Don't miss the exciting next episode of connecting neurons to a computer here: th-cam.com/video/c-pWliufu6U/w-d-xo.html We tested to see if neurons can grow in DMEM to see if our home made recipes stand a chance of working.
I'd love to see you start a project genetically engineer a mango tree to survive anywhere and it's fruit provide 100% human nutritional requirements.... Even if it's your cyborg future self that succeeds it would be a wonderful contribution to humanity 🙏
I doubt you will see this comment, but I do think you have even cheaper options for the drinks. Using powdered gatorade and buying it in bulk not only gives you the ability to customise your solution, but also makes things even cheaper, 1/2 to 1/4 of the price in fact. But then there are other alternatives you could test that are even cheaper, because this is the cheapest you get for HUMAN consumption, but horse electrolytes for example are a thing, and they can be as cheap as 1/2 of the price of powdered gatorade. So the cheapest possible? You could get down to a nice 8x cheaper than what you got in this video, and if you are able to make a deal wil a company for really large orders, you could go down as much as 10x cheaper, we are talking less than 50 cents per litre here. In fact, horse electrolytes when bought in big bulks, like hundreds of gallons, can be as cheap as 10 cents per litre! Ridiculous.
A quick tip, If you want JAPANESE drinks such as Pocari, you can ship from the Philippines Instead (where i live), It Is SUPER cheap here and I’m sure the shipping Isn’t as expensive either! Cheers!
I work for a college and one of my jobs is to order supplies for medical labs. The reason for ordering expensive supplies is because we are state funded and to remain compliant, everything has to have a justification. If we were ordering tons of Gatorade instead of DMEM it would be hard to justify that a sports drink is being used for a lab setting. Additionally, there is oversight bodies that come around to ensure things are being conducted properly, so if a lab has a bunch of Gatorade laying around where there isn't supposed to be food and drink the school can get penalized and fined. Also, many departments receive annual budgets that are adjusted based on usage, so if one department does not use their full budget, then their next yearly budget gets reduced and any remaining funds are taken away and redistributed across the organization. So, departments tend to buy premium supplies and equipment to use up their budget in hopes to have a larger budget the following year, or at least, maximize the usage of their budget.
One important thing to keep in mind, these cell lines were started and maintained in DMEM with FBS. This means that cells that preferred DMEM and FBS thrived, while those that didn't died off. With rapidly multiplying cells, it would likely be quite feasible to gradually wean the cells entirely off of DMEM and FBS and onto a substitute. This may be much easier than trying to generate cocktails of growth factors. Another key point is that the cells themselves often make the hormones they want. This is why many cell lines prefer partial media changes rather than complete media changes. This way, the cells always get exposed to most of the hormones they've generated.
@@jacobwiens659 For sure. The cost involved in obtaining the growth media are one of the biggest obstacles to bringing down the cost of production. (The cost of electricity is another, but we already have a lot of information on how to solve that)
@@skunkjobb It's _LITERALLY_ thinning out a product. Did you not catch that they are using the Gatorade and other drinks as partial replacement for the cell media? Or all that various other stuff as partial or complete replacement for the serum?
It's crazy to think that common grocery store items could potentially replace some of the most expensive elements in the lab. Very eager to see where this leads to, and how it could potentially revolutionize lab grown meat.
Not entirely. There's almost always going to be minor or major compromises. You might be able to filter or separate out some of the undesirable elements, but every bit of that effort eats into the cost/time savings of using the retail alternative in the first place. But if something along that cheaper price point spectrum works "good enough" with 99.8% or whatever effective substitution, then yeah. For example, Thought Emporium mentioned two differences: the sports drinks significantly dropped pH balance out of the optimal cell survival range, and some trace elements in DMEM are situationally important for stuff like growing bones. I'm no cell biologist, but I'd imagine there are more nuances like these that they didn't mention. Another big one is quality control and precision to prevent variation. Two units of the same sports drink from different bottling plants or batches in the same plant could have a huge 0.2% variation in concentration of some elements. Not enough for a consumer to taste more or less salty, but could be enough to throw a precise lab process out of whack. A lot of the extra $100s of cost is for processes that ensure these error margins are orders of magnitude smaller. So the discount sports drink doping might be good enough for growing boneless McRibs or chicken nugget paste, but not for pseudo pork chops or beef ribeyes that need more realism. Good budget tier alternative for producing off-brand or generic synthetic meat, while the top quality synthetic meat will cost 2X more just to add the necessary 0.2% trace elements or whatever. Just like current consumer product price tiers. Or the discount stuff could work great for pumping out lower cost protein feedstock to feed to actual animal livestock that's raised for real meat. Not as environmentally or cost optimal as directly growing a high quality vat steak, but maybe it would be an interim technology that cuts cost inputs and greenhouse gas emissions by 10% or something, per pound of real beef produced. That's basically what industrial agriculture has been doing at a lower tech level for the past century -- grinding up undesired wild caught fish bycatch and waste parts from farmed fish to make fish meal, which they then mix into feedstock for more farmed fish or other livestock. Same for waste parts from butchered cows, pigs, chickens, etc that are fed back into the system to feed new animals. (and yes, this has been a source of increased system vulnerability to contamination, disease, and things like growth hormones recirculating back into the system as it approaches both higher efficiency and a slightly more closed loop of production)
The reasoning is because those expensive mediums are specifically tested and the ratio of contents confirmed to not have anything additional in there to not throw off experiments. It’s the same reason anything “aircraft grade” is expensive. It’s also the same reason NIST sells peanut butter for like $400. You’re not paying for the item but for the paper trail to validate your results. This is especially important in healthcare research in the case of any kind of side effects or other issues later on. Stuff like this is definitely great to know, especially if you’re just doing some preliminary research into a topic, but if you’re publishing a paper then you should be using standardised ingredients/processes.
Wouldn't be the first time something like that happened. A while back a lab tested using the old Shrinky Dinks toy to create patterns for use in microfluidics. It worked.
Pocari sweat can be found at most Asian markets both in the US and Canada, with many online retailers also shipping it from local warehouses. No real need to have it imported. Also it comes in its original powder form if you're worried about shipping weights. But all in all its effectively a Japanese clone of Gatorade made by Otsuka Pharma.
Also yes I wanted to mention they come in power form. I remember being in police cadet corps and our outfield ration included the powder. We mixed it into our water bottles that were brewing in the hot sun, and chugged it - horrible but delicious. Also had to make lunch using some cheap off-brand instant noodles, and I think some folks forgot to pour out water into the mestin for the noodles before mixing the PS powder. But anyways we opened a tin of sardines in tomato and threw that into the mestin of noodles and honestly, pretty good!
I wondered why he imported it when I can get a bottle of pocari from a local asian store in London for a few pounds. I'd be surprised if you couldn't also in Canada
@@theendoftheworldhasbeenqui2485 honestly it was probably just because its the best way to make sure you get the exact same product. yes its almost guaranteed to be the same locally but it might not be. Also i wonder if the powder might not be better because you could adjust the concentration if need be
Since coconut water performed relatifly good as a FBS replacement, maybe try turning other sorts of nuts/seeds into some sort of seed juice. Combining animal and plant produce could also be a way to broaden the nutrient composition, like mixing eggs and coconut water.
@@darthplagueis13I can’t imagine being satisfied with the sweetness of a Gatorade, tbf. they’d be majority spice and chemicals which is something but isn’t the ratio to be power puff girly enough (according to my own conjecture let’s be real for a sec lmfao-)
For processing the eggs, take a look at what was used for “albumen print” photographic processes. The literature is not the easiest to find, it’s mostly in century old photographic journals. The goal was to have a clean and optically clear protein gel into which silver nitrate grains would be suspended.
