Protein sequencing sample exercise solved: elucidation of disulfide bonds

#protein_sequencing
#sanger_method
#edman_degradation
Problem-solving in Protein Sequencing
Identify the location of the disulfide bridges, interchain (between 2 Cys existing in 2 different subunits) and intrachain (between 2 Cys existing in the same subunit) , within a polypepeptide.
Disulfide bonds formed between two oxidized cysteines
Cys-SH + HS-Cys give Cys- S--S-Cys
You can find below the links to the protein sequencing steps and to another solved exercise
• protein sequencing steps
• protein sequencing steps

[@anjanamohan5352]

Thank you so much from the bottom of heart ❤️

[@aliciabeatris4402]

Thank you your explanation is very clear and helpful

[@BiochemistryNK]

Glad to know that

[@thesanskaarisoul]

Amazing and crisp

[@mdashadujjamannur2362]

thank you very much ❤

[@parthibanezhumalai2949]

Hi Karaki,
How we have to locating disulphide bonds after trypsin cleavage.

[@BiochemistryNK]

you know that s s bonds exists between 2 Cys, so from the fragments you obtained you deduce between which 2 Cys does the s s bond exist