FEI Talos F200i S/TEM: basic operation (playthrough)
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- เผยแพร่เมื่อ 4 พ.ค. 2020
- In this video, I cover basic operation (akin to a video game "playthrough") of a new FEI Talos F200i S/TEM recently acquired by the University of Florida; this encompasses all the material that is covered during basic conventional (static/fixed beam) mode training of new trainees including:
1. high-resolution (lattice) and bright field imaging
2. selected area diffraction
3. energy dispersive spectroscopy surveys
You will notice I did not include anything related to loading/unloading of specimens holders and insertion/extraction of specimen holders into/out of the column as these are identical to what is done with Tecnai instruments and I wanted to avoid redundancy.
I hope you are all staying safe! If you have any questions/comments, please let me know. - วิทยาศาสตร์และเทคโนโลยี
Nick, thank you for the amazing work! This is great material for beginners.
Hi Hanlei: you're welcome, glad you find the material helpful!
very helpful! looking forward to the high-res STEM imaging video.
why is there only a single like button on TH-cam? I cannot thank you enough for this. Best regards from Germany
Hi! Awesome video! Helped me a lot! Thanks so much! I have a question regarding the High Resolution Imaging mode, is this a phase constrast TEM?. If not, whats the main difference between this mode and normal imaging mode for a TEM of JEOL for example. Is there a substantial difference regarding resolution limit or is just branding?. Thanks in advance!
Hello Nicholas, thank you for all your effort and the nice videos. Hopefully you're having fun playing with the new microscopes!
I have two questions: In the FFT at around 36:30 one can see "extra" reflexes, which I think are due to aliasing (bigger pixelsize in view mode due to binning i suspect), i.e. "folding back" of the reflexes? Is this camera too sensitive for diffraction work or why use "only" the Flucam image?
Thank you again!
Hi TheLukmuk: I'm glad you like the videos. You are correct that the additional unexpected features in the FFT are due to the mathematics of the FFT algorithm. At some point, I should an FFT specific video, but I'm still wrapping my head around the math. As far as the camera is concerned, we simply have not been able to collect single crystal diffraction patterns with the Ceta camera without blooming of the spots. We even had an apps person from FEI here and he couldn't do it either. The Flucam doesn't suffer from this problem because it is not directly imaging the electrons, but rather is imaging the light generated from the electrons hitting a phosphor screen. Plus in diffraction conditions needing high dynamic range, it's hard to beat the high dynamic range of the phosphor screen.
Thank you for kind tutorials, which help me a lot. I'm very new about the alignment issues, which bother me a lot cause the alignment in my college is really easy to change. I have a question about that: what is moving when we move the beam track ball? What's the difference between the movements of the ball, beam shift and adjust of condensers? In alignments, in what sequence should I align? Monochronomator -> C2apeture -> ppx + ppy -> center c2 aperture -> condenser center TEM -> rotation center -> coma free align X/Y -> ppx+ppy? The beam shift seems to cover a wide operation range when I use.
Glad my videos are helping you. Regarding your questions: 1) The trackball is dedicated to shifting of the beam. You can also configure the "multifunction" knobs for "beam shift", which will then do the exact same thing as the trackball. As far as alignment order, my recommendation is: 1) set eucentric height, 2) select spot size, 3) align C2 aperture, 4) correct condenser astigmatism, 5) align beam tilt pivot points, 6) align rotation center, 7) insert/center objective aperture, 8) correct objective astigmatism. When your are describing the "beam shift" covering a wide range of operations, I think you are referring to the "multifunction" knobs (and yes, these can be configured to perform a nearly endless list of functions//alignments)
Hi Nicholas. It is possible to take SAED using ceta camera and Velox. One just needs to set capture time and viewing time to smallest available period. I guess it is 2 micro sec. and increase depending on counts. We use the ceta camera to get SAED in Themis.
Hi Rahul: even using high numerical values of spot size, small SA apertures, and short exposure times, there was still a lot of blooming in the single crystal patterns (polycrystalline samples were less of an issue); even when we had the FEI apps person here, he still couldn't produce a satisfactory pattern with the Ceta camera. We have a 10+ year old Gatan UltraScan on our older Tecnai F20 and it produces far better, blooming-free patterns, which is baffling to say the least. Plus it's hard to beat the dynamic range of a fluorescent screen in a high dynamic range application. Maybe you could make a video showing you collecting patterns on your Themis with the Ceta camera? I'd be very interested to see how you set it up and the results you obtain.
@@NicholasRudawski Surely, but we have to wait till my campus (IIT Bombay) reopens. I have few patterns taken on ceta, which I will be happy to share, please drop a mail to me. My mail id is rahul_agr@iitb.ac.in
Hi Nicholas, is it possible to demonstrate EELS mapping? Thanks
Hello! Unfortunately, not on this system as it does not have EELS; we did acquire another instrument that does have EELS but we are several weeks (if not months) away from it being operational.
looking forward to EDS&EELS mapping and analysis step by step,thank you
How can we quantify the data in EDS mode ? The last part
Hi Bijoy: it's basically a black box calculation. You input the spectrum and tell the system what X-ray peaks to use and it calculates the percentages based on theoretical sensitivity factors. This would actually be a good video, to go over how quantitative EDS works, so thanks for giving me the idea.
What is the price of this equipment?
thank you .can sent manual .how this is paper
Hi Abdullah: you're welcome; please see here:
rsc.aux.eng.ufl.edu/ccb/resource.asp?id=177
Click on the "Docs" link near the bottom of the page; then you can see and download the different standard operating procedures I have written for using this instrument. I hope this helps.