A few friends in my department (USU Biological Engineering) are working on creating an FBS substitute using subcritical water hydrolysis. They take algae and put it under high pressure and temperature to break down the algae into metabolites cells can use. The goal was to produce a vegan media that Upside Foods could use for cultured chicken. Pretty neat!
Using Gatorade of all things to grow meat, just amazing. It just feels like an april fools joke or onion article "Mad scientist makes breakthrough cost reduction in artificial meat production, Sponsored by Gatorade".
I'm gonna guess "as soon as we figure out how to make it actually work" In fact, i would assume there's already at least some footage recorded of testing and such
If this channel has taught me anything it's that biochemists absolutely fkn love E.coli and use it a lot more than the average Joe not in the know would ever expect
Yep from what I've seen is since they reproduce so fast, they can quickly see any effects, changes & then go from there.. But I'm no certified scientist
@@am529 I know I'm more so just commenting on how E.coli is used far more in biochemistry than anyone not in the know would ever expect. I for one didn't know until I stumbled on this channel just how much it's used in this field of work.
They're just overall pretty chill dudes. Very relaxed growth requirements, readily uptake DNA if zapped and/or sauna-ed a bit, don't stink to high heaven, they even come in nonopthogenic!
About 25 years ago when I was a student, I was chatting to a biochemist about food and they mentioned that they'd never eat a custard-filled doughnut, because egg custard is a great growth medium and she'd seen how well it works for bacterial cultures.
So who's the winner? Myself, who has got to enjoy custard filled donuts many times with out negative consequences? Or the person that cut themselves off from experience that cos fear of bacteria
The comment count is already nearing a thousand so I don't know if anyone reads this, but wouldn't it have been more beneficial to use carbon dioxide or some acid that you could later precipitate out, to separate the whey from milk? Vinegar (mostly acetic acid) is a weak acid with small molecular sizes that can pass cell membranes, so even after neutralizing the pH, it would keep reacting inside and outside the cells and mess up things. At least that's why one should never adjust aquarium water's pH with weak acids. Doing so could easily end up killing the fishes. Only use strong acids (nitric, sulfuric, muriatic) for that, which leave chemically more inert acid residues. Not sure if any of the aforementioned ones would be suitable for this purpose though. That's why I suggested either carbon dioxide (at least it's the easiest to obtain and apply with sodastream, not sure if there are other acid forming gasses that have high vapor pressure) that could be mostly evaporated from whey by bubbling the solution with nitrogen gas (pumping just air would remove the CO2 as well, but it might also oxidize something important), or maybe there's some suitable acid that would precipitate during the neutralizing process, like oxalic acid + calcium hydroxide. Just the first few ideas that came to my mind.
@@Suriel-e5g You'd get lots of foam, and coagulated proteins separating from whey. More importantly, I'm looking forward for your taste report, which is the, erm.. more interesting part 🙃
@@NoAIStudios we re about 50 years away from every home having their own meat synthesizer in the basement that you just need to fill the vats with gatorade and eggs every month
I'm happy that you looked into this as most organisms aren't too picky about what they ingest to survive and most of what we consume is either organic or derived from something that is. Like you said in the video: the biotech supply industry intentionally runs up the prices of otherwise inexpensive compounds simply because they know they can due to the grant money flowing into universities and other institutions. Without people working to innovate outside the current system: very little progress will ever be made on things that major corporations aren't interested in. Just the same as how the home computer hobbyists brought about the innovations needed for a truly digital age: biotech enthusiasts will need to learn how to progress outside of the (mostly closed-off) mainstream in order to make real progress in things.
Yup, very often in the economy the most profitable solutions aren't the most innovative ones but rather the most stagnant ones. No need to waste money developing a new solution (in multiple meanings of the word) if you can just keep forcing people to buy the current one.
8:49 I'm pretty sure the lab supplier sells them like that for consistency across experiments for being able to replicate experiments from papers. It's like how the ISO standard tea is like 500 dollars for a very bad and bland tea.
Not sure who came up with removing all variations in order to 'baseline' ; it's a monocultural bland paradigm and actually eliminates any spice which extends life and expands consciousness. We need to understand and implement the interaction between rosemary, cell growth and antibacterial properties for instance as this among others can play a vital part in organic growth overall
@@jonhinman2471 Removing variation is the only way to be sure your results are the the product of the experiment and not some outside force. I've never seen anyone argue against that. Then in the experiment you can make as many/much variations as you want.
Idiots click on adds and generate revenue. TH-cam has no interesst in actual humans. That's why channels aimed at children make 100x more money then channels aimed at adults. Adults have ad- and/or sponsorblocks and mostly don't click on ads. Kids do.
I'm a little confused - wouldn't the most important test here be a control with sterile water diluting the DMEM? To see if the alternatives are either really helping (like DMEM) or just not hurting anything while enough DMEM is available. Sorry if it's a dumb question.
Nah that's a good point. If DMEM can be diluted with distilled water and still work well, that's an instant drop in costs cuz every lab has access to distilled water. Heck, AC units make it as a side effect of cooling the air
Could be, but the 100% version with replaced fbs does remediate the results somewhat, Although yeah the data is better with dmem 0-100% with reverse osmosis distilled water dilution, it's not that complicated to do too, However the research from japan in video is probably more complete,
If my bio knowledge hasn't failed me, I think that's a negative control: a group that doesn't receive treatment and isn't expected to produce a result.
Great video, but I also wanted to add some information about university research, for anyone interested 1. When getting grants Universities first take a portion for the use of facilities, this can go up to 50% but I believe 20-30% is standard 2. Assuming your lab isn't big enough for some major lab equipment like an NMR the university often has a communal one...that you must pay to use 3. The lab also needs to pay for graduate student tuition and wages 4. Now that the majority of the money has probably disappeared you now have to deal with whatever miscellaneous costs appear, maintenance of equipment, purchasing chemicals, traveling to scientific conventions Academia is basically a black hole that devours money
Such a shame. But it is the world we live in, and money seems to be the best tool we could've come up with. It's both one of my favorite and most hated inventions, haha.
Yeah it can be a pain, my advisor basically doesn't have free time with the constant grant applications he has to file, granted it is a fairly large lab
@@ritishifyThis is not an issue of money itself, but of equitable relations. Universities consistently choose to exploit their facility, staff, and students because they can and are incentivized to do so.
@@jinmushui1soul I see. Although I still think that more money would probably radically change things, I guess the issue is more about the management side of things as well... I hope the situation gets better soon
@Dylan-ln6qt 1. When getting grants Universities first take a portion for the use of facilities, this can go up to 50% but I believe 20-30% is standard HAHAHA, it's over 60 at R1s. I think Duke is ~60, MIT is around ~65, my university is also around 60-70.
Holy shit the concept is freaking genius If this works on large scale And is refined The cost of animal tissue culture will reduce by a significant amount
@@chriswheeler6092 raising and killing a cow is a lot more immoral than growing meat. One requires death and suffering the other doesn't, and no I'm not a vegan.
Now we need the reverse study: how much Gatorade can you replace with dmem before gamers stop seeing advantages Will dmem become the sports drink of top athletes?
Guys are really about to put out an open-source generic brand DMEM. I'm interested now how Aquarius, another Japanese Pocari Sweat competitor, would do. There's also apparently now a "new" Green Dakara, would be interesting to see if it does any different than the regular Green Dakara. I can help answer part of why the nutritional yeast did so well: it's literally just dead cells. When we brew, we use yeast nutrient, which is honestly just mostly dead yeast. In fact, a quick and dirty way to make yeast nutrient is to just boil a packet of bread yeast for a few minutes. The dead cells contain a lot of the necessary building blocks for new cells to grow, for obvious reasons. It's really crazy to me that any lab can just casually say they can order some DNA to get printed. Imagine telling someone that 100 years ago.
