Dr. Jack! Im from Brazil! Learning with you! You earn a fan here in Brazil! Your content have been very useful for me in my post graduation! I will work with this methodology soon! Thanks for all!
For human samples a heparinised vacutube is best. For animal work I normally use a heparinised needle (or sodium citrate) then dispense into a 2ml centrifuge tube. It's important to take the needle off before ejecting the blood, if you force it through the needle you will lyse your cells. Then centrifuge the tube at ~500g for 5 minutes, take the plasma off the top, leave the pellet, and run the CBA on the plasma.
Thank you for detailed description. ❤🔥
Dr. Jack! Im from Brazil! Learning with you! You earn a fan here in Brazil! Your content have been very useful for me in my post graduation! I will work with this methodology soon! Thanks for all!
Thank you soo very much for your videos. You demystified Flow Cytometry.
Look forward to more videos.
So well explained thank you so much! Never heard of bead immunoassays before and had to study multiplex ELISA in a quickly way
It was super clear, funny, and informative! Thank you very much!
Very Helpful. Thank You!
Please make more videoes on advance immunological techniques.. this was very good
This was super helpful and easy to understand!
Hello Dr jack, what type of sample tube is most appropriate for blood collection in CBA assay.
Thank you
For human samples a heparinised vacutube is best. For animal work I normally use a heparinised needle (or sodium citrate) then dispense into a 2ml centrifuge tube. It's important to take the needle off before ejecting the blood, if you force it through the needle you will lyse your cells. Then centrifuge the tube at ~500g for 5 minutes, take the plasma off the top, leave the pellet, and run the CBA on the plasma.
Thank you soo very much for your videos. You demystified Flow Cytometry.
Look forward to more videos.