To ensure DMSO is under 1% after adding cells to suspension media. We are assuming that the freezing media is made up of 10% DMSO, 90% FBS. Identify volumes: • Let V1 be the volume of cell suspension. • Let V2 be the volume of cryovial freezing media added. Formula: • Final DMSO concentration = (0.1 x V2)/(V1 + V2) Inequality for DMSO < 1%: • 0.01 > (0.1 x V2)/(V1 + V2) Simplified condition: • V2 < (V1)/9 Add less than one-ninth the volume of the cell suspension in freezing media to keep DMSO under 1%. For example, if you have 9 mL of cell suspension, add less than 1 mL of cryovial freezing media.
How I kmow DMSO will be under 1% after adding cells? How to do the calculation?
To ensure DMSO is under 1% after adding cells to suspension media.
We are assuming that the freezing media is made up of 10% DMSO, 90% FBS.
Identify volumes:
• Let V1 be the volume of cell suspension.
• Let V2 be the volume of cryovial freezing media added.
Formula:
• Final DMSO concentration = (0.1 x V2)/(V1 + V2)
Inequality for DMSO < 1%:
• 0.01 > (0.1 x V2)/(V1 + V2)
Simplified condition:
• V2 < (V1)/9
Add less than one-ninth the volume of the cell suspension in freezing media to keep DMSO under 1%. For example, if you have 9 mL of cell suspension, add less than 1 mL of cryovial freezing media.
Is this applied to cancer cell line too?
Yes, you can use the same cell thawing procedure for cancer cell too!