U1 binds to 5' splice site, U2 binds to the A branch. After they've been rearranged U1 and U4 dissociate. U2 and U6 have the catalytic function with regards to the transesterification reactions.
what they dont tell you is that this is all happening at the same time of transcription... the 5 cap and the polyadenylation. 10/10 animation for beginners.
Both of those terms refer to the nucleic acids (or nucleotides) that make up a strand of mRNA. The "A" refers to adenosine, and "Pyr-rich" refers to an area with a large quantity of pyrimidines. They act as signals/locations for parts of the process.
jyfdtrsextredckjhp9h I'm 1 year too late, haha, but here you go: The 5 snRNPs involved in splicing are U1, U2, U4, U5, and U6. They make up almost half the mass of the spliceosome. There are also other proteins which together with the snRPNPs make up the spliceosome.
The 5`-End (GU) of the Intron is fixing at the Branch Point (A). Introns (INTervening RegiONS) are not coded Pieces of a RNA. At the Splicing, they will be depolymerised. Exons (EXpressed regiONS) are the coded Pieces of the RNA, at the Splicing the Exon Parts will be fixed together. prä-mRNA -> (Splicing) -> mature mRNA There are also special Introns, that go in a enzymatic-conformation after Splicing. Only Eukaryotes have Introns and Exons
The U subunits ARE there. They make up the spliceosome. In fact, the U1 and U4 subunits are supposed to dissociate before the lariat is formed. Of course, they didn't mention that, BUT they did show the subunit corresponding to U1 dissociate from the mRNA.
LOL i'm glad because im in your position now...except im nearly at the end of revision and still have few weeks left before exam :D. so boys and girls! do not start to study a day or 2 days before exam
This is a great video! It would only be better if you could name the snRNPs as they attach to the mRNA. Also, one inaccuracy is that the video doesn't show U1 and U4 leaving the complex after the spliceosome is activated for the following transesterifications.
Yeah, that is what happens. And I don't know why people are getting thumbs down for pointing that out because it is true. I will likely get thumbs down too for saying that. In order for the lariat to be formed...U1 and U2 MUST leave the spliceosome.
@ilikecandycane It is a process that produces alternate mature mRNA's, in which some exons are actually removed along with introns. This leads to a large diversity of gene products from a single sequence. Usually, you would have binding inhibitors or even promoters attach to an end of an exon (splicing sequence) to either inhibit or promote splicing at that end (affinity to spliceosome). Check out the regulation of alternative splicing in the determination of sex of drosophila for an example.
U1 binds to 5' then u2 binds to the A branch site , then the 5' binds to the A site with help of U1 and U4 and u6 after the binding u1 and u4 leave , then u5 binds to the 3' end and cuts it , then the exons binds and that's how it's done
I'm sorry, what I wrote there is actually wrong. It's not removing exons, it's selection for them. Introns are the non-coding parts. Extrons code for the production of proteins. Why don't you just try the wikipedia articles on introns, exons, transcription and translation?
@soccerchic315 through the process of alternative splicing. a single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons.
Hmmm... This isn't what I'm learning in my genetics class. We're being tuaght that 2'OH on the A site, with the help of splisomes that change the conformation of the mRNA, attacks the phosphodiester bond at the where the first exon and intron meet making the lariet. Next, again via conformation, the exposed 3'OH of the first exon attacks the phosphodiester bond at the remaining exon/intron connection. I guess I'll just go by what we learned in class.
There is a small problem here - the pre-mRNA is not cleaved. Chemically, splicing involves two phosphotransfer reactions, not cleavage followed by ligation as this video shows.
The video was good for general reference, but it fails to discuss details and the activation of spliceosome and other important proteins such as TFIIH. Also, it did not mention the importance of phosphorylation.
hey thanx... nw i actually understood the actual process. it is so helpful.. this process i couldnt understood in todays class.. i do undestand nw....... thank u thank u so much. and plz............ do upload more such helpful video........:-)
Spliceosome is composed of snRNA and other protein subunits. pre-mRNA is NOT in the spliceosome. The spliceosome works on the pre-mRNA in splicing intron.
