mRNA Splicing

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  • เผยแพร่เมื่อ 24 ธ.ค. 2024

ความคิดเห็น • 256

  • @WellDamn.
    @WellDamn. 4 ปีที่แล้ว +196

    I cried when the intron faded away at the end

    • @dunebrowning9829
      @dunebrowning9829 3 ปีที่แล้ว +35

      Mr. ribosome I dont feel so good

    • @bandarboushi4216
      @bandarboushi4216 9 หลายเดือนก่อน

      😂

    • @fatimaalzhraanabhan9638
      @fatimaalzhraanabhan9638 8 หลายเดือนก่อน +3

      U still alive?

    • @vajia_
      @vajia_ 7 หลายเดือนก่อน +2

      You commented 4 years ago and I'm watching this video now.... Are you still crying Mr? 😢😅

    • @mehreenabdullah2655
      @mehreenabdullah2655 5 หลายเดือนก่อน +2

      I'm watching this now, and I can feel ur pain, it is indeed painful to be used by others and then get kicked off by them after being used😢😢

  • @yvikas70
    @yvikas70 16 ปีที่แล้ว +114

    I learned more from that 2 minute video, than the 100 min lecture that my professor gave.

  • @snm01
    @snm01 13 ปีที่แล้ว +168

    How many of you just went "OHHHHHH" after watching this? i know i did.

    • @potassium7794
      @potassium7794 4 ปีที่แล้ว +7

      WHOS WATCHING IN 2020 LOLMAO

    • @ilyasjamei1413
      @ilyasjamei1413 4 ปีที่แล้ว +2

      @@potassium7794 this should be a high rank animation in 2008

    • @gracewalker3300
      @gracewalker3300 4 ปีที่แล้ว +5

      do u get paid to write this

    • @riri.4393
      @riri.4393 4 ปีที่แล้ว +1

      @@gracewalker3300 idk lol
      but that's sus

    • @thakurprathvisingh4048
      @thakurprathvisingh4048 3 ปีที่แล้ว

      Mee

  • @JackWilson95
    @JackWilson95 9 ปีที่แล้ว +91

    U1 binds to 5' splice site, U2 binds to the A branch. After they've been rearranged U1 and U4 dissociate. U2 and U6 have the catalytic function with regards to the transesterification reactions.

    • @meeblings6
      @meeblings6 5 ปีที่แล้ว +1

      Thanks the way you worded it really helped

    • @thakurprathvisingh4048
      @thakurprathvisingh4048 3 ปีที่แล้ว

      What is the small droplets type thing that strikes to the various proteins in animation ??

    • @drosophilamelanogaster933
      @drosophilamelanogaster933 3 ปีที่แล้ว +3

      @@thakurprathvisingh4048 I believe it's ATP, which should be needed for the complex formation and activation

    • @thakurprathvisingh4048
      @thakurprathvisingh4048 3 ปีที่แล้ว +1

      @@drosophilamelanogaster933 thank you 😊 ☺

    • @bilguunundrakh815
      @bilguunundrakh815 2 ปีที่แล้ว +1

      it is U4 and U6 that have the catalytic function I think.

  • @ndsuvirtualcell
    @ndsuvirtualcell  15 ปีที่แล้ว +7

    Both of those terms refer to the nucleic acids (or nucleotides) that make up a strand of mRNA. The "A" refers to adenosine, and "Pyr-rich" refers to an area with a large quantity of pyrimidines. They act as signals/locations for parts of the process.

  • @ArchSpawn
    @ArchSpawn 7 ปีที่แล้ว +11

    what they dont tell you is that this is all happening at the same time of transcription... the 5 cap and the polyadenylation. 10/10 animation for beginners.

  • @hsbsbj1049
    @hsbsbj1049 2 ปีที่แล้ว +1

    "Greatest animations of all time
    were all done 12, 13, 14 years ago"

  • @LSI_MGA
    @LSI_MGA 11 ปีที่แล้ว +129

    Too bad you didn't add the names of the different parts of the spliceosome

    • @divelikejunk8557
      @divelikejunk8557 9 ปีที่แล้ว +32

      jyfdtrsextredckjhp9h
      I'm 1 year too late, haha, but here you go:
      The 5 snRNPs involved in splicing are U1, U2, U4, U5, and U6. They make up almost half the mass of the spliceosome. There are also other proteins which together with the snRPNPs make up the spliceosome.

    • @BBBuckley
      @BBBuckley 5 ปีที่แล้ว +6

      hey hows it going im from he future how is it in 2014?