Yeah but that kinda spooks me about bioterrorism, realistically while it may not be full on Tom Clancy's The Division tier, the idea that someone could just begin making designer viruses has got to be about one of the most terrifying concepts in the history of humanity also.
With regards to using milk, about a hundred+ years ago physicians did try using milk as a blood replacement for transfusions. It didn't work, but I thought it was interesting to consider that other people throughout history were on a similar path.
Not everyone has a local Asian market. I know my town sure doesn’t, and it’s the second biggest in the state I’m in. The most Asian thing I can buy here is like… lo mein noodles from an actual Asian restaurant and offbrand pockys from Trader Joe’s
Thing is, Thought Emporium is a Canadian channel, and outside of major cities it can be very difficult to find ethnic markets that sell stuff like that. Even within the shops that do, it can be difficult. I know here in Manitoba, there's a store in Winnipeg called Oomomo, it specialises in Japanese products. I have not once seen Pocari Sweat or Dakari Green there, but even if i had the stock changes with whats popular in Japan. but Japanese products in general can be hard to locate here. Most Asian markets i've seen cater more towards Chinese and Filipino products as we just have a much larger population of Chinese and Filipino immigrants than we do Japanese.
@@solofdragons6446inside London, Ontario, there are a couple of Asian Markets, and I'm decently sure they have those there, if he lives nearby or wants to make the trip, it's a good idea to check.
its so cool being able to watch these videos now having done cell culture in lab. this channel was such a huge inspiration for me over the last six or so years
This was a fascinating video. Although not currently studying in the field of biology, I am curious to know if a symbiotic culture like Kefir could make milk, or similar products, better growth media. Anyways, great video as always. Thank you for making such content free, it's been a very long time since I've been to a genetics lab as a student and I miss those days. Good luck with every project.
I was lucky enough to work in a chemical plant as an operator, yhe company was the type you'd take a concept to, and they'd do the R, D & P... and never before in my life have I been more regretful than then... the fac I didn't study chemistry GUTS me.. or any the sciences tbh. Literally EVERYTHING is chemistry. And it blows my damn mind.
@@StoneBox_761a He used to have perfect grammar, but then he drank some heads. That's why he's regretful he didn't study chemistry in middle school. Quite a sad story.
Fellow biologist here, I think this should be someone's master's thesis or student project and it is showing promise to be a startup one point. A big however, the only reason I believe that DMEM's can't be replaced by homemade recipes in academia is not the growth issue, more about replicability of the experiments. It's already so hard to follow protocols even though you use the exact brand, the exact amount indicated in the paper. Other than this, let's break the monopoly of lab suppliers lol.
@@kokofan50 as the video says, more or less each DMEM contain the same ingredients however such small differences may create certain issues. The point here is the homemade recipes will complicate the issue even more and academia will probably won't like it.
Hell yeah, let's break those price gouging monopolies and cartels that make research unnecessarily expensive!!! Also, I agree small differences in cell culture media do cause issues. But in a hypothetical situation where labs are making their own DMEM from the same recipe, I believe from my experiences in cell culture that variability that already exists due to differences between lots of FBS is way more significant than the likely differences between homemade batches of DMEM substitute. I hope that sentence made sense.
I’m actually doing a project on this! I’m a 11th grader in Germany, and for my finals, I’m doing a presentation testing accessible methods for growing tissue cultures. It’s part of our final-year research component, where we’re encouraged to choose topics with scientific or social relevance. This video was what inspired me!
For a moment there I thought those cartons said "Concentrated concentrate" I was thinking "That must be some INCREDIBLY concentrated...whatever the fuck it is!" 🤦😅😑 I need sleep, but AFTER this awesome vid!
Man, you're freaking awesome! You make biotech so easy! These videos are awesome! But I do miss those older videos where you'd teach us making diy mods or even a whole new piece of equipment, like the nanodrop style spectrometer!
bruh 13:20 was such a genius prediction from whoever edited or scripted the video! i literally zoned out and got caught spot on, i enjoyed that !! thats a whole different layer of humor
I think testing electrolyte powders would be an interesting next step, there are unflavoured ones that can be purchased in bulk for very cheap( $20 for 250L). Would remove any additives from the equation.
At least 2-3 states have, or are about to, ban the sale of cultured meat products. So what we need is a simple, affordable "kit" to grow meat at home. Something akin to gardening, mushroom cultivation, or home brewing. I feel like we're watching the development of such a kit with every new video here! I would love a simple kit/machine where all I'd need to do is subscribe to some kind of service for regular deliveries of raw materials, nutrients, etc., plop those into a machine like an inkjet cartridge, and grow cheap, infinite steaks at home. Also, kindof shamefully curious to sample my own cells and grow some my own "long-pork-belly." Honestly, I bet I'd be delicious...
I don't think that will ever fly because it's not just the "affordable kit" that's involved in this. It's also all the equipment required and the sterilization and handling protocols required to grow meat tissue in labs. This team is trained in that area, and even then they're prone to mistakes because that's how it goes. So letting the average Joe meddle with this sounds like a good recipe for an outburst of salmonella or something like that. I'm not a chemist or a biologist, but with my experience in IT and programming I can attest: the hardest thing to do is making your process user-proof. Someone *will* find a way to screw things over, and in the case of foods, the responsibility falls back on the distribution company.
@@sparking023 Off the top of my head, I'd ask if you knew anyone who ever sued hasbro for their kid getting food poisoning from an Easy-Bake Oven cake. Or, alternatively, sued betty crocker after using unrelated salmonella-infected eggs in a cupcake batter and let their kid lick the spoon. It seems to me that whatever products would be made of a kit like this would fall under the same kind of legal classifications. As long as the obvious, instructed, and prescribed usage of the kit and its supplementary products is safe, user mishandling would be seen as exactly that, and not tie back to the kit distributor.
@@sparking023tbf (a small amount of) people (non-commercially) can their own food which also requires massive precaution in handling It wouldn’t be in supermarkets but I could see a world where it becomes a culinary niche as opposed to a home lab niche
Your channel is the most interesting thing I've found on TH-cam ever. I wish you success so you can keep working on the projects you want to do and for us to watch them
I didn't realize you from Canada, as a fellow Canadian I recommend checking out asian grocery stores, especially chains like T & T as they are likely to carry Pocari Sweat and Dakara. They don't always have it available, as it tends to be a more seasonal import during the summer, but they do carry it!
18:32 Try combining the top performers to test if they can complement each other somehow. Maybe a mixture of some can have a more complete nutrient profile for the cells. Also another idea to try: "Soylent complete meal powder". It's supposed to be a balanced mixture of most nutrients the body needs.
God, i love science. >laberatory technicians developing a nutritional aid for artificial cell growth in labs< _"Wait... What if we just swap it out with Japanese Gatorade?"_ *>actually works
I would have loved to see a control with water or saline used to dilute the mixtures (even though that would probably lead to death pretty fast, but it would kinda put into perspective how beneficial the gatorade ect were)
A part of me wonders if you can take the best preforming things and basically use it as a recipe for cell production. Theres also a good video by Nighthawkinlight about an experimental procedure to change a lot of variables at once and analyze the results without having to change one factor at a time and doing a ton more work. Doing something like that as a followup could be interesting. Also keep in mind that doing this at home means that you don't need perfect results just a "good enough" standard and I'm sure this can do that. Also maybe in the future you can look at doing it entirely from scratch like you did with the soup.