I don't think so. Are you thinking of the sigma factor? The only other thing that I can think of that you are talking about is the catalytic component of RNAP that is responsible for elongation of the transcript. That catalyzes the linkage of rNTP's with phosphodiester bond. If that didn't clear it up, just let me know.
My book (The Cell; Alberts) says that eucarytoic mRNA can contain noncoding sequences. I thought that mRNA is formed after removal of the introns (noncoding sequences). So does mRNA only contain coding sequences? And is it possible that 1 mRNA molecule codes for more then 1 protein? Please help me, I am a little bit confused!
hi I was hoping you could help me, how does alternative splicing leads to a one amino acid change in protein sequence on the same gene, with all the exons being transcribed.
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I cried when the intron faded away at the end
Mr. ribosome I dont feel so good
U still alive?
You commented 4 years ago and I'm watching this video now.... Are you still crying Mr? 😢😅
I'm watching this now, and I can feel ur pain, it is indeed painful to be used by others and then get kicked off by them after being used😢😢
I learned more from that 2 minute video, than the 100 min lecture that my professor gave.
Haha
hey u still alive?
I want to ask the same Q😭😭 @@noblevenom2858
How many of you just went "OHHHHHH" after watching this? i know i did.
WHOS WATCHING IN 2020 LOLMAO
@@potassium7794 this should be a high rank animation in 2008
do u get paid to write this
@@gracewalker3300 idk lol
but that's sus
Mee
U1 binds to 5' splice site, U2 binds to the A branch. After they've been rearranged U1 and U4 dissociate. U2 and U6 have the catalytic function with regards to the transesterification reactions.
Thanks the way you worded it really helped
What is the small droplets type thing that strikes to the various proteins in animation ??
@@thakurprathvisingh4048 I believe it's ATP, which should be needed for the complex formation and activation
@@drosophilamelanogaster933 thank you 😊 ☺
it is U4 and U6 that have the catalytic function I think.
what they dont tell you is that this is all happening at the same time of transcription... the 5 cap and the polyadenylation. 10/10 animation for beginners.
Both of those terms refer to the nucleic acids (or nucleotides) that make up a strand of mRNA. The "A" refers to adenosine, and "Pyr-rich" refers to an area with a large quantity of pyrimidines. They act as signals/locations for parts of the process.
Too bad you didn't add the names of the different parts of the spliceosome
jyfdtrsextredckjhp9h
I'm 1 year too late, haha, but here you go:
The 5 snRNPs involved in splicing are U1, U2, U4, U5, and U6. They make up almost half the mass of the spliceosome. There are also other proteins which together with the snRPNPs make up the spliceosome.
hey hows it going im from he future how is it in 2014?
yeah appreciate
@@divelikejunk8557 NOICE
I'm learning this stuff for the first time, but it's so damn interesting.
same bro so coooool
splicesome are so kind.
The 5`-End (GU) of the Intron is fixing at the Branch Point (A).
Introns (INTervening RegiONS) are not coded Pieces of a RNA. At the Splicing, they will be depolymerised.
Exons (EXpressed regiONS) are the coded Pieces of the RNA, at the Splicing the Exon Parts will be fixed together.
prä-mRNA -> (Splicing) -> mature mRNA
There are also special Introns, that go in a enzymatic-conformation after Splicing.
Only Eukaryotes have Introns and Exons
this is such a wonderful video! not only did it help me immediately understand the process but also made me super amazed by it! thank you!
"Greatest animations of all time
were all done 12, 13, 14 years ago"
It's not correct right? Not all snRNP's stay with the spliceosome complex the whole proces, U1 and U4 should leave. Or am I wrong?
