Limit Test for Sulphate | How to Perform Limit test for Sulphate | Limit Test Practical | ENGLISH

This video is also available in HINDI Limit Test for Sulphate (HINDI) By Solution Pharmacy • Limit Test for Sulphat...
Download "Solution Pharmacy" Mobile App to Get All Uploaded Notes, Model Question Papers, Answer Papers, Online Test and other GPAT Materials - play.google.com/store/apps/de...
LIMIT TEST FOR SULPHATES
Limit = a value or amount that is likely to be present in a substance
Test = to examine or to investigate
Impurities = a foreign matter present in a compound
Limit test is defined as quantitative or semi quantitative test designed to identify and control small quantities of impurity which is likely to be present in the substance.
Limit test is generally carried out to determine the inorganic impurities present in compound. In short, limit test is nothing but to identify the impurities present in the substance and compare it with standard.
Importance of Limit tests:
To find out the harmful amount of impurities
To find out the avoidable/unavoidable amount of impurities
Apparatus Required
Nessler cylinders, Glass rod ,stand
Chemicals Required
1. Dilute Hydrochloric acid
2. Barium sulphate reagent. Mix 15ml of 0.5M BaCl2, 55ml of water and 20ml of sulphate free alcohol and add 5 ml of 0.0181% w/v potassium sulphate. Dilute to 10ml with water and mix. 0.5M BaCl2 Barium chloride in 1000 ml of water.
Principle:
It is based upon the chemical reaction between Barium chloride and soluble sulphate in presence of dilute Hydrochloric acid . The turbidity produced is compared with the standard solution. Barium sulphate reagent contains barium chloride, sulphate - free alcohol and small quantity of potassium sulphate. The inclusion of the small quantity of potassium sulphate in the reagent increases the sensitivity of the test. Alcohol prevents super saturation and more uniform turbidity develops. If the turbidity produced in the test is more intense than the standard turbidity it fails the test. Otherwise, it passes the test.
Standard compound
1. Take 1ml of 0.1089 % W/V solution of potassium sulphate in Nessler cylinder.
2. Add 2ml of dilute hydrochloric acid
3. Dilute to 45 ml in Nessler cylinder
4. Add 5ml of barium sulphate reagent.
5. Keep aside for 5 min
6. Observe the Turbidity
Test sample
1. Specific weight of compound is dissolved in water or solution is prepared as directed in the pharmacopoeia and transferred in Nessler cylinder.
2. Add 2ml of dilute hydrochloric acid.
3. Dilute to 45 ml in Nessler cylinder
4. Add 5ml of barium sulphate reagent
5. Keep aside for 5 min.
6. Observe the Turbidity
Observation
Barium sulphate reagent contains barium chloride, sulphate free alcohol and small amount of potassium sulphate. The turbidity produce in sample solution should not be greater than standard solution. If turbidity produces in sample solution is less than the standard solution, the sample will pass the limit test of sulphate and vice versa.
Reasons
1. Hydrochloric acid helps to make solution acidic.
2. Potassium sulphate is used to increase the sensitivity of the test by giving ionic concentration in the reagent
3. Alcohol helps to prevent super saturation.
Get in touch with the solution by just clicking the following links-
Facebook Group- / solutionpharamcy
Facebook Page- / pharmavideo
New Channel (Pharmacy Dictionary) / @pharmacydictionary
Instagram- / solutionpharmacy
E-Mail for official and other work - [e-mail]
LinkedIn- / pushpendrakpatel
#solutionpharmacy #Pharmacologyclass #Pharmacognosyvideos #GPATonlinetest #GPATclass #GPATvideos #Microbiologyclass#Microbiology#

[@charithashalini8721]

U will do lead test also mam

[@rizarichuzz1047]

Mam how can get solution pharmacy app in iphone plz reply

[@benjaminsavarabenjaminsava8132]

Little slow explanation if it be very understanding topic to me

[@shahsirbulndsirbuland8319]

Nice lecture 👍🏼 keep it up ✍🏻👍🏼

[@budigevenkatesh4619]

👍🏻

[@PawanYadav-io8bb]

Thnxx mam👍🏻👍🏻❤️❤️❤️

[@YASHPALSINH_1011]

Bpharm is a pera medical line?

[@dyechemlab5968]

How much hcl should be diluted?

[@tepallibhuvaneswari4946]

Keep it going mam

[@THESHIV2.O]

Nice ma'am

[@jayanthmg]

Limit tests are done using nesselers cylinder 👍

[@YASHPALSINH_1011]

B. Pharm is a pera medical lime?

[@solutionpharmacy]

Yes

[@michinanya8561]

Ma'am the title is Limit test of Sulphate but in the content it is mentioned as Limit test of Chlorides. So, I am a bit confused. Can u please clear it.

[@deepakjangra6631]

🌟🌟🌟🌟🌟

[@surajvlogCHD]

Mam apne principle me hcl ki jagah pe kcl likha hai