Dude, you guys are so fucking awesome. I'm guessing you aren't alone working on these which is why I say you guys, so correct me if I'm wrong. I love this shit.
that was some really cool results. I noticed the best fbs 100% replacements were not also the best at 50%. I'd be curious to see the fbs test with 75% added for an extra data point
![ลองสุ่มไอโฟน 990 โกงมั้ย? [ โกงมั้ยครับ ep.101 ] | DOM](http://i.ytimg.com/vi/bfsWe0j492k/mqdefault.jpg)
Don't miss the exciting next episode of connecting neurons to a computer here: th-cam.com/video/c-pWliufu6U/w-d-xo.html
We tested to see if neurons can grow in DMEM to see if our home made recipes stand a chance of working.
Yuh
I'd love to see you start a project genetically engineer a mango tree to survive anywhere and it's fruit provide 100% human nutritional requirements.... Even if it's your cyborg future self that succeeds it would be a wonderful contribution to humanity 🙏
I doubt you will see this comment, but I do think you have even cheaper options for the drinks. Using powdered gatorade and buying it in bulk not only gives you the ability to customise your solution, but also makes things even cheaper, 1/2 to 1/4 of the price in fact. But then there are other alternatives you could test that are even cheaper, because this is the cheapest you get for HUMAN consumption, but horse electrolytes for example are a thing, and they can be as cheap as 1/2 of the price of powdered gatorade. So the cheapest possible? You could get down to a nice 8x cheaper than what you got in this video, and if you are able to make a deal wil a company for really large orders, you could go down as much as 10x cheaper, we are talking less than 50 cents per litre here. In fact, horse electrolytes when bought in big bulks, like hundreds of gallons, can be as cheap as 10 cents per litre! Ridiculous.
A quick tip, If you want JAPANESE drinks such as Pocari, you can ship from the Philippines Instead (where i live), It Is SUPER cheap here and I’m sure the shipping Isn’t as expensive either! Cheers!
Bro made the definition of a Mc donalds
As a cell biologist, I'm fascinated. It seems it’s time to seriously diversify the contents of the refrigerator in our laboratory.
You can bring red gatoraid to work, dilute and have some left over for your own mad scientist creations!
Also...Our budgets are not unlimited((((
no sir tax-man these energy drinks are 100% for Science!
Monster Energy meat next
@@calamatica To be fair, it's not the labs that think the budgets are unlimited, it's the purveyors of the materials used. :-(
I do love that thought emporium is functionaly a crowd funded mad scientist
A new and more sustainable form of mad science.
I should not have read that while eating b/c I nearly spat out my food cackling 😂
He makin zombies for sure
you've created him ! Is he Doctor Frankenstein or the Frankenstein Monster ?
@@krash2430 Both
I work for a college and one of my jobs is to order supplies for medical labs. The reason for ordering expensive supplies is because we are state funded and to remain compliant, everything has to have a justification. If we were ordering tons of Gatorade instead of DMEM it would be hard to justify that a sports drink is being used for a lab setting. Additionally, there is oversight bodies that come around to ensure things are being conducted properly, so if a lab has a bunch of Gatorade laying around where there isn't supposed to be food and drink the school can get penalized and fined. Also, many departments receive annual budgets that are adjusted based on usage, so if one department does not use their full budget, then their next yearly budget gets reduced and any remaining funds are taken away and redistributed across the organization. So, departments tend to buy premium supplies and equipment to use up their budget in hopes to have a larger budget the following year, or at least, maximize the usage of their budget.
Damn this sounds just like how it is in the military, I was a cardholder and we had to deal with the same issue
Imagine punishing people for staying underbudget and then wondering why there's so much wasteful governmental spending.
Why not just pour out the gatorades into a labeled container
@@tacticaltaco7481 fraud
edit: If you claim it's not gatorade
@@tacticaltaco7481 or you know..get people to understand that its being used for lab.
One important thing to keep in mind, these cell lines were started and maintained in DMEM with FBS. This means that cells that preferred DMEM and FBS thrived, while those that didn't died off. With rapidly multiplying cells, it would likely be quite feasible to gradually wean the cells entirely off of DMEM and FBS and onto a substitute. This may be much easier than trying to generate cocktails of growth factors.
Another key point is that the cells themselves often make the hormones they want. This is why many cell lines prefer partial media changes rather than complete media changes. This way, the cells always get exposed to most of the hormones they've generated.
Huh... I don't what you're saying but I kinda wish I understood more of what you are talking about now
@@codiserville593 I think they're saying that we could get the cells to evolve so they could survive in pure gatorade
They should definitely try and see if there’s a way to fully transition a cell line to a cheaper medium.
@@jacobwiens659 For sure. The cost involved in obtaining the growth media are one of the biggest obstacles to bringing down the cost of production. (The cost of electricity is another, but we already have a lot of information on how to solve that)
@@goo894 well alright thanks for this possible translation. It's helpful
future racism will be about if you were a red gatorade test tube baby or a blue gatorade test tube baby
"You're one to talk! Mr came from egg juice"
Sounds an awful lot like someone out of blue would say.
well at least that makes more sense then the racism we have today!
such a vitamin water thing to say 🙄🙄🙄🙄
More like dark bear or white bear.
I lost my shit when he flawlessly segued into making meringues. Subbed
Same 😂😭
Absolutely stunning transition 😂
same hahaha
This is giving "how much sawdust can you put in a rice crispy treat before people notice?" And I love that.
Yeah, it’s just “how much green dekara and coconut water can you put in a Petri dish before cells notice?”
although this is the opposite of the sawdust in a way, because adding more Green dakara makes it more tasty
A very bad analogy. This has nothing to do with thinning out a product, it's a new way of producing meat without killing animals.
@@skunkjobb I wasn't making an analogy I was making a goofy comparision
@@skunkjobb It's _LITERALLY_ thinning out a product. Did you not catch that they are using the Gatorade and other drinks as partial replacement for the cell media? Or all that various other stuff as partial or complete replacement for the serum?
Ah, splendid, we're getting closer and closer to finally finishing the ages old task of creating a Homunculus.
The medieval alchemists would be proud!
Let's not crush this one with a book
homunculus is easy, though. Test tube babies already exist.
That would be a fucking insane and I want to see it
@@Flesh_Wizard If it behaves, and doesn't spit acid at its creator.
Haha! Around 13:00 I was like, "Wow, adding sugar... ha, it's like he's making a meringue" [puts into piping bag] "Oh..."
This had me lol
It's crazy to think that common grocery store items could potentially replace some of the most expensive elements in the lab. Very eager to see where this leads to, and how it could potentially revolutionize lab grown meat.
Not entirely. There's almost always going to be minor or major compromises. You might be able to filter or separate out some of the undesirable elements, but every bit of that effort eats into the cost/time savings of using the retail alternative in the first place. But if something along that cheaper price point spectrum works "good enough" with 99.8% or whatever effective substitution, then yeah.
For example, Thought Emporium mentioned two differences: the sports drinks significantly dropped pH balance out of the optimal cell survival range, and some trace elements in DMEM are situationally important for stuff like growing bones. I'm no cell biologist, but I'd imagine there are more nuances like these that they didn't mention.
Another big one is quality control and precision to prevent variation. Two units of the same sports drink from different bottling plants or batches in the same plant could have a huge 0.2% variation in concentration of some elements. Not enough for a consumer to taste more or less salty, but could be enough to throw a precise lab process out of whack. A lot of the extra $100s of cost is for processes that ensure these error margins are orders of magnitude smaller.
So the discount sports drink doping might be good enough for growing boneless McRibs or chicken nugget paste, but not for pseudo pork chops or beef ribeyes that need more realism. Good budget tier alternative for producing off-brand or generic synthetic meat, while the top quality synthetic meat will cost 2X more just to add the necessary 0.2% trace elements or whatever. Just like current consumer product price tiers.
