Nicholas, Thanks a lot for your nice videos, now I am practicing FEI Cs-Titan G2, i learn a lot from your explanation although the video is for Talos. I am looking forwards to hearing from your new Cs-corrected teaching video in the near future. That's a great job for all the TEM learners, you are doing a great work. I have recommended your videos to my colleagues. it will help them to understand TEM better. Thanks again
You're welcome; the Cs-corrected STEM tutorial is definitely in the works. I had to spend a lot of time with the instrument to fully wrap my head around it but I'm now to the point where I can do a good tutorial so pleases be on the lookout.
Nicholas, thanks very much for sharing such a nice vedio! After learning from your video, now I can really do the axis alligment in STEM mode! I am so glad tonight and thanks again!
Hello Nicholas. This raw version is really helpful. One thing I feel is that doing STEM alignment is harder while looking ronchigram over a flu screen, especially with uncorrected ones. Looking the ronchigram via a camera is much easier when it comes to aligning.
Hi Rahul: thanks for the feedback that you prefer the "raw" version. It's certainly a lot easier for me to film a "raw" version as opposed to filming and then going back and doing a voice-over.
Hi Cailing: you're welcome. Yes, that is probably the most requested topic right now from my subscribers and I'm finally comfortable enough that I can do it so please be on the look out for it in the near future.
Hello up!! In fact, I just start to use fei instrument from jeol instrument, there are some points I confused, 1. In STEM align, when switch to image plane, just for adjusting coma? I think using ronchigram is more convenient. 2. Usually the button 'focus' control CL2, but when we should set it as obj focus? I think maybe using obj focus is more accurate. Looking forward to hearing from you soon.
Hello Nicholas. Thank you for the tutorial. I want to ask a question about which lens to use for focusing in STEM. At time @25:43, you mention the C2 lens should be used for focusing, and call people who prefer Objective Lens as knuckleheads :) However, I thought objective lens was the highest quality lens there is in the microscope, and therefore should be used for focusing. I would really like some guidance on which lens is the best way to go. Can you please give more detail on the reason behind your choice?
Hi Berk: ha ha, yes; my use of "knuckleheads" was meant to be a playful jab; hopefully, there were not too many people offended by that! There are a few different ways to approach focusing in STEM mode, but everyone usually agrees that the objective lens should be modified as little as possible from the eucentric focus value. By changing the controls so "Focus" adjusts C2, this does not remove the objective lens from the configuration, it is still there and focusing and forming the final image of the probe on the sample and is still the highest quality lens (as you correctly point out). Setting "Focus" to C2 and using the finest focal step setting, you can focus just as accurately as if you were directly adjusting the objective lens. My experience is that the default C2 setting in STEM mode does not have a very high degree of repeatability in terms of producing a focused image, so I tend to lean towards doing the most tweaking to that lens instead of the objective. Of course, keep in mind, this is all pertaining to a non Cs probe-corrected 2 condenser lens system; things are a little more complicated with something like our Themis Z. That being said, discussing focusing options in STEM mode is probably a good topic for a video, so thank you for the idea for that!
Hi Sara: you're welcome. I'm not sure if I'm allowed to extend these to the general public outside my university. I will ask my supervisors before I do my next workshop to see if it will be OK if invite my YT subscribers to attend.
Hi Sara: on FEI instruments you can't remove the C2 aperture, so there isn't anything to worry about in that case. You can only do this on the JEOL instruments and I've never had a problem doing this on those systems. Adjusting the C2 aperture shouldn't affect the gun/filament at all; you would need to adjust the gun extractor/suppressor voltage and/or filament voltage to possibly hurt the filament.
very good playthrough for atomic-scale imaging! Thank you for sharing!
You're welcome; thanks for watching!