    • @snehalwagh6266
      @snehalwagh6266 4 ปีที่แล้ว

      yeah appreciate

    • @tomaseduardolopezramirez2845
      @tomaseduardolopezramirez2845 4 ปีที่แล้ว

      @@divelikejunk8557 NOICE

  • @XSirApocalypseX
    @XSirApocalypseX 14 ปีที่แล้ว +6

    The 5`-End (GU) of the Intron is fixing at the Branch Point (A).
    Introns (INTervening RegiONS) are not coded Pieces of a RNA. At the Splicing, they will be depolymerised.
    Exons (EXpressed regiONS) are the coded Pieces of the RNA, at the Splicing the Exon Parts will be fixed together.
    prä-mRNA -> (Splicing) -> mature mRNA
    There are also special Introns, that go in a enzymatic-conformation after Splicing.
    Only Eukaryotes have Introns and Exons

  • @Forlo12345
    @Forlo12345 15 ปีที่แล้ว +1

    The U subunits ARE there. They make up the spliceosome. In fact, the U1 and U4 subunits are supposed to dissociate before the lariat is formed. Of course, they didn't mention that, BUT they did show the subunit corresponding to U1 dissociate from the mRNA.

  • @fatimaalzhraanabhan9638
    @fatimaalzhraanabhan9638 8 หลายเดือนก่อน +6

    Who's watching in 2024❤😢

  • @simonscholz2400
    @simonscholz2400 10 ปีที่แล้ว +24

    It's not correct right? Not all snRNP's stay with the spliceosome complex the whole proces, U1 and U4 should leave. Or am I wrong?

    • @YeahTakeItEasy
      @YeahTakeItEasy 9 ปีที่แล้ว +8

      Simon Scholz You aren't, you are right. This video is imprecise, but still helps a lot imho

  • @kasieeenda
    @kasieeenda ปีที่แล้ว +2

    this is such a wonderful video! not only did it help me immediately understand the process but also made me super amazed by it! thank you!

  • @lanamamba3459
    @lanamamba3459 7 หลายเดือนก่อน +1

    Rest in peace, intron🕊️🌹

  • @cstarvideo
    @cstarvideo 13 ปีที่แล้ว +1

    This is a great video! It would only be better if you could name the snRNPs as they attach to the mRNA. Also, one inaccuracy is that the video doesn't show U1 and U4 leaving the complex after the spliceosome is activated for the following transesterifications.

    • @ektatabiyad7759
      @ektatabiyad7759 2 ปีที่แล้ว

      Can u plz explain which sRNA bind and which form splicosome???

  • @jackiebruce9146
    @jackiebruce9146 10 หลายเดือนก่อน

    Still holds up, great quick video to show what's going on. Thanks!

  • @tctk1
    @tctk1 15 ปีที่แล้ว

    thanks........ but a A phosphodiester bond is actually formed between the 4' OH and the lactase phosphofructokinase end of the ligase ester. NT

  • @maheenhumayun3508
    @maheenhumayun3508 12 ปีที่แล้ว

    Splicing occurs in the nucleus so that then mRNA only has exons when it leaves the nucleus to go get translated and what not

  • @elysa1399
    @elysa1399 2 ปีที่แล้ว

    No way how smooth can this be omg wow

  • @Forlo12345
    @Forlo12345 15 ปีที่แล้ว

    Yeah, that is what happens. And I don't know why people are getting thumbs down for pointing that out because it is true. I will likely get thumbs down too for saying that. In order for the lariat to be formed...U1 and U2 MUST leave the spliceosome.

  • @stealthalexrifle
    @stealthalexrifle 8 ปีที่แล้ว +20

    splicesome are so kind.

  • @spicychickenwing31
    @spicychickenwing31 14 ปีที่แล้ว

    @ilikecandycane It is a process that produces alternate mature mRNA's, in which some exons are actually removed along with introns. This leads to a large diversity of gene products from a single sequence. Usually, you would have binding inhibitors or even promoters attach to an end of an exon (splicing sequence) to either inhibit or promote splicing at that end (affinity to spliceosome). Check out the regulation of alternative splicing in the determination of sex of drosophila for an example.

  • @smmount
    @smmount 12 ปีที่แล้ว

    There is a small problem here - the pre-mRNA is not cleaved. Chemically, splicing involves two phosphotransfer reactions, not cleavage followed by ligation as this video shows.

  • @MrDonMillie
    @MrDonMillie 13 ปีที่แล้ว

    @soccerchic315 alternative splicing. different exon combinations will form different proteins from the same pre-mRNA

  • @cristian1092
    @cristian1092 12 ปีที่แล้ว +1

    and what will happen with the intron later?