Or the discount stuff could work great for pumping out lower cost protein feedstock to feed to actual animal livestock that's raised for real meat. Not as environmentally or cost optimal as directly growing a high quality vat steak, but maybe it would be an interim technology that cuts cost inputs and greenhouse gas emissions by 10% or something, per pound of real beef produced. That's basically what industrial agriculture has been doing at a lower tech level for the past century -- grinding up undesired wild caught fish bycatch and waste parts from farmed fish to make fish meal, which they then mix into feedstock for more farmed fish or other livestock. Same for waste parts from butchered cows, pigs, chickens, etc that are fed back into the system to feed new animals. (and yes, this has been a source of increased system vulnerability to contamination, disease, and things like growth hormones recirculating back into the system as it approaches both higher efficiency and a slightly more closed loop of production)
Lab grown meat ..?
Dont you mean DIY grown meat ? 😁😆
The reasoning is because those expensive mediums are specifically tested and the ratio of contents confirmed to not have anything additional in there to not throw off experiments.
It’s the same reason anything “aircraft grade” is expensive. It’s also the same reason NIST sells peanut butter for like $400.
You’re not paying for the item but for the paper trail to validate your results.
This is especially important in healthcare research in the case of any kind of side effects or other issues later on.
Stuff like this is definitely great to know, especially if you’re just doing some preliminary research into a topic, but if you’re publishing a paper then you should be using standardised ingredients/processes.
you can't make me eat it
Wouldn't be the first time something like that happened. A while back a lab tested using the old Shrinky Dinks toy to create patterns for use in microfluidics. It worked.
Pocari sweat can be found at most Asian markets both in the US and Canada, with many online retailers also shipping it from local warehouses. No real need to have it imported. Also it comes in its original powder form if you're worried about shipping weights. But all in all its effectively a Japanese clone of Gatorade made by Otsuka Pharma.
Me rn in Singapore chugging the $1.50 (usd1) bottle of PS
Also yes I wanted to mention they come in power form. I remember being in police cadet corps and our outfield ration included the powder.
We mixed it into our water bottles that were brewing in the hot sun, and chugged it - horrible but delicious.
Also had to make lunch using some cheap off-brand instant noodles, and I think some folks forgot to pour out water into the mestin for the noodles before mixing the PS powder. But anyways we opened a tin of sardines in tomato and threw that into the mestin of noodles and honestly, pretty good!
I wondered why he imported it when I can get a bottle of pocari from a local asian store in London for a few pounds. I'd be surprised if you couldn't also in Canada
@@theendoftheworldhasbeenqui2485 honestly it was probably just because its the best way to make sure you get the exact same product. yes its almost guaranteed to be the same locally but it might not be. Also i wonder if the powder might not be better because you could adjust the concentration if need be
@@ztheo2280 In hindsight (yay, I can see clearly now!) the powder form probably would have been better. Still... next cycle?
Since coconut water performed relatifly good as a FBS replacement, maybe try turning other sorts of nuts/seeds into some sort of seed juice. Combining animal and plant produce could also be a way to broaden the nutrient composition, like mixing eggs and coconut water.
The missing ingredients are obviously Sugar, Spice, And Everything Nice, and *_CHEMICAL X_*
I'm pretty sure sugar is already included.
And then the perfect little girl was born
@@darthplagueis13I can’t imagine being satisfied with the sweetness of a Gatorade, tbf. they’d be majority spice and chemicals which is something but isn’t the ratio to be power puff girly enough (according to my own conjecture let’s be real for a sec lmfao-)
@@rose.isnotavailableerm huh uh ok 🫤 🤔 🙄 👍
we're trying to make something to eat, not give it the right to vote
Please call it "Brawndo Serum, it has what cells crave"
This is amazing and absolutely what it should be called.
Clicked on this video expecting the Idiocracy references...did not disappoint
The plan was always to call it "brawndo: the cell growth mutilator, custard flavored"
@@thethoughtemporium Perfect, let me know if you want me to design the shirt :)
@@thethoughtemporium the neuron optimized version can be Braindo
"Sprayed down with alcohol before going into the hood" is a terrifyingly close statement to myself
hahahah
For processing the eggs, take a look at what was used for “albumen print” photographic processes. The literature is not the easiest to find, it’s mostly in century old photographic journals. The goal was to have a clean and optically clear protein gel into which silver nitrate grains would be suspended.
Prep for albumen print is basically what was done here. Take albumen, whisk till stiff, leave overnight, and filter out solids, 🤷🏾
@@rahulsharmajammu Ah, glair! Seems to be pretty useful stuff for more than just illuminating manuscripts and gluing books.
A few friends in my department (USU Biological Engineering) are working on creating an FBS substitute using subcritical water hydrolysis. They take algae and put it under high pressure and temperature to break down the algae into metabolites cells can use. The goal was to produce a vegan media that Upside Foods could use for cultured chicken. Pretty neat!
That's amazing
Hopefully it can be used for all sorts of cell culturing applications beyond the private sector... And that the recipe and protocol is open source
so a pressure cook it to a paste then feed it to vegans, sounds like the pink sludge in fallout
@@Blimsky I am a vegan, and I approve of this message.
Show them this video.
Chaos as they realize gatorade works
I think mcnuggets are already made like this
🦘
Rat meat
Nah they are mixed meat ground up
@@drakologarnus7248 Swan foot and crawfish toes
"Milk is just purified blood."
I guess all mammals are purified vampires.
I mean-
just made me think of Dracula biting someone's booba . lady " help Dracula keeps biting my nips "
bruh...
@@quaxicron no no, they have a point
That's why I don't drink it.
Using Gatorade of all things to grow meat, just amazing. It just feels like an april fools joke or onion article "Mad scientist makes breakthrough cost reduction in artificial meat production, Sponsored by Gatorade".
Idiocracy was up to something, but it was not the plants but meat which craves electrolytes 😂
BRAUNDO, IT HAS WHAT MEAT NEEDS, IT HAS ELECTROLYTES
@@LocusNevernight WHAT ARE ELECTROLYTES, DO YOU EVEN KNOW.
@@GammaRayven 😟
“What do you get when you mix eggs,milk,Gatorade,and nutritional paste” school lunch
"After several hours, Joe finally gave up on logic and reason and simply told the cabinet that he could talk to cells and that they wanted Brawndo"
When will you grow the doom playing brain in Gatorade
ultimate gamer brain
I'm gonna guess "as soon as we figure out how to make it actually work"
In fact, i would assume there's already at least some footage recorded of testing and such
If you grow it in Gfuel it will be esports ready.
@@AageV PERFECT
It's got electrolytes!
It's got what brains crave for.
0:08 YOU GET A VERY DISGUSTING CAKE!!! 🎉
🔥🔥🔥🔥🔥
Yeah.
Meat cake.
Disgusting meat cake.
Dude did not grew meat, he grew a pure gamer
can't wait to have a gamer burger one day
Gamer nuggets
I made a gamer grape????
Ew
Perfect for the Doom Brain Bot
If this channel has taught me anything it's that biochemists absolutely fkn love E.coli and use it a lot more than the average Joe not in the know would ever expect
That's because they reproduce every 30 minutes, they are crazy efficient.
Yep from what I've seen is since they reproduce so fast, they can quickly see any effects, changes & then go from there.. But I'm no certified scientist
there are a plethora of different strains, some of which naturally live in the human digestive system. Only a handful are harmful to people.
@@am529 I know I'm more so just commenting on how E.coli is used far more in biochemistry than anyone not in the know would ever expect.
I for one didn't know until I stumbled on this channel just how much it's used in this field of work.
They're just overall pretty chill dudes. Very relaxed growth requirements, readily uptake DNA if zapped and/or sauna-ed a bit, don't stink to high heaven, they even come in nonopthogenic!
