H&E Staining

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  • เผยแพร่เมื่อ 24 ธ.ค. 2024

ความคิดเห็น • 51

  • @MrJazkiller
    @MrJazkiller 12 ปีที่แล้ว +23

    the alcohol you're referring is due to "XYLENE removal" process just after deparrafinization...Xylene is miscible w/ absolute alcohol..so use 100% alcohol to remove xylene...then descending concentrations of alcohol..from 90-50% will add-up water from the tissue..."90"% alcohol means there is 10% water in it, 50% alcohol mean 50% alcohol and 50% water . That is where the tissue becomes hydrated..ready for staining...hope u get it.

    • @fabianryan9192
      @fabianryan9192 3 ปีที่แล้ว

      i guess im asking randomly but does anybody know of a way to get back into an Instagram account?
      I was stupid forgot the account password. I would appreciate any assistance you can give me.

    • @fabianryan9192
      @fabianryan9192 3 ปีที่แล้ว

      @Oliver Trent i really appreciate your reply. I found the site on google and im in the hacking process atm.
      I see it takes quite some time so I will reply here later when my account password hopefully is recovered.

    • @fabianryan9192
      @fabianryan9192 3 ปีที่แล้ว

      @Oliver Trent It did the trick and I finally got access to my account again. I am so happy!
      Thanks so much, you saved my account !

    • @olivertrent9266
      @olivertrent9266 3 ปีที่แล้ว

      @Fabian Ryan you are welcome :D

  • @kikook222
    @kikook222 9 ปีที่แล้ว +43

    A lot goes into this. I wonder how someone figured out the alcohol concentration quantities and times needed.

    • @BestWinnerone
      @BestWinnerone 3 ปีที่แล้ว +8

      It's all based on trial & error.

  • @MrJazkiller
    @MrJazkiller 13 ปีที่แล้ว +3

    @TheRealHarsjan for "Dehydration" alcohol starts from lower concentration to higher then to absolute concentration. Rationale: if tissues are submerged directly to high alcohol con. tissues will become hard or brittle, the outer portion of the tissue will dehydrated quick but the inner part is not fully dehydrated.
    Prior in performing H & E staining, you need to " Deparaffinize " use xylene, then remove xylene by absolute alcohol ( xylene is only miscible w/ absolute alcohol )

    • @yasminali7578
      @yasminali7578 7 ปีที่แล้ว

      this was the explanation I was looking for, thank you for taking the time to explain this concept.

  • @Theonewhoclimbs28
    @Theonewhoclimbs28 14 ปีที่แล้ว +3

    @DerSchnurrbart You sure don't. We use an automatic staining machine that dips slides in xylene for about 5 minutes per bath. The gradient of alcohols they use is much more long and involved than ours too. It might have something to do with the specific formulation of the stain they used, but I don't know. This seems more complicated than it has to be.

  • @ASSHOLELA
    @ASSHOLELA 13 ปีที่แล้ว +6

    why that guy didnt differentiate or blue?

  • @tajmuhammad3715
    @tajmuhammad3715 4 ปีที่แล้ว +2

    How much time is minimally required to prepare H and E slide for reporting?

  • @ho-sl1ej
    @ho-sl1ej 2 ปีที่แล้ว

    what is the meaning of two changes of 2 mins each?
    it just try which put from PBS to absolute EtOH twice?

  • @ManuelGutierrez-zb5xm
    @ManuelGutierrez-zb5xm 8 ปีที่แล้ว +1

    Why the serial dilution in organic solvents?

  • @DerSchnurrbart
    @DerSchnurrbart 14 ปีที่แล้ว +4

    I don't think you need to put the slide into the xylene so long when you are deparaffinizing it.

  • @bellamarie712able
    @bellamarie712able 11 ปีที่แล้ว +6

    1 hour in Xylene is a lot isn't it? 0_o We only do 6 minutes total in Florida.

    • @hopehope8597
      @hopehope8597 7 ปีที่แล้ว

      histobelle may be depending on technician

    • @jatnarivas8741
      @jatnarivas8741 3 ปีที่แล้ว +1

      @@hopehope8597 The tissue doesn't care who the technician is, but maybe the kinds of samples they work with are different

  • @cjcares7815
    @cjcares7815 6 ปีที่แล้ว +1

    I'm just being started. Let this video be my reference point! Wish me good luck!

