Thanks for the video, hope the move went well! It's nice to see under the hood on these machines. How much of a difference is there in sample prep/loading between this and the JEOL?
The sample prep is the same (FIB sectioning, ultramicrotoming, drop casting, etc.). One major difference has to do with the need to plasma clean the samples prior to insertion. We have to do this with the Talos, but I rarely need to do so with the JEOL 2100. This has to do with the different electron sources between the two instruments. The JEOL 2100 I last managed had a LaB6 crystal as the electron source, whereas the Talos has a FEG. This means the Talos is much much brighter, and when in STEM mode carbon contamination can build up on your sample quite quickly. Plasma cleaners are very pricey (20k to 75k Euros), so it's good to know ahead of time that if you're planning to buy a FEG TEM, make sure to budget for the plasma cleaner! The loading is similar to other Thermo Scientific/FEI/Philips TEMs. I'll eventually do a video showing how to load the main holders we use at Goethe University. In short, I find it to be much easier to do the loading with the holders from Thermo Scientific/FEI/Philips!
Yes, that’s right. There is a camera (Flu-Cam) that looks at a phosphor screen which we use for alignments, navigation, and collecting diffraction patterns.
Thanks for the video. Very impressive. Hope you are settling in and enjoying Germany and the new job. Looking forward to the future videos.
Thanks!
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Thanks for the video, hope the move went well! It's nice to see under the hood on these machines. How much of a difference is there in sample prep/loading between this and the JEOL?
The sample prep is the same (FIB sectioning, ultramicrotoming, drop casting, etc.). One major difference has to do with the need to plasma clean the samples prior to insertion. We have to do this with the Talos, but I rarely need to do so with the JEOL 2100. This has to do with the different electron sources between the two instruments. The JEOL 2100 I last managed had a LaB6 crystal as the electron source, whereas the Talos has a FEG. This means the Talos is much much brighter, and when in STEM mode carbon contamination can build up on your sample quite quickly. Plasma cleaners are very pricey (20k to 75k Euros), so it's good to know ahead of time that if you're planning to buy a FEG TEM, make sure to budget for the plasma cleaner!
The loading is similar to other Thermo Scientific/FEI/Philips TEMs. I'll eventually do a video showing how to load the main holders we use at Goethe University. In short, I find it to be much easier to do the loading with the holders from Thermo Scientific/FEI/Philips!
Nice video. I guess instead of the viewing screen there will be a camera.
Yes, that’s right. There is a camera (Flu-Cam) that looks at a phosphor screen which we use for alignments, navigation, and collecting diffraction patterns.
@@sherisingerlingthis is a good feature in new TEM microscopes. We can work without switching off room lights