OMG i love this channel. This is my first video but I'm instantly in love. How have i not found this sooner.
“A cow is just a machine that turns grass into milk.”
The design. Its very human.
it's very human,and it's going to ask you to go skatebord
@@cyberious.asdfmovie refrence?
@@DanTDMJace very much so
The design is very cow
same with every other animal. they are all just automated machines.
new alchemy video just dropped
Next project turning lead to gold
@@adengoldstein5130 ...using mushrooms and baby cow juice
@@adengoldstein5130 wikipedia/Golden_rain_demonstration
If they whip out a large metal armor, we better be very worried
Next project combining a dog and a little girl
13:24 I don't know why, but I like the casual swearing in such an educational video.
About 25 years ago when I was a student, I was chatting to a biochemist about food and they mentioned that they'd never eat a custard-filled doughnut, because egg custard is a great growth medium and she'd seen how well it works for bacterial cultures.
So who's the winner? Myself, who has got to enjoy custard filled donuts many times with out negative consequences? Or the person that cut themselves off from experience that cos fear of bacteria
The comment count is already nearing a thousand so I don't know if anyone reads this, but wouldn't it have been more beneficial to use carbon dioxide or some acid that you could later precipitate out, to separate the whey from milk?
Vinegar (mostly acetic acid) is a weak acid with small molecular sizes that can pass cell membranes, so even after neutralizing the pH, it would keep reacting inside and outside the cells and mess up things. At least that's why one should never adjust aquarium water's pH with weak acids. Doing so could easily end up killing the fishes. Only use strong acids (nitric, sulfuric, muriatic) for that, which leave chemically more inert acid residues.
Not sure if any of the aforementioned ones would be suitable for this purpose though. That's why I suggested either carbon dioxide (at least it's the easiest to obtain and apply with sodastream, not sure if there are other acid forming gasses that have high vapor pressure) that could be mostly evaporated from whey by bubbling the solution with nitrogen gas (pumping just air would remove the CO2 as well, but it might also oxidize something important), or maybe there's some suitable acid that would precipitate during the neutralizing process, like oxalic acid + calcium hydroxide.
Just the first few ideas that came to my mind.
yeah acetate ion in humans is toxic so i cant imagine having a bunch of it floating around is good for the cells
Now i want to see what happens if i soda streamed some milk xD
@@Suriel-e5g You'd get lots of foam, and coagulated proteins separating from whey. More importantly, I'm looking forward for your taste report, which is the, erm.. more interesting part 🙃
Dilute sodium acetate is extremely benign. It's used in blood transfusions and neonatal care, so it's probably close to harmless to cells.
Could use dilute HCL. Make some more salt.
11:23 yeah that sentence just ruined cereal for me for the rest of my life...
Wow, that felt like 6 minutes. I was wondering "wait that's it??" as the video ended. You sure made it entertaining!
Scientists around the world: Let’s use animal cells to develop treatments for patients
Thought Emporium: *GATORADE MEAT*
Thought Emporium is the mad scientist the world needs more of.
honestly the high cost seems to be one of the big barriers to practical lab grown meat, not to mention any other lab grown cells, so pretty useful
@@porteal8986 Exactly. An imagine being able to supply all your meat at the cost of some gatorade and an egg (so about $2 instead of the normal $15)
@@NoAIStudios we re about 50 years away from every home having their own meat synthesizer in the basement that you just need to fill the vats with gatorade and eggs every month
Imagine being the Gatorade sales rep making the massive sale to a laboratory to find out they’re using the drinks to generate meat lmao
"Feed me, Seymour" unlocked a DEEP memory with that cut lol
I'm happy that you looked into this as most organisms aren't too picky about what they ingest to survive and most of what we consume is either organic or derived from something that is.
Like you said in the video: the biotech supply industry intentionally runs up the prices of otherwise inexpensive compounds simply because they know they can due to the grant money flowing into universities and other institutions.
Without people working to innovate outside the current system: very little progress will ever be made on things that major corporations aren't interested in. Just the same as how the home computer hobbyists brought about the innovations needed for a truly digital age: biotech enthusiasts will need to learn how to progress outside of the (mostly closed-off) mainstream in order to make real progress in things.
I used to work in pharma, and we said that companies slap an extra 0 on the price tag for the industry. A sterile pack of 15 IPA wipes cost $50.
Yup, very often in the economy the most profitable solutions aren't the most innovative ones but rather the most stagnant ones. No need to waste money developing a new solution (in multiple meanings of the word) if you can just keep forcing people to buy the current one.
Fun fact: you can actually buy pocari sweat as a powder for dillution, I'm pretty sure that would cut down a little on shipping.
A little?
@@thehuntermikipl1170
just dilute the powder in your medium, the powder is way stronger than whatever they put in the bottle
@@_Circus_Clapped_ don't troll
8:49 I'm pretty sure the lab supplier sells them like that for consistency across experiments for being able to replicate experiments from papers. It's like how the ISO standard tea is like 500 dollars for a very bad and bland tea.
That's 100% it, they pay to remove any variation factors. Same goes for most lab stuff.
Have no clue but tend to agree. Not required for exploratory stuff like on this channel though :)
Not sure who came up with removing all variations in order to 'baseline' ; it's a monocultural bland paradigm and actually eliminates any spice which extends life and expands consciousness. We need to understand and implement the interaction between rosemary, cell growth and antibacterial properties for instance as this among others can play a vital part in organic growth overall
@@jonhinman2471 Removing variation is the only way to be sure your results are the the product of the experiment and not some outside force. I've never seen anyone argue against that. Then in the experiment you can make as many/much variations as you want.
@@jonhinman2471 It's just meant for running experiments, that's not meant to be a final product.
Why are academically interesting videos being demonetised whilst Scamverts are STILL being pumped into my home by TH-cam?
Because TH-cam makes more money off of them, so of course they allow it.They are both scammers after all.
So true
The more educated people are, the less likely they are to click on scam advertisements
Idiots click on adds and generate revenue. TH-cam has no interesst in actual humans. That's why channels aimed at children make 100x more money then channels aimed at adults.
Adults have ad- and/or sponsorblocks and mostly don't click on ads. Kids do.
Can't sympathise with AI art users, sorry.
17:57 Egg yolk... not yoke. A yoke is the wooden beam they use to keep oxen together when pulling wagons. I just couldn't keep my mouth shut.
really would not have expected beer to do so well...
Much like your average dad over 40, cells just love beer
it's what secretly keeps humanity alive for millennia
I mean historically speaking wine, beer, and spirits are some of our oldest inventions.
It's basically yeast broth, especially since the way they used it massively dilutes the alcohol.
Brewing beer is cell culture. The main difference is that the spent media is the desired product.
I'm a little confused - wouldn't the most important test here be a control with sterile water diluting the DMEM? To see if the alternatives are either really helping (like DMEM) or just not hurting anything while enough DMEM is available. Sorry if it's a dumb question.
Nah that's a good point. If DMEM can be diluted with distilled water and still work well, that's an instant drop in costs cuz every lab has access to distilled water.
Heck, AC units make it as a side effect of cooling the air
Actually I think your entirely right! I hope he responds to this comment
Could be, but the 100% version with replaced fbs does remediate the results somewhat,
Although yeah the data is better with dmem 0-100% with reverse osmosis distilled water dilution, it's not that complicated to do too,
However the research from japan in video is probably more complete,
If my bio knowledge hasn't failed me, I think that's a negative control: a group that doesn't receive treatment and isn't expected to produce a result.
