I have made a home scale very small Bioreactor for growing up yeast cultures of Kveik Yeast for drying and freezing yeast slurry , Its a 1 quart to 1/2 gallon reactor depending on which jar i use. Basically its a ball airlock lid( the kind for fermenting vegetables with a built in airlock) that i drilled a second hole in and put a stainless steel air stone that is hooked to an air pump and it sits on a Stir plate, so, if my understanding of the process is correct this would be a Very Basic Bioreactor. My issue is the feeding schedule for optimum yeast growth and how much sugar and nutrient i should be feeding it daily. I have Fermax yeast Nutrient and I am doing my Grow up with white Table sugar because its a cheap easy sugar source. I am building up a personal yeast bank of Kveik yeast strains for use in Home wine,beer and mead making. So My goal is the highest biomass I can achieve with the limits of my home made set up. My reactor it self is working so far I am just not sure on a rough estimate of how much sugar and nutrient to feed it daily. I dont have a super accurate Gram scale(yet) so I am using measuring spoons and cups at this point At the present time, I am doing roughly 1/2 teaspoon of yeast nutrient and about 1/4 cup of sugar daily in a 1 quart volume of water I was wondering how these amounts look and if i should change them, if so how much of each would you suggest. The yeast nutrient I have is a General purpose nutrient designed for home wine,beer and mead making. My sugar source is currently white table sugar due to price and ease of access. Any suggestions you can offer would be greatly appreciated.
Fermenting: have been using small ultrasonic cleaners for making Aging alcohol Cannabis edibles, Tinctures and Coloidal Silver.I had the Idea to try it on my Fermentation of grain mash. My hopes were to make the mash into smaller particles and increasing surface area. I hoped to increase Bioavailability for the yeast. The 6 gal. plastic bucket I used for it kept blowing its top off as the bubbler was unable to keep up with the new rate of fermentation. I had to resort to taping the lid down with duct tape, Watching the bucket bulge from unreleased pressure and hoping it did not explode. !!WARNING DO NOT USE A GLASS VESSEL FOR FERMENTING IF YOU SHOULD TRY THIS METHOD!! I had hoped to show the increased speed of fermentation in this video. I made two mistakes. I used some pretty old yeast and I did not malt the grain first. Still the control had not even started before the ultrasounde grain had filled its bag full. I believe if I had used a malted grain and newer Yeast the results would have been more dramatic for you to see. I am very new to brewing, only three batches with using ultrasound to decrease particle size the third batch. I did try a sugar wash 4th batch using the ultrasound but saw no changes in the speed of fermentation. . I am hoping someone with a better set up and greater brewing knowledge can experiment with this and show the actual rate of increase. (I believe it to be about 300% or better) This could have a major significance in such a large market as alcohol and fuel.A factor to consider is stirring the grai water mixture in the ultrasound as a heavy layer of grain at the bottom may reduce effectiveness. I used a 60 watt, 40khz ultrasound unit with just one transducer on this video . On the earlier bucket run I used a home made unit with two 40 khz transducers. Finding the optimal range of transducers, frequency , volume, stir rate and length of time I leave to future brewers with more capital and time to find th-cam.com/video/b_mdvsEF3vo/w-d-xo.html
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I have made a home scale very small Bioreactor for growing up yeast cultures of Kveik Yeast for drying and freezing yeast slurry , Its a 1 quart to 1/2 gallon reactor depending on which jar i use.
Basically its a ball airlock lid( the kind for fermenting vegetables with a built in airlock) that i drilled a second hole in and put a stainless steel air stone that is hooked to an air pump and it sits on a Stir plate, so, if my understanding of the process is correct this would be a Very Basic Bioreactor.
My issue is the feeding schedule for optimum yeast growth and how much sugar and nutrient i should be feeding it daily. I have Fermax yeast Nutrient and I am doing my Grow up with white Table sugar because its a cheap easy sugar source.
I am building up a personal yeast bank of Kveik yeast strains for use in Home wine,beer and mead making. So My goal is the highest biomass I can achieve with the limits of my home made set up.
My reactor it self is working so far I am just not sure on a rough estimate of how much sugar and nutrient to feed it daily. I dont have a super accurate Gram scale(yet) so I am using measuring spoons and cups at this point
At the present time, I am doing roughly 1/2 teaspoon of yeast nutrient and about 1/4 cup of sugar daily in a 1 quart volume of water I was wondering how these amounts look and if i should change them, if so how much of each would you suggest. The yeast nutrient I have is a General purpose nutrient designed for home wine,beer and mead making. My sugar source is currently white table sugar due to price and ease of access. Any suggestions you can offer would be greatly appreciated.
Air fillter call part ?
Terima kasih pak 🙏🏻
is it this for tartaric acid ?
add two drops 1:23 oops
I think it's anti-FOAM??
Autoclaving a live yeast culture? Adding yeast to distilled water? Won't that kill it?
Yes the yeast is supposed to be killed. You're using dead yeast as a nutrient medium, not trying to grow live yeast.
The yeast is food.
Fermenting: have been using small ultrasonic cleaners for making Aging alcohol Cannabis edibles, Tinctures and Coloidal Silver.I had the Idea to try it on my Fermentation of grain mash. My hopes were to make the mash into smaller particles and increasing surface area. I hoped to increase Bioavailability for the yeast. The 6 gal. plastic bucket I used for it kept blowing its top off as the bubbler was unable to keep up with the new rate of fermentation. I had to resort to taping the lid down with duct tape, Watching the bucket bulge from unreleased pressure and hoping it did not explode. !!WARNING DO NOT USE A GLASS VESSEL FOR FERMENTING IF YOU SHOULD TRY THIS METHOD!! I had hoped to show the increased speed of fermentation in this video. I made two mistakes. I used some pretty old yeast and I did not malt the grain first.
Still the control had not even started before the ultrasounde grain had filled its bag full. I believe if I had used a malted grain and newer Yeast the results would have been more dramatic for you to see. I am very new to brewing, only three batches with using ultrasound to decrease particle size the third batch. I did try a sugar wash 4th batch using the ultrasound but saw no changes in the speed of fermentation. . I am hoping someone with a better set up and greater brewing knowledge can experiment with this and show the actual rate of increase. (I believe it to be about 300% or better)
This could have a major significance in such a large market as alcohol and fuel.A factor to consider is stirring the grai water mixture in the ultrasound as a heavy layer of grain at the bottom may reduce effectiveness. I used a 60 watt, 40khz ultrasound unit with just one transducer on this video . On the earlier bucket run I used a home made unit with two 40 khz transducers. Finding the optimal range of transducers, frequency , volume, stir rate and length of time I leave to future brewers with more capital and time to find
th-cam.com/video/b_mdvsEF3vo/w-d-xo.html
Botany students here