Methods of creating sticky ends | Linkers, adaptors and homopolymer tailing | Stick and blunt ends

[@alisharao6902]

Its so much clearer after your lecture sir…i have been seeing many videos regarding to this topic but yours was the best

[@drneerajkumar]

Thanks for your feedback

[@pratikshakesarkar5435]

Wonderful ....crystal clear explanation....
Thank you so much sir 🙂

[@drneerajkumar]

Thanks for the feedback

[@muhammadidrees7823]

For the very first time ever i am leaving my complement.. very clear after watching ur lecture..

[@Funnyvibesanil]

Thank you sir. Now it's very much clearer to me.

[@filmazia4814]

Bhae wah shukariya

[@studyonline-kz1gf]

Linkers is a synthetic short and known double stranded oligonucleotide sequences having blunted ends on both side and also have restrictions sites

[@aryasahay]

Thankyou Sir, this is a very clear explanation.👍🏻

[@johnlopez2028]

Hi! what would be the length of the homopolymer tail that would be produce after treating a DNA fragment with TdT and dNTPs? How do we control the number of nucleotides added/polymerized by TdT?

[@asmitabhattacharya7206]

Sir how ligation is possible between blunt ended dna and sticky ends of adaptors

[@rajninehra485]

Thanku so much sir🙏

[@Pawar-k4e]

Very helpful sir thanks 👍

[@sonishayna249]

Very nice video sir...it helped me alot..can u make a video on plasmid vector marker n all like of (biotechnology 12th in brief)

[@drneerajkumar]

Thanks for your feedback. You can see plasmid vector video in Recombinant DNA Technology playlist. Many important topics of Biotechnology are also there.

[@drneerajkumar]

Plasmid vectors video link
th-cam.com/video/5Z-oyy9JhZw/w-d-xo.html

[@studyonline-kz1gf]

By using linkers we produced sticky ends from blunt end DNA molecule

[@yoonmoon1102]

thankyou very much for this

[@navadeepdorai2918]

Thank you sir.

[@studyonline-kz1gf]

Unpaired nucleotide base

[@hussainshaik6036]

Supper sir thank you very much

[@studyonline-kz1gf]

Adaptors

[@studyonline-kz1gf]

Unpaired nucleotide

[@studyonline-kz1gf]

Terminal deoxynucleotidyl transferase