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- เผยแพร่เมื่อ 16 ก.ย. 2024
- ( www.abnova.com ) - H&E stain is a popular staining method in histology. It's a combination of two dyes: the basic dye (hematoxylin) and the alcohol-based dye (eosin). In an H&E stain you'll usually see both eosinophilia and basophilia: the nuclei of cells basophilic (blue), while eosinophilia is typical of cytoplasmic constituents (pink). More videos at Abnova www.abnova.com
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A lot goes into this. I wonder how someone figured out the alcohol concentration quantities and times needed.
It's all based on trial & error.
the alcohol you're referring is due to "XYLENE removal" process just after deparrafinization...Xylene is miscible w/ absolute alcohol..so use 100% alcohol to remove xylene...then descending concentrations of alcohol..from 90-50% will add-up water from the tissue..."90"% alcohol means there is 10% water in it, 50% alcohol mean 50% alcohol and 50% water . That is where the tissue becomes hydrated..ready for staining...hope u get it.
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@DerSchnurrbart You sure don't. We use an automatic staining machine that dips slides in xylene for about 5 minutes per bath. The gradient of alcohols they use is much more long and involved than ours too. It might have something to do with the specific formulation of the stain they used, but I don't know. This seems more complicated than it has to be.
@TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated.
Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )
this was the explanation I was looking for, thank you for taking the time to explain this concept.
I'm just being started. Let this video be my reference point! Wish me good luck!
why that guy didnt differentiate or blue?
This was...AMAZING!!!! Thanks for the vid!
Thanks, this helped me with my university assignment
I don't think you need to put the slide into the xylene so long when you are deparaffinizing it.
How much time is minimally required to prepare H and E slide for reporting?
1 hour in Xylene is a lot isn't it? 0_o We only do 6 minutes total in Florida.
histobelle may be depending on technician
@@hopehope8597 The tissue doesn't care who the technician is, but maybe the kinds of samples they work with are different
Sir thankyou apke video se bahot help ho jati samjne main ese hi agge hamare liye video banate rhe
Thankyou khoob khoob thankyou
Why the serial dilution in organic solvents?
what is the meaning of two changes of 2 mins each?
it just try which put from PBS to absolute EtOH twice?
That procedure is too long,and I've notice in this video that no diferentiation with 0.5% HCL after hematoxylin was done.
I know that the alcohol is used for dehydration, but why so many different types of concentration? (student :) )
Hello, what are the most widely used fixative in HandE staining method
A. Formaldehyde
B.zenker formol
C. Picric acid
D. Osmic acid
Please help me answer
Formaldehyde
@DerSchnurrbart yes i agree.
is this Harris’s haematoxylin ?
@TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.
may I have your protocol ? please email me to ryansaputra252@gmail.com
check my video on tissue Staining
Soo helpful thankss
Thankyou so much sir
Thanku so much
5 min in xylene is all u need to get rid of the paraffin and just a min each in the other reagents
may I have your protocol ? please email me to ryansaputra252@gmail.com
Fantastic
And easier
Is this how babies are made
Nope you are wrong
@@madhanprasathr247 Excellent, thank you for the response. Unfortunately I needed the answer to this nearly a decade ago...
@@Azander137 sry bruh before a decade i was just a school kiddo 😂
@@Azander137 n
this medicine
how we get sections tissue
saaai tá tudo errado
@MrJazkiller oo ok thanks very much indeed :-)
latest video : H&E Staining | Purpose of staining in histopathology | Human Touch in H&E Staining : th-cam.com/video/WBUriohMkSM/w-d-xo.html
Hello, what are the most widely used fixative in HandE staining method
A. Formaldehyde
B.zenker formol
C. Picric acid
D. Osmic acid
Please help me answer
I don't know the most widely but I used Formaldehyde to fixed gills and stomach of fish.
Formaldehyde