@@jetison333you're*
This guy is the modern equivalent of a medieval alchemist at this point. Do lead to gold next
Great video, but I also wanted to add some information about university research, for anyone interested
1. When getting grants Universities first take a portion for the use of facilities, this can go up to 50% but I believe 20-30% is standard
2. Assuming your lab isn't big enough for some major lab equipment like an NMR the university often has a communal one...that you must pay to use
3. The lab also needs to pay for graduate student tuition and wages
4. Now that the majority of the money has probably disappeared you now have to deal with whatever miscellaneous costs appear, maintenance of equipment, purchasing chemicals, traveling to scientific conventions
Academia is basically a black hole that devours money
Such a shame. But it is the world we live in, and money seems to be the best tool we could've come up with. It's both one of my favorite and most hated inventions, haha.
Yeah it can be a pain, my advisor basically doesn't have free time with the constant grant applications he has to file, granted it is a fairly large lab
@@ritishifyThis is not an issue of money itself, but of equitable relations. Universities consistently choose to exploit their facility, staff, and students because they can and are incentivized to do so.
@@jinmushui1soul I see. Although I still think that more money would probably radically change things, I guess the issue is more about the management side of things as well... I hope the situation gets better soon
@Dylan-ln6qt
1. When getting grants Universities first take a portion for the use of facilities, this can go up to 50% but I believe 20-30% is standard
HAHAHA, it's over 60 at R1s. I think Duke is ~60, MIT is around ~65, my university is also around 60-70.
Holy shit the concept is freaking genius
If this works on large scale
And is refined
The cost of animal tissue culture will reduce by a significant amount
The cost might get reduced, but the market price will stay the same. That's how it usually works.
Nobody wants lab grown meat
People have already figured that out and started investing serious money. Now people are debating the Morality of it.
@@chriswheeler6092 raising and killing a cow is a lot more immoral than growing meat. One requires death and suffering the other doesn't, and no I'm not a vegan.
@Ger954 aka the future
Now we need the reverse study: how much Gatorade can you replace with dmem before gamers stop seeing advantages
Will dmem become the sports drink of top athletes?
Lol
Guys are really about to put out an open-source generic brand DMEM.
I'm interested now how Aquarius, another Japanese Pocari Sweat competitor, would do. There's also apparently now a "new" Green Dakara, would be interesting to see if it does any different than the regular Green Dakara.
I can help answer part of why the nutritional yeast did so well: it's literally just dead cells. When we brew, we use yeast nutrient, which is honestly just mostly dead yeast. In fact, a quick and dirty way to make yeast nutrient is to just boil a packet of bread yeast for a few minutes. The dead cells contain a lot of the necessary building blocks for new cells to grow, for obvious reasons.
It's really crazy to me that any lab can just casually say they can order some DNA to get printed. Imagine telling someone that 100 years ago.
Not really open source, those brands are still proprietary. But cool af
DNA? Wassat
Yeast cannibalism, lol!
Yeah but that kinda spooks me about bioterrorism, realistically while it may not be full on Tom Clancy's The Division tier, the idea that someone could just begin making designer viruses has got to be about one of the most terrifying concepts in the history of humanity also.
You guys have to try the medical grade Gatorade "pedialyte" it's even available flavorless from most pharmacy.
Hearing the phrase "meat laser" shook my entire world in a way previously not imaginable
"No John, your not allowed to be making meat out of Gatorade in the garage"
I was reading papers some months ago looking for replacements for FBS growth factors and half gave up. Never thought of eggs and milk. Genius.
The joke is on you, providers have been using 70% gatorade on the medium they sell for ages to increase the profit margin.
that'd the make final mix 91% gatorade, and it still works well, you could probably get away with homeopathic concentrations of medium at that rate
@@chyza2012 Homeopathy is a profitable business, expanding to grow mediums.
"When you think about it, milk is just purified blood" is NEVER a sentence I expected to hear
So are tears.
What? That's ridiculous. It's nutritionally fortified sweat!
Don't think about the circumstances for the cows to produce milk then.
Milk is just fancy sweat. Much more tasty than normal sweat though, luckily.
@@feuerling How DOES normal sweat taste?
Just finished work and nearly fell asleep part of the way through. Woke up to the meringues and was VERY confused
The project of growing a complete gamer on gatorade is ongoing I see
With regards to using milk, about a hundred+ years ago physicians did try using milk as a blood replacement for transfusions. It didn't work, but I thought it was interesting to consider that other people throughout history were on a similar path.
You can use coconut milk a blood plasma substitute in a pinch
hahahha I was like, he's making meringue, then he made meringue. xD
Buddy you could have found a local Asian market for that pocari sweat & green one.. But props to burning that money for science!
Not everyone has a local Asian market. I know my town sure doesn’t, and it’s the second biggest in the state I’m in. The most Asian thing I can buy here is like… lo mein noodles from an actual Asian restaurant and offbrand pockys from Trader Joe’s
Thing is, Thought Emporium is a Canadian channel, and outside of major cities it can be very difficult to find ethnic markets that sell stuff like that. Even within the shops that do, it can be difficult. I know here in Manitoba, there's a store in Winnipeg called Oomomo, it specialises in Japanese products. I have not once seen Pocari Sweat or Dakari Green there, but even if i had the stock changes with whats popular in Japan. but Japanese products in general can be hard to locate here. Most Asian markets i've seen cater more towards Chinese and Filipino products as we just have a much larger population of Chinese and Filipino immigrants than we do Japanese.
@@solofdragons6446inside London, Ontario, there are a couple of Asian Markets, and I'm decently sure they have those there, if he lives nearby or wants to make the trip, it's a good idea to check.
@@solofdragons6446He’s in Montréal, Pocari Sweat is 100% available here on some japanese/korean supermarkets
@@solofdragons6446 Order online.
its so cool being able to watch these videos now having done cell culture in lab. this channel was such a huge inspiration for me over the last six or so years
This was a fascinating video. Although not currently studying in the field of biology, I am curious to know if a symbiotic culture like Kefir could make milk, or similar products, better growth media. Anyways, great video as always. Thank you for making such content free, it's been a very long time since I've been to a genetics lab as a student and I miss those days. Good luck with every project.
I was lucky enough to work in a chemical plant as an operator, yhe company was the type you'd take a concept to, and they'd do the R, D & P... and never before in my life have I been more regretful than then... the fac I didn't study chemistry GUTS me.. or any the sciences tbh.
Literally EVERYTHING is chemistry. And it blows my damn mind.
blud is bluffing hard with that grammar, he did not work in a chemical plant yall.
@@StoneBox_761a He was just an operator making the shine, the rest of the gang was doing science on improving the taste to sell it for more.
@@LordDragox412 regardless, i dont think he worked there with that grammar, the requirements to be there are immense.
@@StoneBox_761a He used to have perfect grammar, but then he drank some heads. That's why he's regretful he didn't study chemistry in middle school. Quite a sad story.
Fellow biologist here, I think this should be someone's master's thesis or student project and it is showing promise to be a startup one point. A big however, the only reason I believe that DMEM's can't be replaced by homemade recipes in academia is not the growth issue, more about replicability of the experiments. It's already so hard to follow protocols even though you use the exact brand, the exact amount indicated in the paper. Other than this, let's break the monopoly of lab suppliers lol.
Maybe the industry should move away from proprietary formulas to standardized formulas
@@kokofan50 as the video says, more or less each DMEM contain the same ingredients however such small differences may create certain issues. The point here is the homemade recipes will complicate the issue even more and academia will probably won't like it.
Hell yeah, let's break those price gouging monopolies and cartels that make research unnecessarily expensive!!!
Also, I agree small differences in cell culture media do cause issues. But in a hypothetical situation where labs are making their own DMEM from the same recipe, I believe from my experiences in cell culture that variability that already exists due to differences between lots of FBS is way more significant than the likely differences between homemade batches of DMEM substitute. I hope that sentence made sense.