  • @TheRealHarsjan
    @TheRealHarsjan 13 ปีที่แล้ว

    I know that the alcohol is used for dehydration, but why so many different types of concentration? (student :) )

  • @MrJazkiller
    @MrJazkiller 13 ปีที่แล้ว

    @TheRealHarsjan after removing xylene...you need remove the alcohol from the tissue and replace it with water..so that your stains will have more affinity to the tissue. how do you do this? by passing through series of high concentrations of alcohol ( starts from your absolute to remove xylene) then to 90-80-70 then finally to water...then you can start H& E staining.

    • @febriyansyahsaputra
      @febriyansyahsaputra 7 ปีที่แล้ว

      may I have your protocol ? please email me to ryansaputra252@gmail.com

    • @anirbanmukherjee2811
      @anirbanmukherjee2811 6 ปีที่แล้ว +1

      check my video on tissue Staining

  • @simranpangli976
    @simranpangli976 8 ปีที่แล้ว

    is this Harris’s haematoxylin ?

  • @ricasaluria8821
    @ricasaluria8821 4 ปีที่แล้ว +1

    Hello, what are the most widely used fixative in HandE staining method
    A. Formaldehyde
    B.zenker formol
    C. Picric acid
    D. Osmic acid
    Please help me answer

  • @Pistolerong_Pinoy
    @Pistolerong_Pinoy 13 ปีที่แล้ว +1

    That procedure is too long,and I've notice in this video that no diferentiation with 0.5% HCL after hematoxylin was done.

  • @DavidsonLoops
    @DavidsonLoops 11 ปีที่แล้ว +1

    Thanks, this helped me with my university assignment

  • @AliAzizQualityEducation
    @AliAzizQualityEducation 5 หลายเดือนก่อน

    latest video : H&E Staining | Purpose of staining in histopathology | Human Touch in H&E Staining : th-cam.com/video/WBUriohMkSM/w-d-xo.html

  • @rcamobile
    @rcamobile 12 ปีที่แล้ว +4

    This was...AMAZING!!!! Thanks for the vid!

  • @gajendraufat4331
    @gajendraufat4331 6 ปีที่แล้ว

    Sir thankyou apke video se bahot help ho jati samjne main ese hi agge hamare liye video banate rhe

  • @MrJazkiller
    @MrJazkiller 13 ปีที่แล้ว

    @DerSchnurrbart yes i agree.

  • @txdude86
    @txdude86 13 ปีที่แล้ว +3

    5 min in xylene is all u need to get rid of the paraffin and just a min each in the other reagents

    • @febriyansyahsaputra
      @febriyansyahsaputra 7 ปีที่แล้ว

      may I have your protocol ? please email me to ryansaputra252@gmail.com

  • @yashusingh5244
    @yashusingh5244 2 ปีที่แล้ว

    Thankyou so much sir

  • @zeinabrahnama5749
    @zeinabrahnama5749 11 หลายเดือนก่อน

    Soo helpful thankss

  • @marialima334
    @marialima334 5 ปีที่แล้ว

    saaai tá tudo errado

  • @maheshpatidar6125
    @maheshpatidar6125 7 ปีที่แล้ว

    Thanku so much

  • @JoyJaps-g9l
    @JoyJaps-g9l ปีที่แล้ว

    Fantastic
    And easier

  • @Azander137
    @Azander137 13 ปีที่แล้ว +1

    Is this how babies are made

    • @madhanprasathr247
      @madhanprasathr247 4 ปีที่แล้ว

      Nope you are wrong

    • @Azander137
      @Azander137 4 ปีที่แล้ว +2

      @@madhanprasathr247 Excellent, thank you for the response. Unfortunately I needed the answer to this nearly a decade ago...

    • @madhanprasathr247
      @madhanprasathr247 4 ปีที่แล้ว

      @@Azander137 sry bruh before a decade i was just a school kiddo 😂

    • @ذيبالشمال-ث9ب
      @ذيبالشمال-ث9ب 3 ปีที่แล้ว

      @@Azander137 n
      this medicine
      how we get sections tissue

  • @TheRealHarsjan
    @TheRealHarsjan 13 ปีที่แล้ว

    @MrJazkiller oo ok thanks very much indeed :-)

  • @ricasaluria8821
    @ricasaluria8821 4 ปีที่แล้ว

    Hello, what are the most widely used fixative in HandE staining method
    A. Formaldehyde
    B.zenker formol
    C. Picric acid
    D. Osmic acid
    Please help me answer

    • @LAHM-TUBE
      @LAHM-TUBE 4 ปีที่แล้ว

      I don't know the most widely but I used Formaldehyde to fixed gills and stomach of fish.

    • @tajmuhammad3715
      @tajmuhammad3715 4 ปีที่แล้ว

      Formaldehyde