Simon Scholz You aren't, you are right. This video is imprecise, but still helps a lot imho
The U subunits ARE there. They make up the spliceosome. In fact, the U1 and U4 subunits are supposed to dissociate before the lariat is formed. Of course, they didn't mention that, BUT they did show the subunit corresponding to U1 dissociate from the mRNA.
Rest in peace, intron🕊️🌹
I can't tell how helpful it was ✨ thanks a ton ❤️
Still holds up, great quick video to show what's going on. Thanks!
This is majestic.
It's god man
This video is very helpful. I love biochemistry! Woohooo!
No way how smooth can this be omg wow
Thanks for posting these vids! I've already watched 3 and can't stop!
The best ever (?) animation on RNA splicing
LOL i'm glad because im in your position now...except im nearly at the end of revision and still have few weeks left before exam :D. so boys and girls! do not start to study a day or 2 days before exam
This is a great video! It would only be better if you could name the snRNPs as they attach to the mRNA. Also, one inaccuracy is that the video doesn't show U1 and U4 leaving the complex after the spliceosome is activated for the following transesterifications.
Can u plz explain which sRNA bind and which form splicosome???
It's the description that's wrong. The video is accurate.
how awesome... school needs to adapt and include links to videos for at home, then the classroom opens up for discussion
These videos are brilliant!
Yeah, that is what happens. And I don't know why people are getting thumbs down for pointing that out because it is true. I will likely get thumbs down too for saying that. In order for the lariat to be formed...U1 and U2 MUST leave the spliceosome.
this was so helpful, thank you very much
Wonderful video, thanks. I help me in me molecular biology class
u r great. Would of been helpful if u included how the extron can also be furtherly spliced
Great for showing the bigger picture, thank you.
@ilikecandycane It is a process that produces alternate mature mRNA's, in which some exons are actually removed along with introns. This leads to a large diversity of gene products from a single sequence. Usually, you would have binding inhibitors or even promoters attach to an end of an exon (splicing sequence) to either inhibit or promote splicing at that end (affinity to spliceosome). Check out the regulation of alternative splicing in the determination of sex of drosophila for an example.
thanks........ but a A phosphodiester bond is actually formed between the 4' OH and the lactase phosphofructokinase end of the ligase ester. NT
and what will happen with the intron later?
@soccerchic315 alternative splicing. different exon combinations will form different proteins from the same pre-mRNA
Pretty sure I didn't need to know that for my exam, but that was cooooooool!!
U1 binds to 5' then u2 binds to the A branch site , then the 5' binds to the A site with help of U1 and U4 and u6 after the binding u1 and u4 leave , then u5 binds to the 3' end and cuts it , then the exons binds and that's how it's done
I'm sorry, what I wrote there is actually wrong. It's not removing exons, it's selection for them. Introns are the non-coding parts. Extrons code for the production of proteins. Why don't you just try the wikipedia articles on introns, exons, transcription and translation?
Thank you very much for the nice animation. Very clear. Congratulation.
@soccerchic315 through the process of alternative splicing. a single primary transcript can be spliced into different mRNAs by the inclusion of different sets of exons.
No phosphodiester bond brakes during this... just transferring of bond takes place between exons unit.
Thank you for catching that--it's fixed!
Hmmm... This isn't what I'm learning in my genetics class. We're being tuaght that 2'OH on the A site, with the help of splisomes that change the conformation of the mRNA, attacks the phosphodiester bond at the where the first exon and intron meet making the lariet. Next, again via conformation, the exposed 3'OH of the first exon attacks the phosphodiester bond at the remaining exon/intron connection. I guess I'll just go by what we learned in class.
There is a small problem here - the pre-mRNA is not cleaved. Chemically, splicing involves two phosphotransfer reactions, not cleavage followed by ligation as this video shows.
I SOOO AGREEE WITH YOU!!!! IT'S SO EASY TO STUDY NOW WITH ALL THESE FREAKING VIDEOS.
