Small Molecule Microarrays for Drug Discovery

The speaker is Dr. Adam Buckle, Chief Scientific Officer at Arrayjet, hosted by Oleksii Gavrylenko, Chemspace Chief Operating Officer.
We discussed the following topics related to the Small Molecule Microarray (SMM) methodology:
- What a SMM is, how they were developed, and how they are used;
- The full process of how SMM screening is carried out;
- The types of target molecules SMM can be used for, including case studies;
- How the technique addresses current drug discovery challenges.
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0:00 Start of the webinar
2:40 Chemspace introduction by Oleksii Gavrylenko, Chief Operating Officer
9:52 Speaker introduction - Dr. Adam Buckle, CSO at Arrayjet
11:42 Introduction to Small Molecule Microarraying
11:58 Overview of the presentation
12:23 Arrayjet’s focus
12:59 Arrayjet products and services
13:51 What is a microarray?
14:43 What is a small molecule microarray?
15:30 Process overview: small molecule microarray.
16:05 Part 1. Small molecule libraries
18:08 Part 2. Isocyanate surface chemistry
20:00 Surface reactivity test for SM binding
22:26 Isocyanate surface chemistry: Why is it important?
23:33 Part 3. Printing: Mercury for Small Molecule Screening
24:16 Mercury only uses inkjet
24:53 Microfluidic sample transfer
25:40 A simplified view: aspiration and printing
26:24 We image every printed spot
26:51 Why Arrayjet and Small Molecule Microarraying (SMM)
28:58 Part 4. Binding assay and hits identification
29:11 Target types for screening assay - Protein and RNA
32:34 Protein SMM: Rapamycin example
34:02 Targeted protein degradation. PROTACs
37:03 Drugging RNA
38:58 Riboswitches as a Paradigm for understanding structural RNA
40:20 PreQ1-riboswitch SMM case study
42:04 Example SMM assay: PreQ1-riboswitch
43:26 Example SMM screen
44:30 Part 5. Validation. Target validation using SPR imaging
48:07 Benefits of SMM
50:34 Summary of SMM
50:42 Q&A session