nicholas, personally speaking, SAED pattern and HRTEM would be perfect. On the other hand, I guess some other people may also need this kind of tutorial since SAED and HRTEM are widely used. Last but not least, I'm open to all types of data analysis guidelines you would like to provide as you are such a good teacher.
Hi Nicholas,
is it possible to demonstrate EELS and EDS?
Thanks
Hello Nicholas, thank you for the videos. I could actually improve my microscopy skills using your videos. I guess your institute is also closed and so your videos are not coming. Meanwhile can you do a video on using TEM analysis software like JEMS, STEMcell etc? I guess many independent videos on how to do SAED analysis, multislice calculation, FFT etc. can be made.
Hi Nicholas, I have a problem with condenser lens astigmatism, I cannot make a circular beam in both directions when I am varying intensity, I can only make a round beam in one direction, I do not know what to do. I ask the other operators they told me it is Ok.
Could you please make a video about Diffraction lens Astigmatism? if the DP spots are not round is it because of Diff lens astigmatism or Focus is off?
Hi Sara; sorry to hear you are having trouble. Are you seeing the issue in image mode or diffraction mode? You are indeed correct that if the spots in diffraction pattern aren't round that the diffraction stigmators should be tweaked (unless another set of stigmators is severely misaligned). The focus in diffraction mode shouldn't really change the shape of the spots, jut the size of the spots.
@@NicholasRudawski Thank you for your reply, I see the condenser stigmatism issue in imaging mode, the beam is circular in one direction but when I change the intensity knob to the other direction it is elliptical. I have this issue in both LM and SA mode.
@@saraa2636 it actually isn't terribly uncommon to see this. To a small degree, any astigmatism in the beam shape will be somewhat dependent on the strength of the lens (in this case, the condenser). The important thing is that if you image on the "clockwise" side of crossover that the beam is circular in that regime. If it isn't circular on the other side of crossover, it really isn't of any practically concern.
N thanks
Sure thing!
Audio sketchy at first