Nicholas, Thanks a lot for your nice videos, now I am practicing FEI Cs-Titan G2, i learn a lot from your explanation although the video is for Talos. I am looking forwards to hearing from your new Cs-corrected teaching video in the near future. That's a great job for all the TEM learners, you are doing a great work. I have recommended your videos to my colleagues. it will help them to understand TEM better. Thanks again
You're welcome; the Cs-corrected STEM tutorial is definitely in the works. I had to spend a lot of time with the instrument to fully wrap my head around it but I'm now to the point where I can do a good tutorial so pleases be on the lookout.
Thanks, Niko. It is a really good job. It is very helpful for new Microscopists like us.👏
You're welcome; sorry for the very late reply.
Nicholas, thanks very much for sharing such a nice vedio! After learning from your video, now I can really do the axis alligment in STEM mode! I am so glad tonight and thanks again!
You're welcome, I'm very glad my videos helped you out!
great job Nick.
Thank you.
Hello Nicholas. This raw version is really helpful. One thing I feel is that doing STEM alignment is harder while looking ronchigram over a flu screen, especially with uncorrected ones. Looking the ronchigram via a camera is much easier when it comes to aligning.
Hi Rahul: thanks for the feedback that you prefer the "raw" version. It's certainly a lot easier for me to film a "raw" version as opposed to filming and then going back and doing a voice-over.
Thanks
Hi, thank you very much for your video. Could you please make a veideo about running a Probe Cs- corrector?
Hi Cailing: you're welcome. Yes, that is probably the most requested topic right now from my subscribers and I'm finally comfortable enough that I can do it so please be on the look out for it in the near future.
Hello up!! In fact, I just start to use fei instrument from jeol instrument, there are some points I confused,
1. In STEM align, when switch to image plane, just for adjusting coma? I think using ronchigram is more convenient.
2. Usually the button 'focus' control CL2, but when we should set it as obj focus? I think maybe using obj focus is more accurate.
Looking forward to hearing from you soon.
Hello Nicholas. Thank you for the tutorial. I want to ask a question about which lens to use for focusing in STEM. At time @25:43, you mention the C2 lens should be used for focusing, and call people who prefer Objective Lens as knuckleheads :) However, I thought objective lens was the highest quality lens there is in the microscope, and therefore should be used for focusing. I would really like some guidance on which lens is the best way to go. Can you please give more detail on the reason behind your choice?
Hi Berk: ha ha, yes; my use of "knuckleheads" was meant to be a playful jab; hopefully, there were not too many people offended by that! There are a few different ways to approach focusing in STEM mode, but everyone usually agrees that the objective lens should be modified as little as possible from the eucentric focus value. By changing the controls so "Focus" adjusts C2, this does not remove the objective lens from the configuration, it is still there and focusing and forming the final image of the probe on the sample and is still the highest quality lens (as you correctly point out). Setting "Focus" to C2 and using the finest focal step setting, you can focus just as accurately as if you were directly adjusting the objective lens. My experience is that the default C2 setting in STEM mode does not have a very high degree of repeatability in terms of producing a focused image, so I tend to lean towards doing the most tweaking to that lens instead of the objective. Of course, keep in mind, this is all pertaining to a non Cs probe-corrected 2 condenser lens system; things are a little more complicated with something like our Themis Z. That being said, discussing focusing options in STEM mode is probably a good topic for a video, so thank you for the idea for that!
Great video, thank you so much. Is this workshop? How can we join?
Hi Sara: you're welcome. I'm not sure if I'm allowed to extend these to the general public outside my university. I will ask my supervisors before I do my next workshop to see if it will be OK if invite my YT subscribers to attend.
Hi Nicholas, someone told me not to remove the C2-aperture, it may burn the filament, is that true? Totally what can burn the filament?
Hi Sara: on FEI instruments you can't remove the C2 aperture, so there isn't anything to worry about in that case. You can only do this on the JEOL instruments and I've never had a problem doing this on those systems. Adjusting the C2 aperture shouldn't affect the gun/filament at all; you would need to adjust the gun extractor/suppressor voltage and/or filament voltage to possibly hurt the filament.