  • @dacatmaster83
    @dacatmaster83 12 ปีที่แล้ว

    Thanks for posting these vids! I've already watched 3 and can't stop!

  • @kittenkat88
    @kittenkat88 16 ปีที่แล้ว

    I'm sorry, what I wrote there is actually wrong. It's not removing exons, it's selection for them. Introns are the non-coding parts. Extrons code for the production of proteins. Why don't you just try the wikipedia articles on introns, exons, transcription and translation?

  • @Johnak4715anos
    @Johnak4715anos 15 ปีที่แล้ว

    It's the description that's wrong. The video is accurate.

  • @whiterose855
    @whiterose855 13 ปีที่แล้ว

    @soccerchic315 through the process of alternative splicing. a single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons.

  • @MichaelHarrisIreland
    @MichaelHarrisIreland 10 ปีที่แล้ว +35

    I'm learning this stuff for the first time, but it's so damn interesting.

  • @randomguy971000
    @randomguy971000 10 ปีที่แล้ว +2

    u r great. Would of been helpful if u included how the extron can also be furtherly spliced

  • @roarixer
    @roarixer 16 ปีที่แล้ว

    The video was good for general reference, but it fails to discuss details and the activation of spliceosome and other important proteins such as TFIIH. Also, it did not mention the importance of phosphorylation.

  • @Rajeshsanthanam
    @Rajeshsanthanam 11 ปีที่แล้ว

    The best ever (?) animation on RNA splicing

  • @roman20353
    @roman20353 7 ปีที่แล้ว +1

    I wonder how exactly they visualised this or came up with the order of work of these proteins.

  • @DBS608
    @DBS608 2 ปีที่แล้ว

    No phosphodiester bond brakes during this... just transferring of bond takes place between exons unit.

  • @hypnonebula
    @hypnonebula 15 ปีที่แล้ว

    @ChickenWingChampion I guess not proteosome, since that's for protein... Most probably, RNAse enzyme is the one degrading the introns

  • @lonelyjew
    @lonelyjew 15 ปีที่แล้ว +1

    Hmmm... This isn't what I'm learning in my genetics class. We're being tuaght that 2'OH on the A site, with the help of splisomes that change the conformation of the mRNA, attacks the phosphodiester bond at the where the first exon and intron meet making the lariet. Next, again via conformation, the exposed 3'OH of the first exon attacks the phosphodiester bond at the remaining exon/intron connection. I guess I'll just go by what we learned in class.

  • @feefriz
    @feefriz 14 ปีที่แล้ว +1

    so basically rna splicing is taking out the intron. Got it.

  • @abdalrhmandabbagh6417
    @abdalrhmandabbagh6417 5 ปีที่แล้ว

    U1 binds to 5' then u2 binds to the A branch site , then the 5' binds to the A site with help of U1 and U4 and u6 after the binding u1 and u4 leave , then u5 binds to the 3' end and cuts it , then the exons binds and that's how it's done

  • @DeemahAd
    @DeemahAd 10 ปีที่แล้ว

    cristian1092
    they said in the video that it will be degraded
    2:16
    " the introns debranched, and is then degraded"

  • @Samanimeeditor
    @Samanimeeditor 3 ปีที่แล้ว

    Thanks but I have a question that why eukaryotes bears both exons and introns and not prokaryotes ???

  • @studenthelper6042
    @studenthelper6042 ปีที่แล้ว

    why splicing of introns during transcription occurs only in eukaryotes and not in prokaryotes

  • @faithlocket
    @faithlocket 14 ปีที่แล้ว

    Is every time a little molecule "hits" a subunit an ATP hydrolysis???

  • @dm_entertainment_
    @dm_entertainment_ ปีที่แล้ว

    video posted 15 years ago and here I am with a test on Friday lol

  • @sxyngel
    @sxyngel 11 ปีที่แล้ว +2

    LOL i'm glad because im in your position now...except im nearly at the end of revision and still have few weeks left before exam :D. so boys and girls! do not start to study a day or 2 days before exam

  • @aomsxe
    @aomsxe 12 ปีที่แล้ว

    Spliceosome is composed of snRNA and other protein subunits. pre-mRNA is NOT in the spliceosome. The spliceosome works on the pre-mRNA in splicing intron.

  • @walter0bz
    @walter0bz 13 ปีที่แล้ว

    is the purpose of this DNA compression? (encoding multiple proteins) I half expect to find a version of ZLib running in molecular clockwork..