I’m actually doing a project on this! I’m a 11th grader in Germany, and for my finals, I’m doing a presentation testing accessible methods for growing tissue cultures. It’s part of our final-year research component, where we’re encouraged to choose topics with scientific or social relevance. This video was what inspired me!
"a better solution"
You didn't pause for laughter, but I still caught it and appreciate it.
Note to self: the first step to my genetically engineered catgirls project might be gatorade and beer.
Honestly it's 100 percent possible to do so do what makes you happy!
For a moment there I thought those cartons said "Concentrated concentrate" I was thinking "That must be some INCREDIBLY concentrated...whatever the fuck it is!" 🤦😅😑 I need sleep, but AFTER this awesome vid!
I was concentrating on the concentrated concentrate too
Man, you're freaking awesome! You make biotech so easy! These videos are awesome! But I do miss those older videos where you'd teach us making diy mods or even a whole new piece of equipment, like the nanodrop style spectrometer!
bruh 13:20 was such a genius prediction from whoever edited or scripted the video!
i literally zoned out and got caught spot on, i enjoyed that !! thats a whole different layer of humor
Months of patreon support to pay for a teaspoon of baby cow juice, worth it.
gotta love the Princess Bride reference. I will be hanging around for when the flesh terminator is ready.
I think testing electrolyte powders would be an interesting next step, there are unflavoured ones that can be purchased in bulk for very cheap( $20 for 250L). Would remove any additives from the equation.
At that moment just go for pure additives. Electrolyte drinks are mostly salt, sugar and flavour, sometimes with a small amount of potassium chloride
@@brylozketrzyn Proper rehydration formulas often contain BCAAs and other amino acids as well as
@@Aqoric more complex ones for sure, but we can get aminoacids from many sources. It is about balancing nutrients and hormones.
@@brylozketrzyn Absolutely, my original point was simply for large scale production it would likely prove cheaper
Every day, I get closer to my clone army
you should probably throw those tissues away
elsewhere in the world, someone's working on a droid army
@@adora_was_taken that’s my buddy vin, I sure hope we don’t have conflict in the future
@@tr3vk4mdidn't say clones of ME
xD that Idiocracy joke I was just waiting for it and You didn't left me hanging
God I love how much detail they go into 😭😭😭 take my money just don't stop making this amazing insane content
I guess feline bovine serum is like the real life equivalent of LCL from Evangelion.
@@azertyQthe cat cows are taking over
MooYow
@@azertyQ Wow. That's hilarious, how did that get in there? I'm keeping it!
Yoga serum, but only the one pose.
This gives me great hope in a future that I was taught to anticipate in the 90s… one that I’ve long abandoned
You absolutely killed me with the bit around 13:25. Top notch
At least 2-3 states have, or are about to, ban the sale of cultured meat products. So what we need is a simple, affordable "kit" to grow meat at home. Something akin to gardening, mushroom cultivation, or home brewing. I feel like we're watching the development of such a kit with every new video here! I would love a simple kit/machine where all I'd need to do is subscribe to some kind of service for regular deliveries of raw materials, nutrients, etc., plop those into a machine like an inkjet cartridge, and grow cheap, infinite steaks at home.
Also, kindof shamefully curious to sample my own cells and grow some my own "long-pork-belly." Honestly, I bet I'd be delicious...
I don't think that will ever fly because it's not just the "affordable kit" that's involved in this. It's also all the equipment required and the sterilization and handling protocols required to grow meat tissue in labs.
This team is trained in that area, and even then they're prone to mistakes because that's how it goes. So letting the average Joe meddle with this sounds like a good recipe for an outburst of salmonella or something like that.
I'm not a chemist or a biologist, but with my experience in IT and programming I can attest: the hardest thing to do is making your process user-proof. Someone *will* find a way to screw things over, and in the case of foods, the responsibility falls back on the distribution company.
Wait, let me guess: The states trying to ban this are also full of right-wing, book-burning, cucumbers?
@@sparking023 Off the top of my head, I'd ask if you knew anyone who ever sued hasbro for their kid getting food poisoning from an Easy-Bake Oven cake.
Or, alternatively, sued betty crocker after using unrelated salmonella-infected eggs in a cupcake batter and let their kid lick the spoon.
It seems to me that whatever products would be made of a kit like this would fall under the same kind of legal classifications. As long as the obvious, instructed, and prescribed usage of the kit and its supplementary products is safe, user mishandling would be seen as exactly that, and not tie back to the kit distributor.
@@sparking023tbf (a small amount of) people (non-commercially) can their own food which also requires massive precaution in handling
It wouldn’t be in supermarkets but I could see a world where it becomes a culinary niche as opposed to a home lab niche
Ban the sale? Just how much did the farming lobby -bribe- _ask nicely_ for that?
The thought emporium: carefully researched projects filled with art and science
The thought emporium: meat robot
Your channel is the most interesting thing I've found on TH-cam ever. I wish you success so you can keep working on the projects you want to do and for us to watch them
18:25 "Maybe the cells wanted more uMAMI?" hahahahah😂 so great.
I didn't realize you from Canada, as a fellow Canadian I recommend checking out asian grocery stores, especially chains like T & T as they are likely to carry Pocari Sweat and Dakara. They don't always have it available, as it tends to be a more seasonal import during the summer, but they do carry it!
Finally, I can make my own Meatcubator!
18:32 Try combining the top performers to test if they can complement each other somehow.
Maybe a mixture of some can have a more complete nutrient profile for the cells.
Also another idea to try: "Soylent complete meal powder". It's supposed to be a balanced mixture of most nutrients the body needs.
I'm suspecting that Soylent stuff isn't vegan...
Pocari is available as a powder - not only is it a lot cheaper to ship, but you could adjust the concentration.
God, i love science.
>laberatory technicians developing a nutritional aid for artificial cell growth in labs<
_"Wait... What if we just swap it out with Japanese Gatorade?"_
*>actually works
thank you for your passionate projects that you bless youtube with. this stuff makes me want to go into molecular biology
0:36 , crazy ass line ngl
This was the best video ive seen all month. Cant wait for part 2!
I think the Vitamin water ingredient responsible for the cells not growing may have been vitamin A.
I think it's the kiwi curse. Even jello doesn't work with kiwi.
Came here to say this, I think it's likely.
for the egg bit, you had me completely until "piping bag", at which point I absolutely lost it XD
13:22 you cant do that to me man, its 3 AM, im still drunk and can barely focus on the video 😂🙏 dont make it worse please
Your drunk?
Fr, tome me a second to realize
Aw sweet man made horrors within my comprehension, thanks to this awesome channel!
My brain was already going towards meringue when you started blending the egg whites, the visual just made it easier.
I would have loved to see a control with water or saline used to dilute the mixtures (even though that would probably lead to death pretty fast, but it would kinda put into perspective how beneficial the gatorade ect were)
I thought the same.
A part of me wonders if you can take the best preforming things and basically use it as a recipe for cell production. Theres also a good video by Nighthawkinlight about an experimental procedure to change a lot of variables at once and analyze the results without having to change one factor at a time and doing a ton more work. Doing something like that as a followup could be interesting. Also keep in mind that doing this at home means that you don't need perfect results just a "good enough" standard and I'm sure this can do that. Also maybe in the future you can look at doing it entirely from scratch like you did with the soup.
Dude, you guys are so fucking awesome. I'm guessing you aren't alone working on these which is why I say you guys, so correct me if I'm wrong.
I love this shit.
Agreed, the first sentence of this video is definitely a normal sentence people say on a daily basis.
4:39 with fbs is wild
that was some really cool results. I noticed the best fbs 100% replacements were not also the best at 50%. I'd be curious to see the fbs test with 75% added for an extra data point