Splicing occurs in the nucleus so that then mRNA only has exons when it leaves the nucleus to go get translated and what not
@ChickenWingChampion I guess not proteosome, since that's for protein... Most probably, RNAse enzyme is the one degrading the introns
The video was good for general reference, but it fails to discuss details and the activation of spliceosome and other important proteins such as TFIIH. Also, it did not mention the importance of phosphorylation.
I kinda like the weird/creepy music, lol.
very very helpful, thanks.
This is beautiful thanks so much
This really helped me a lot in my medical studies! Thanks! =D
Where are you now😢😢
video posted 15 years ago and here I am with a test on Friday lol
This video is very useful. Thanks a lot
I agree I learn more from you tube than I do @ florida College of Natural Health
hey thanx... nw i actually understood the actual process. it is so helpful.. this process i couldnt understood in todays class.. i do undestand nw....... thank u thank u so much. and plz............ do upload more such helpful video........:-)
nvm...this was intended for the transcription video
I stand corrected, "transesterification" is more accurate and precise.
Spliceosome is composed of snRNA and other protein subunits. pre-mRNA is NOT in the spliceosome. The spliceosome works on the pre-mRNA in splicing intron.
pff, the music is the best part
GOOD INITIAL STEP TO UNDERSTAND SPLICING
Two transesterifications, between GU and A, and the cleaved 3' end of the exon and the AG.
This is a good video...but its not specific enough. ie) complex protein names and the interactions between each piece and the RNA.
so basically rna splicing is taking out the intron. Got it.
I wonder how exactly they visualised this or came up with the order of work of these proteins.
Great question
Thank you :)
What a great video!
just what i needed, thanx!!
is it a bird? is it a plane? NO! its DentistMan
Simply Brilliant
cristian1092
they said in the video that it will be degraded
2:16
" the introns debranched, and is then degraded"
Totally just said that in a Russian accent and it was amazing.
ELE VAI SER APROVEITADO PELA CELULA
this video helpd a lot......
is the purpose of this DNA compression? (encoding multiple proteins) I half expect to find a version of ZLib running in molecular clockwork..
you guys think that it is sad? there are (almost) no barriers between us and knowledge anymore...
inside the nucleus, only when RNA is mature it's able to get out of the nucleus
It'll be more useful if they point out which one is U1, U2, U4, U5, and U6
I don't think so. Are you thinking of the sigma factor?
The only other thing that I can think of that you are talking about is the catalytic component of RNAP that is responsible for elongation of the transcript. That catalyzes the linkage of rNTP's with phosphodiester bond.
If that didn't clear it up, just let me know.
why splicing of introns during transcription occurs only in eukaryotes and not in prokaryotes
Fascinating
that is so beautiful.
amazing!!
Just amazing!!
removing exons, the non-ciding parts.
better than my so called teacher could ever do.
i am now in year ten learning this, i cant believe this video is older than me
my final is tomorrow and am reading the comments, not even watching the video, "gangsta face" how sad!
My book (The Cell; Alberts) says that eucarytoic mRNA can contain noncoding sequences. I thought that mRNA is formed after removal of the introns (noncoding sequences). So does mRNA only contain coding sequences? And is it possible that 1 mRNA molecule codes for more then 1 protein? Please help me, I am a little bit confused!
i cried
that was beautiful *snif*
Is every time a little molecule "hits" a subunit an ATP hydrolysis???
Is this what is known as alternative splicing?
hi I was hoping you could help me, how does alternative splicing leads to a one amino acid change in protein sequence on the same gene, with all the exons being transcribed.
Both are probably more accurate than the video.
Thanks but I have a question that why eukaryotes bears both exons and introns and not prokaryotes ???
Lovely!
its very clear thanks a lot
Where in the does the splicing occur? Is it outside the nucleus?
one year later, How does the furtune of your exam was ?! :)
7 years later, how are you?
Your video description has an error: it says exons are removed and introns form the protein. It's the opposite.
yes, I've noticed too
Una figata assurda :O