  • @MrRacquetStringer
    @MrRacquetStringer 12 ปีที่แล้ว

    This is a good video...but its not specific enough. ie) complex protein names and the interactions between each piece and the RNA.

  • @hussainzakir1
    @hussainzakir1 8 ปีที่แล้ว

    hi I was hoping you could help me, how does alternative splicing leads to a one amino acid change in protein sequence on the same gene, with all the exons being transcribed.

  • @WandsandMagic
    @WandsandMagic 15 ปีที่แล้ว

    Is this what is known as alternative splicing?

  • @likeaphoenix6
    @likeaphoenix6 3 ปีที่แล้ว +4

    I can't tell how helpful it was ✨ thanks a ton ❤️

  • @naveeeeed
    @naveeeeed 8 ปีที่แล้ว +9

    This is majestic.

  • @SpydermanGST
    @SpydermanGST 16 ปีที่แล้ว

    I don't think so. Are you thinking of the sigma factor?
    The only other thing that I can think of that you are talking about is the catalytic component of RNAP that is responsible for elongation of the transcript. That catalyzes the linkage of rNTP's with phosphodiester bond.
    If that didn't clear it up, just let me know.

  • @roarixer
    @roarixer 16 ปีที่แล้ว

    nvm...this was intended for the transcription video

  • @smmount
    @smmount 11 ปีที่แล้ว

    I stand corrected, "transesterification" is more accurate and precise.

  • @IAM469AL
    @IAM469AL 11 ปีที่แล้ว +1

    one year later, How does the furtune of your exam was ?! :)

    • @mojam6715
      @mojam6715 3 ปีที่แล้ว

      7 years later, how are you?

  • @sardineking123
    @sardineking123 11 ปีที่แล้ว +1

    how awesome... school needs to adapt and include links to videos for at home, then the classroom opens up for discussion

  • @TheReaIOptimusPrime
    @TheReaIOptimusPrime 6 ปีที่แล้ว

    good video, it would have been better if you would have had the names of the parts of the spliceosome

  • @stealthtjah
    @stealthtjah 14 ปีที่แล้ว

    My book (The Cell; Alberts) says that eucarytoic mRNA can contain noncoding sequences. I thought that mRNA is formed after removal of the introns (noncoding sequences). So does mRNA only contain coding sequences? And is it possible that 1 mRNA molecule codes for more then 1 protein? Please help me, I am a little bit confused!

  • @SeaspineEdu
    @SeaspineEdu 15 ปีที่แล้ว

    I'm looking for the 3' of the splice leader but can't identify the sequence. Any suggestions?

  • @CharlieSpencerMurray
    @CharlieSpencerMurray 11 ปีที่แล้ว

    Two transesterifications, between GU and A, and the cleaved 3' end of the exon and the AG.

  • @DDhannique
    @DDhannique 13 ปีที่แล้ว

    Is GU and AG the only bases fond at the end of introns?
    Does this mean the splice site is constant?

  • @thegto2007
    @thegto2007 15 ปีที่แล้ว

    what are
    "A" branch site
    Pyr-rich region
    ?
    or what are they doing ?

  • @conishuaa
    @conishuaa 11 ปีที่แล้ว

    inside the nucleus, only when RNA is mature it's able to get out of the nucleus

  • @soccerchic315
    @soccerchic315 13 ปีที่แล้ว

    so how does this make it possible to have 100,000 proteins with only 30,000 genes?

  • @ndsuvirtualcell
    @ndsuvirtualcell  15 ปีที่แล้ว

    Thank you for catching that--it's fixed!

  • @Johnak4715anos
    @Johnak4715anos 15 ปีที่แล้ว +1

    Your video description has an error: it says exons are removed and introns form the protein. It's the opposite.

  • @perriperripanpan
    @perriperripanpan 12 ปีที่แล้ว

    Where in the does the splicing occur? Is it outside the nucleus?

  • @ChickenWingChampion
    @ChickenWingChampion 15 ปีที่แล้ว

    how is the intron degraded? Proteosome?

  • @senior08matt
    @senior08matt 12 ปีที่แล้ว

    could be a little more detailed about the splicosome

  • @kittenkat88
    @kittenkat88 16 ปีที่แล้ว

    removing exons, the non-ciding parts.

  • @acynosure
    @acynosure 13 ปีที่แล้ว

    It'll be more useful if they point out which one is U1, U2, U4, U5, and U6

  • @MsKnaub2011
    @MsKnaub2011 12 ปีที่แล้ว

    I SOOO AGREEE WITH YOU!!!! IT'S SO EASY TO STUDY NOW WITH ALL THESE FREAKING VIDEOS.

  • @chrisjay7750
    @chrisjay7750 11 ปีที่แล้ว +1

    These videos are brilliant!

  • @343pri
    @343pri 12 ปีที่แล้ว

    hey thanx... nw i actually understood the actual process. it is so helpful.. this process i couldnt understood in todays class.. i do undestand nw....... thank u thank u so much. and plz............ do upload more such helpful video........:-)

  • @nkechi435
    @nkechi435 15 ปีที่แล้ว

    ahhh... not specific at all, what about the U proteins!

  • @Ray-jg1bw
    @Ray-jg1bw 7 ปีที่แล้ว

    Great for showing the bigger picture, thank you.

  • @obladh
    @obladh 11 ปีที่แล้ว

    you guys think that it is sad? there are (almost) no barriers between us and knowledge anymore...

  • @roninanimelover
    @roninanimelover 15 ปีที่แล้ว

    This really helped me a lot in my medical studies! Thanks! =D

  • @rubinprince100
    @rubinprince100 2 ปีที่แล้ว

    thought the intro was the lost souls beginning lmao

  • @ArtByNaNa305
    @ArtByNaNa305 14 ปีที่แล้ว

    I kinda like the weird/creepy music, lol.
    very very helpful, thanks.

  • @MrCrazyOO7
    @MrCrazyOO7 11 ปีที่แล้ว +1

    my final is tomorrow and am reading the comments, not even watching the video, "gangsta face" how sad!

  • @jasonle7522
    @jasonle7522 8 ปีที่แล้ว

    What is the purpose or function of introns?

    • @MarfoNRG
      @MarfoNRG 8 ปีที่แล้ว

      Introns, in archea and eukariotes, might contain enhancers, have meaning in the alternative splicing, might contain miRNA, they can control genetic expression (via complementarity of certain DNA regions) and they improve gene diversity.

  • @ABeardedDad
    @ABeardedDad 11 ปีที่แล้ว +1

    Pretty sure I didn't need to know that for my exam, but that was cooooooool!!

  • @fredd298
    @fredd298 12 ปีที่แล้ว

    how did it go?

  • @littlemissbossybee
    @littlemissbossybee 15 ปีที่แล้ว

    pff, the music is the best part

  • @filifjonkan94
    @filifjonkan94 11 ปีที่แล้ว

    hey, uhm I hate tro bring it up, but how did you do?

  • @francescastarkey8982
    @francescastarkey8982 10 ปีที่แล้ว

    is this bio part 1 or 2?

  • @crypticwinnow6202
    @crypticwinnow6202 6 ปีที่แล้ว

    GOOD INITIAL STEP TO UNDERSTAND SPLICING

  • @xxmztoyzxx
    @xxmztoyzxx 12 ปีที่แล้ว

    exactly there was no technical terms used whatsoever which is a bit annoying -_-

  • @Enfermagem67
    @Enfermagem67 12 ปีที่แล้ว

    ELE VAI SER APROVEITADO PELA CELULA

  • @mohsenakhavan3500
    @mohsenakhavan3500 3 ปีที่แล้ว

    Thank you very much for the nice animation. Very clear. Congratulation.

  • @grannacash
    @grannacash 11 ปีที่แล้ว

    I agree I learn more from you tube than I do @ florida College of Natural Health

  • @CharlieSpencerMurray
    @CharlieSpencerMurray 11 ปีที่แล้ว

    Both are probably more accurate than the video.

  • @harpoon67
    @harpoon67 15 ปีที่แล้ว

    Totally just said that in a Russian accent and it was amazing.

  • @Hope-qe2xr
    @Hope-qe2xr 9 หลายเดือนก่อน

    i am now in year ten learning this, i cant believe this video is older than me

  • @whateverdude789
    @whateverdude789 16 ปีที่แล้ว

    u think that's bad guys? in my country its usually about 7-8 papers in about 2 freakin months!!!! trust me having exams that drag for such a long time aint even funny!!!

  • @kobe51
    @kobe51 11 ปีที่แล้ว

    better than my so called teacher could ever do.

  • @poweredman
    @poweredman 12 ปีที่แล้ว

    Spliceosome: snRNA + pre-mRNA. This is enough for the overview i think

  • @karendelmuro8926
    @karendelmuro8926 9 ปีที่แล้ว +4

    Wonderful video, thanks. I help me in me molecular biology class